Study of invertases activity and soluble sugar concentration at developmental stages of some broomrape species on tomato

Document Type : Research Paper

Authors

1 Faculty of Agricultural Sciences and Engineering-College of Agriculture and Natural resources-University of Tehran

2 University of Tehran

3 ’Faculty of Agricultural Sciences and Engineering-Faculty members of college of agriculture and natural resources-University of Tehran

Abstract

Broomrape is an obligate parasite in many economically important crops such as tomato. Success in controlling broomrape is rare hence an understanding of sucrose utilization mechanisms as a critical process for parasite provides developing specific control strategies. This parasite acts as a competitive sink for sucrose, utilizes it in the tuber and converts it to glucose and fructose. Invertases are the major enzymes in sucrose degradation and osmotic adjustment in the parasite. In this paper, the activity of two invertases (PrSAI and PrCWI) involved in different developmental stages of some broomrape species (Phelipanche aegyptiaca, P. ramosa and P. nana) on tomato was studied. Results showed that activity of both mentioned enzymes were detected in all of the developmental stages in the broomrape species; although the PrSAI activity was always higher than the PrCWI activity. The activities of PrSAI and PrCWI was increased and decreased, respectively, as progressing of the developmental stages of broomrape. Enzymes activity and protein and sugar amounts in Egyptian broomrape were high at the early stages and in branched broomrape in the late stages, the first case accorded to the earlier emergence of broomrape on the host and the later one accorded to more shoots and higher biomass in broomrape. In total, results suggested that PrSAI1 and, possibly, PrCWI are suitable targets for gene silencing strategies.

Keywords

Main Subjects

References

  1. Abbes, Z., Kharrat, M., Delavault, P., Chaibi, W. & Simier, P. (2009). Nitrogen and carbon relationships between the parasitic weed Orobanche foetida and susceptible and tolerant faba bean lines. Plant Physiology and Biochemistry, 47, 153-159.
  2. Aber, M., Fer, A. & Salle, G. (1983).Transfer of organic substances from the host plant Vicia faba to the parasite Orobanche crenata Forsk. Z. Zeitschrift fur P flanzenphysiologie, 112, 297–308.
  3. Aly, R.(2007). Conventional and biotechnological approaches for control of parasitic weeds. In Vitro Cellular & Developmental Biology - Plant, 43, 304–317.
  4. Aly, R., Cholakh, H., Joel, D. M., Leibman, D., Steinitz, B., Zelcer, A., Naglis, A., Yarden, O. & Gal-On, A. (2009). Gene silencing of mannose 6-phosphate reductase in the parasitic weed Orobanche aegyptiaca through the production of homologous dsRNA sequences in the host plant. Plant Biotechnology Journal, 7, 487-498.
  5. Baghestani, M. A., Behrouzi, D., Rahimian Mashhadi, H. & Mohammad Alizadeh, H. (2009). Assessment of biological and phenological aspects of broomrape (Orobanche nana) in parasitism with Almond (Prunus dulcis). Journal of Crops Improvement, 11, 19-27 (In Farsi).
  6. Bradford, M. (1976). A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein dye binding. Analytical Biochemistry 72, 248–254.
  7. Crescenzi, A., Fanigliulo, A., Fontana, A. & Fascetti, S. (2015). First Report of Orobanche nana on Celery in Italy. (American Phytopathological Society 2015:99). Disease Notes. 1188.
  8. Delavault, P., Simier, P., Thoiron, S., Veronesi, C., Fer, A. & Thalouarn, P. (2002). Isolation of mannose 6-phosphate reductase cDNA, changes in enzyme activity and mannitol content in broomrape (Orobanche ramosa) parasitic on tomato roots. Physiologia Plantarum, 115, 48–55.
  9. Draie, R., Peron T., Pouvreau, J., Veronesi, C., Jegou, S., Delavault, P., Thoiron, S. & Simier, P. (2011). Invertases involved in the development of the parasitic plant Phelipanche ramosa: Characterization of the dominant soluble acid isoform, PrSAI1. Molecular Plant Pathology, 12, 638-652.

10. El-Hamouch, Y., Benharrat, H. & Thalouran, P. (2006). Effect of root exudates from different tomato genotypes on broomrape (O. aegyptiaca) seed germination and tubercule development. Crop Protection, 25, 501-507.

11. Fer, A., Russo, N., Simier, P., Arnaud, M. C., Thalouarn, P. (1993). Physiological changes in a root hemiparasitic angiosperm, Thesium humile (Santalaceae), before and after attachment to the host plant (Triticum vulgare). Journal of Plant Physiology, 20,15-24.

12. Goldwasser, Y. & Kleifeld, Y. (2004). Recent approaches to Orobanche management: A review. In: Inderjit (Ed), Weed Biology and Management. (pp. 439–566.) Kluwer Academic Publishers.

13. Hacham, Y., Hershenhorn, J., Dor, E. & Amir, R. (2016). Primary metabolic profiling of Egyptian broomrape (Phelipanche aegyptiaca) compared to its host tomato roots. Journal of Plant Physiology, 205, 11–19.

14. Harloff, H. J. & Wegmann, K. (1987). Mannitol pathway in Orobanche. In: Weber HC, Forstreuter W., eds. Parasitic Flowering Plants, Proceedings of the 4th ISPFP, pp. 295-309.

15. Hershenhorg, J., Eizenberg, H., Dor, E., Kapulnik, Y. & Goldwasser, Y. (2009). Phelipanche aegyptiaca management in tomato. Weed Research, 49, 34-37.

16. Kokina S.I. (1946). Izvest. Turkm. Fil. Akad. Nauk SSSR, 62 (314). Abstract: Information Section, Weed Research Organization, A.R.C.

17. Nie, P., Wang, X., Hu, L., Zhang, H., Zhang, J., Zhang, Z. & Zhang L. (2010) .The Predominance of the apoplasmic phloem-unloading pathway is interrupted by a symplasmic pathway during chinese  jujube fruit development. Plant Cell Physiology, 51, 1007–1018.

18. Noiraud, N., Maurousset, L. & Lemoine, R. M. (2001). Transport of polyols in higher plants. Plant Physiology and Biochemistry, 39, 717–728.

19. Pageau, K., Simier, P., Naulet, N., Robins, R. & Fer, A. (1998). Carbon dependency of the hemiparasite Striga hermonthica on Sorghum bicolor determined by carbon isotopic and gas exchange analyses. Australian Journal of Plant Physiology, 25, 695-700.

20. Pan, Q., Zou, K., Peng, C., Wang, X., & Zhang, D. (2005). Purification, biochemical and immunological characterization of  acid invertases from apple fruit. Journal of Integrative Plant Biology, 47, 50-59.

21. Parker, C. &Riches, C. R. (1993). Parasitic Weeds of the World. Biology and Control. Wallingford, UK: CAB International. 332 pp.

22. Simier, P., Renaudin, S. & Fer, A. (1994). Characteristics of the mannitol pathway in a root hemiparasitic species, Thesium humile Vahl. (Santalaceae). Journal of Plant Physiology, 143, 33–38.

23. Singh, M., Singh, D. V., Misra, P. C., Tewari, K. K. & Krishnan, P. S. (1968). Biochemical Aspects of Parasitism by the Angiosperm Parasites: Starch Accumulation. Physiologia Plantarum, 21: 525–538.

24. Wakabayashi, T., Joseph, B., Yasumoto, S., Akashi, T., Aoki, T., Harada, K., Muranaka, S., Bamba, T., Fukusaki, E., Takeuchi, Y., Yoneyama, K., Muranaka, T., Sugimoto, Y. & Okazawa, A. (2015). Planteose as a storage carbohydrate required for early stage of germination of Orobanche minor and its metabolism as a possible target for selective control. Journal of Experimental Botany, 66, 3085–3097.

25. Westwood, J. H., de-Pamphilis, C. W., Das, M., Fernandez-Aparacio, M., Honaas, L. A., Timko, M. P., Wafula, E. K., Wickett, N. J. & Yoder, J. I. (2012). The parasitic plant genome project: New tools for understanding the biology of Orobanche and Striga. Weed Science, 60, 295-306.

26. Whitney, P. J. (1972). The carbohydrate and water balance of beans (Vicia faba) attacked by broomrape (Orobanche crenata). Annals of Applied Biology, 70, 59-66.

27. Zhang, X., Wang, X., Wang, X., Xia, G., Pan, Q., Fan, R., Wu, F., Yu, X. & Zhang, D. (2006). A shift of phloem unloading from symplasmic to apoplasmic pathway is involved in developmental onset of ripening in grape berry. Plant Physiology, 142(6), 220–232.

Iranian Journal of Field Crop Science
Volume 49, Issue 2
August 2018
Pages 71-79

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History

  • Receive Date: 06 June 2017
  • Revise Date: 03 July 2017
  • Accept Date: 10 July 2017
  • Publish Date: 23 July 2018

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