A promising application of drought stress for increasing product quality of Iranian endemic Satureja sahendica Bornm medicinal plant

Document Type : Research Paper


1 1- ّFormer PhD student, Department of Plant Breeding, Faculty of Agriculture, Tarbiat Modares University, Tehran P. O Box 14115-336, Iran 2- PhD researcher of Research Institute of Forests and Rangelands of Iran, Tehran, Iran.

2 Associate Professor/Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University (TMU)

3 Prof. Research Institute of Forests and Rangelands of Iran, Tehran, Iran.

4 Assoc. Prof., Department of Agricultural Engineering, Medicinal Plants and Drug Research Institute, Shahid Beheshti, University, G.C. Evin, Tehran, Iran.


Sahendian savory (Satureja sahendica Bornm.) is an Iranian endemic species from Lamiaceae family. This plant has been used in the food industry, cosmetics and medical preparations. In the current study, drought stress was induced at flowering stage based on a completely randomized design (CRD) with three replications in green house. Treatments were considered as five sampling times (control, 3, 6, 9 and 12 days) with three interval days that imposed after stopping irrigation. Soil volumetric moisture, and several physiological traits were measured, including leaf water potential, relative water content, pigments, soluble sugars, and proline. Metabolite profiling revealed that metabolites, such as rosmarinic acid, caffeic acid, ursolic acid, carnosic acid, soluble sugars and proline affected by drought stress and significantly increased by drought stress. The oil yield and thymol as the most valuable compound in the oil of Sahandian savory, was significantly increased, although, the quantitative content of some compounds in oil such as Carvacrole, γ-Terpinene and p- Cymene were decreased in response to drought stress. It can be concluded that in addition to osmoprotectant accumulation, savory plant improved its drought tolerance by changing in its secondary metabolites’ components in essential oil and in extract. In conclusion, the combination of metabolite profiling and physiological parameters contributed to a greater understanding of the mechanisms of savory plant’s response at metabolomics level.


Main Subjects

  1. Abbaszadeh, B., Sharifi Ashourabadi, E., Lebaschi M.H., Naderi Haji Bagherkandi, M. & Moghadami, F. (2008).The effect of drought stress on proline contents, soluble sugars, chlorophyll and relative water contents of balm (Melissa officinalis L.). Iranian Journal of Medicinal and Aromatic Plants, 38(4), 504-513.
  2. Adams, R.P. (2007). Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th edn. Allured Publishing Corporation, Carol Stream, IL, USA.
  3. Akbarinia, A., Sefikon, F. & Razaz Hashemi, S.R. (2009). Essential oil components of cultivated and wild accessions of Satureja sahendica Bornm. Iranian Journal of Medicinal and Aromatic Plants, 25(3), 376-385.
  4. Baskan, S., Oztekin, N. & Erim, F. (2007). Determination of carnosic acid and rosmarinic acid in sage by capillary electrophoresis. Food Chemistry, 101, 1748-1752.
  5. Bates, I.S., Waldern, R.P. & Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205-207.
  6. Bartels, D. & Sunkar, R. (2005). Drought and salt tolerance in plants. Critical Reviews in Plant Sciences, 24, 23-58.
  7. Boyer, J.S. 1968. Measurement of the water status of plants. Annual Review of Plant Physiology, 9, 351-363.
  8. Brosché, M., Vinocur, B., Alatalo, E.R., Lamminmäki, A., Teichmann, T., Ottow, E. & Kangasjärvi, J. (2005). Gene expression and metabolite profiling of Populus euphratica growing in the Negev desert. Genome Biology, 6(12), R101.
  9. Chaves, M.M. (1991). Effects of water deficits on carbon assimilation. Journal of Experimental Botany, 42, 1-6.

10. Dashti, M ., Kafi, M ., Tavakoli, H . & Mirza, M . (2014). Effect of Water Deficit on Water Relations, Photosynthesis and Osmolytes Accumulation of Salvia leriifolia Benth. Iranian Journal Of Field Crops Research, 12, 813-821.

11. Dawalibi, V., Monteverdi, M., Moscatello, S., Battistelli, A. & Valentini, R. (2015). Effect of salt and drought on growth, physiological and biochemical responses of two Tamarix species. iForest - Biogeosciences and Forestry, e1–e8.

12. De Abreu, I.N. & Mazzafera, P. (2005). Effect of water and temperature stress on the content of active constituents of Hypericum brasiliense Choisy. Plant Physiology and Biochemistry, 43, 241-248.

13. Foito, A. (2010). A Metabolomics-Based Approach to Study Abiotic Stress in Lolium perenne. Ph.D. Thesis, University of Dundee, Dundee, Scotland, UK.

14. Grace, S.C. & Logan, B.A. (2000). Energy dissipation and radical scavenging by the plant phenylpropanoid pathway. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 355, 1499-1510.

15. Gratao, P.L., Polle, A., Lea, P.J. & Azevedo, R.A. (2005). Making the life of heavy metal-stressed plants a little easier. Functional Plant Biology, 32, 481-494.

16. Hadian, J., Ebrahimi, S.N. & Salehi, P. (2010). Variability of morphological and phytochemical characteristics among Satureja hortensis L. accessions of Iran. Industrial Crops and Products, 32(1), 62-69.

17. Hendry, G.A.F. & Wallace, R.K. (1993). The origin, distribution and evolutionary significance of fructans. In: Suzuki M. & Chatterton, J.N. (eds.), Science and Technology of Fructans. CRC Press, Boca Raton, USA, pp. 119-139.

18. Ho, S., Chao, Y., Tong, W. & Yu, S. (2001). Sugar coordinately and differentially regulates growth and stress-related gene expression via a complex signal transduction network and multiple control mechanisms. Plant Physiology, 46, 281-285.

19. Irigoyen, J.J., Eineric, D.W. & Sanchez-Diaz, M. (1992). Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84 (1), 58-60.

20. Jamzad, Z. (2009). Thymus and Satureja species of Iran, Research Institute of Forests and Rangelands Publication, Tehran, Iran, 76 p.

21. Jason, A. (1978). Chlorophyll and Cartenoid: Handbook of Physiological Method. Cambridge University Press, Cambridge, UK, pp. 59-65.

22. Krasensky, J. & Jonak, C. (2012). Drought, salt, and temperature stress-induced metabolic rearrangements and regulatory networks. Journal of Experimental Botany, 63: 1593-1608.

23. Liang, Z., Jiang, Z., Fong, D.W. & Zhao, Z. (2009). Determination of oleanolic acid and ursolic acid in Oldenlandia diffusa and its substitute using high performance liquid chromatography. Journal of Food and Drug Analysis, 17(2), 69-77.

24. Lutts, S.J., Kint, M. & Bouharmount, J. (1996). Effect of various salts and mannitolon ion and proline accumulation in relation to osmotic adjustment in rice (Oryza sativa) callus cultures. Journal of Plant Physiology, 149, 186-195.

25. Magel, E., Mayrhofer, S., Müller, A., Zimmer, I., Hampp, R. & Schnitzler, J.P. (2006). Photosynthesis and substrate supply for isoprene biosynthesis in poplar leaves. Atmospheric Environment, 40, 138-151.

26. Manukyan, A. (2011). Effect of growing factors on productivity and quality of lemon catmint, lemon balm and sage under soilless greenhouse production: I. Drought stress. Medicinal and Aromatic Plant Science and Biotechnology, 5, 119-125.

27. Michel, B.E. (1972). Solute potentials of sucrose solutions. Plant Physiology, 50, 196-198.

28. Moradi, P. (2014). Use of metabolomics to study water deficit stress on the medicinal plant thyme. Ph.D. Thesis, University of Birmingham, Birmingham, UK.

29. Nowak, M., Manderscheid, R., Weigel, H. J., Kleinwachter, M. & Selmar, D. (2010). Drought stress increases the accumulation of monoterpenes in sage (Salvia officinalis), an effect that is compensated by elevated carbon dioxide concentration. Journal of Applied Botany and Food Quality, 83, 133-136.

30. Radwan, A. (2014). The impact of drought stress on monoterpene biosynthesis in sage (Salvia officinalis): Dehydrins and monoterpene synthases as molecular. Thesis, Technische Universität Carolo-Wilhelmina Zu Braunschweig. Braunschweig, Germany.

31. Reich, E. & Schibli, A. (2006). High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants. Thieme Medical Pub, New York, USA, 197 p.

32. Rezaei Chiyaneh, E ., Zehtab Salmasi, S ., Ghassemi Golezani, K . & Delazar, A . (2012). Physiological responses of fennel (Foeniculum vulgare L.) to water limitation. Agroecology, 4, 347-355.

33. Rezaei, H., Ghorbanli, M., Peivandi, M. & Pazoki, A. (2013). Effect of drought interactions with ascorbate on some biochemical parameters and antioxidant enzymes activities in Dracocephalum moldavica L. Middle-East Journal of Scientific Research, 13(4), 522-531.

34. Sarajuoghi, M., Abbaszadeh, B. & Ardakani, M.R. (2014). Investigation morphological and physiological response of Thymus vulgaris L. to drought stress, Journal of Biodiversity and Environmental Sciences, 5(2), 486-492.

35. Shalata, A., Mittova, V., Volokita, M., Guy, M. & Tal, M. (2001). Response of the cultivated tomato and its wild salt tolerant relative Lycopersicon pennellii to salt dependent oxidative stress: the root antioxidative system. Physiologia Plantarum, 112, 487-494.

36. Shulaev, V. (2006). Metabolomics technology and bioinformatics. Brief Bioinform, 7, 128-139.

37. Taji, T., Ohsumi, C., Iuchi, S., Seki, M., Kasuga, M., Kobayashi, M., Yamaguchi-Shinozaki, K. & Shinozaki, K. (2002). Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana. Plant Journal, 29, 417-426.

38. Weckwerth, W. (2007). Metabolomics, methods and protocols. Humana Press, New Jersey, USA.

39. Weckwerth, W. & Kahl, G. (2013). The Handbook of Plant Metabolomics. 1st edn. Oxford: Wiley-Blackwell, UK.

40. Wilhelm, C. & Selmar, D. (2011). Energy dissipation is an essential mechanism to sustain the viability of plants: The physiological limits of improved photosynthesis. Journal of Plant Physiology, 168, 79-87.

41. Wink, M. (2010). Introduction: biochemistry, physiology and ecological functions of secondary metabolites. In: Wink, M. (Ed.), Biochemistry of Plant Secondary Metabolism. pp. 1-19. Wiley Blackwell, Oxford, UK.

42. Yousefzadi, M., Riahi-Madvar, A., Hadian, J., Rezaee, F., & Rafiee, R. (2012). In vitro cytotoxic and antimicrobial activity of essential oil from Satureja sahendica. Toxicological and Environmental Chemistry, 94, 1735-1745.



Volume 49, Issue 1
June 2018
Pages 167-177
  • Receive Date: 01 March 2017
  • Revise Date: 25 May 2017
  • Accept Date: 13 September 2017
  • Publish Date: 22 May 2018