Molecular evaluation of 1RS-rye translocation distribution in the Iranian dryland wheat cultivars and elite promising lines

Document Type : Research Paper


1 Asistant Professor, Dryland Agricultural Research Institute (DARI), Agricultural Research, Education and Extension Organization (ARREO), Maragheh, Iran

2 Former M. Sc. Student of Agricultural Biotechnology, Islamic Azad University, Maragheh, Iran


The short arm of 1RS rye (Secale cereale L.) chromosome carrying genes involved in resistance to biotic and abiotic stresses provided a valuable source in developing new bread wheat (Triticum aestivium L.) cultivars resilient to stressed environments. The 1BL.1RS and 1AL.1RS translocations have been utilized in wheat breeding programs on a global scale. In this study, three rye specific primers (RyeR3/F3, PAW161 and O-SEC5’-A/O-SEC3’-R) were applied to investigate the distribution of 1RS arms in 22 dryland promising wheat genotypes, 7 dryland commercial wheat cultivars (Azar2, Ohadi, Rasad, Sabalan, Karim, Rijaw and Homa) and three genotypes with international origin (Seri82, 21thFW-236, and 21thFW-244) along with positive and negative controls to verify the results. 1RS arm presence was confirmed in the 86% of Iranian dryland promising lines and wheat cultivars. Using O-SEC5’-A/O-SEC3’-R primer none of the examined Iranian dryland wheat lines and cultivars was found to carry 1AL.1RS translocation. In this study Rye specific markers proved effective in the successful detection of wheat cultivars carrying 1RS arms and application of MAS in identifying wheat genotypes tolerant to environmental stresses. A high frequency of 1BL.1RS translocation was detected in the dryland promising wheat lines as well as in the commercial dryland wheat cultivars. This finding suggested the need for diversifying resistance gene sources by introducing the 1AL.1RS translocation into Iranian wheat germplasm. Genetic diversity revealed here, may be explored for developing new wheat cultivars with enhanced level of adaptation to adverse environmental conditions. 


Main Subjects

  1. Afshari, F. (2006). Protein Marker Assisted Identification of Yr9, Lr26 and Sr31 Genes in a Group of Iranian Wheat Cultivars. Journal of Agricultural Science and Technology, 8, 265-268.
  2. Anugrahwati, D. R., Shepherd, K.W., Verlin, D. C., Zhang, P., Mirzaghaderi, G., Walker, E., Francki, M. G. & Dundas, I. S. (2008). Isolation of wheat-rye 1RS recombinants that break the linkage between the stem rust resistance gene SrR and secalin. Genome, 51, 341-349.
  3. Bagherikia, S., Karimzadeh, G. & Naghavi, M. R. (2014). Distribution of 1AL. 1RS and 1BL. 1RS wheat-rye translocations in Triticum aestivum using specific PCR. Biochemical systematics and ecology, 55, 20-26.
  4. Carver, B. F. & Rayburn, A. L. (1994). Comparison of related wheat stocks possessing 1B or 1RS. 1BL chromosomes: agronomic performance. Crop Science, 34, 1505-1510.
  5. Doyle, J. & Doyle, J.  (1987). a rapid DNA isolation procedure for small quantitates of fresh leaf tissue. Phytochemistry Bull, 19, 11-15.  
  6. Friebe, B., Jiang, J., Raupp, W.J., McIntosh, R. A. & Gill, B. S. (1996). Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica, 91, 59-87.
  7. Gill, B. S. & Kimber, G. (1977). Recognition of translocations and alien chromosome transfers in wheat by the Giemsa C-banding technique. Crop Science, 17, 264-266.
  8. Graybosch, R.A. (2001). Uneasy unions: Quality effects of rye chromatin transfers to wheat. Journal of Cereal Science, 33, 3-16.
  9. Heslop-Harrison, J.S., Leitch, A.R., Schwarzacher, T. & Anamthawat-Jonsson, K. (1990). Detection and characterization of 1B/1R translocations in hexaploid wheat. Heredity, 65, 385-392.
  10. Hoffmann, B. (2008). Alteration of drought tolerance of winter wheat caused by translocation of rye chromosome segment 1RS. Cereal Research Communications, 36, 269-278.
  11. Jiang, J., Friebe, B. & Gill, B. S. (1994). Chromosome painting of Amigo wheat. Theoretical and Applied Genetics, 89, 811-813.
  12. Katto, M. C., Endo, T. R. & Nasuda, S. (2004). A PCR-based marker for targeting small rye segments in wheat background. Genes and genetic systems, 79, 245-250.
  13. Kim, W., Johnson, J. W., Baenziger, P. S., Lukaszewski, A. J. & Gaines, C.S. (2004). Agronomic effect of wheat-rye translocation carrying rye chromatin (1R) from different sources. Crop Science, 44, 1254-8.
  14. Kovalchuk, A., Senam, S., Mauersberger, S. & Barth, G. (2005). Tyl6, a novel Ty3/gypsy-like retrotransposon in the genome of the dimorphic fungus Yarrowia lipolytica. Yeast, 22, 979-991.
  15. Landjeva, S., Korzun, V., Tsanev, V., Vladova, R. & Ganeva, G. (2006). Distribution of the wheat–rye translocation 1RS. 1BL among bread wheat varieties of Bulgaria. Plant breeding, 125, 102-104.
  16. Li, L. J., Wang, X. P., Zhong, L. & Xu, X. L. (2006). Study on homologous chromosomes pairing and translocation induced by 5A/5R × 6A/6R wheat-rye substitution lines. Acta Genetica Sinica, 33, 244-250.
  17. Lookhart, G. L., Graybosch, R., Peterson, J. & Lukaszwski, A. (1991). Identification of wheat lines containing the 1BL/1RS translocation by high-performance liquid chromatography. Cereal chemistry, 68, 312-316.
  18. Lukaszewski, A. J. (1990).  Frequency of 1RS. 1AL and 1RS. 1BL translocations in United States wheats. Crop science, 30, 1151-1153.
  19. Merker, A. (1982). ‘Veery’- a CIMMYT spring wheat with the 1B/1R chromosome translocation. Cereal Research Communication, 10, 105-106.
  20. Miller, T. E., Reader, S. M., Purdie, K. A., Abbo, S., Dunford, R. P. & King, I. P. (1995). Fluorescent in situ hybridization as an aid to introducing alien genetic variation in wheat. Euphytica, 85, 275-279.
  21. Mirzaghaderi, G., Zeinali, G., Rafiepour, M. & Karimzadeh, G. (2011). Wheat-rye translocation in Iranian bread wheat cultivars and their ion distribution in response to salinity stress. Journal of Agricultural Science and Technology, 13, 1163-172.
  22. Moreno-Sevilla, B., Baenziger, P.S., Peterson, C. J., Graybosch, R. A. & McVey, D. V. (1995). The 1BL/1RS translocation: agronomic performance of F3-derived lines from a winter wheat cross. Crop Science, 35, 1051-1055.
  23. Nadella, K. D., Peake, A.S., Bariana, H.S., Cooper, M., Godwin, I.D. & Carroll, B. J. (2002). A rapid PCR protocol for marker assisted detection of heterozygotes in segregating generations involving 1BL/1RS translocation and normal wheat lines. Crop and Pasture Science, 53, 931-938.
  24. Nagy, E. D. & Lelley, T. (2003). Genetic and physical mapping of sequence-specific amplified polymorphic (SSAP) markers on the 1RS chromosome arm of rye in a wheat background. Theoretical and Applied Genetics, 107, 1271-1277.
  25. Porter, D. R., Webster, J. A., Burton, R. L., Puterka, G. J. & Smith, E. L. (1991). New sources of resistance to greenbug in wheat. Crop Science, 31, 1502-1504.
  26. Rabinovich, S. V. (1998). Importance of wheat-rye translocations for breeding modern cultivar of Triticum aestivum L. Euphytica, 100, 323-340.
  27. Rayburn, A. L. & Carver, B.F. (1998). Cytological 1B.1R wheat-rye translocations in winter wheat breading lines, Euphytica, 38, 237-240.
  28. Sebesta, E. E., Wood, E. A., Porter, D. R., Webster, J. A. & Smith, E. L. (1995). Registration of amigo wheat germplasms resistant to greenbug. Crop Science, 35, 293-293.
  29. Shimizu, Y., Nasuda, S. & Endo, T. R. (1997). Detection of the Sec-1 locus of rye by a PCR-based method. Genes and genetic systems, 72, 197-203.
  30. Skerritt, J. H. & Lew, P.Y. (1990).  Homologies between grain storage proteins of different cereal species. 1. Monoclonal antibody reaction with total protein extracts. Journal of Cereal Science, 11, 103-121.
  31. Tabibzadeh, N., Karimzadeh, G. & Naghavi, M. (2013). Distribution of 1AL. 1RS and 1BL. 1RS wheat-rye translocations in Iranian wheat, using PCR based markers and SDS-PAGE. Cereal Research Communications, 41, 458-467.
  32. Trubacheeva, N. V., Rosseeva, L. P., Belan, I.A., Osadchaya, T.S., Kravtsova, L. A., Kolmakov, Y. V. & Pershina, L. A. (2011). Characteristics of common wheat cultivars of West Siberia carrying the wheat-rye 1RS. 1BL translocation. Russian Journal of Genetics, 47, 13-18.
  33. Villareal, R. L., Rajaram, S., Mujeeb‐Kazi, A. & Toro, E. D. (1991). The effect of chromosome 1B/1R translocation on the yield potential Triticum turgidum of certain spring wheats (Triticum aestivum L.). Plant Breeding, 106, 77-81.
  34. Weng, Y., Azhaguvel, P., Devkota, R. N. & Rudd, J. C. (2007). PCR‐based markers for detection of different sources of 1AL. 1RS and 1BL. 1RS wheat–rye translocations in wheat background. Plant Breeding, 126, 482-486.
  35. William, M. D. & Mujeeb-Kazi, A. (1993). Rapid detection of 1B, 1BL/1RS heterozygotes in the development of homozygous 1BL/1RS translocation stocks of (2 n= 4 x= 28). Genome, 36, 1088-1091.
  36. Yediay, F. E., Baloch, F. S., Kilian, B. & Özkan, H. (2010). Testing of rye-specific markers located on 1RS chromosome and distribution of 1AL. RS and 1BL. RS translocations in Turkish wheat (Triticum aestivum L., T. durum Desf.) varieties and landraces. Genetic resources and crop evolution, 57, 119-129.
  37. Zeller, F. J. & Hsam, S. L. K. (1983). Broadening the genetic variability of cultivated wheat by utilizing rye chromatin. In: Sakamoto S (ed), Proc 6th Int Wheat Genet Symp. Maruzen, Kyoto: 161-173.
  38. Zhou, Y., He, Z., Zhang, G., Xia, L., Chen, X., Gao, Y., Jing, Z. & Yu, G. (2004). Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agronomica Sinica, 30, 531-535.
  39. Zuniga, J., Sotom B. & Camposm H. (2008). Using a gel‐free PCR‐ELISA for the molecular identification of wheat genotypes carrying wheat–rye translocations. Plant breeding, 127, 15-19.
Volume 48, Special Issue
October 2017
Pages 119-128
  • Receive Date: 27 September 2016
  • Revise Date: 19 August 2017
  • Accept Date: 28 August 2017
  • Publish Date: 23 September 2017