Evaluation of freezing tolerance in faba bean (Vicia faba L.) using electrolytes leakage index

Document Type : Research Paper

Authors

1 Faculty of Agriculture, Ferdowsi University of Mashhad

2 Department of Agronomy and Plant Breeding, Faculty of Agriculture and Research Center for Plant Sciences, Ferdowsi University of Mashhad

Abstract

Autumn planting leads to improve the yield of cool season crops, however there is little information about the cold tolerance in national faba bean landraces for autumn planting. For this purpose a study was conducted as factorial, based on completely randomized design with four replications under controlled conditions, and two faba bean landraces (Boroujerd and Neyshabour) exposed to freezing temperatures (0, -4, -8, -12, -16, -20, -24°C). Results showed that decreasing temperature leads to the increasing EL% in all organs, however decreasing temperature hadn't significant effect on EL% up to -12°C in leaf and meristem and EL% increased with reducing the temperature to reached to the maximum in -20°C, While in stem EL% increasing was started from -8°C and reached to the maximum in -16°C. In both of Boroujerd and Neyshabour landraces, stem had the highest LT50el (-12.9 and -12.1, respectively), while it was -16.7 and -15.6°C in leaf and -16.0 and -16.8°C in meristem of two mention landraces, respectively. Although there was negative and significant correlation between the EL% of faba bean organs with SU%, but leaf and stem in Neyshabour landrace in similar LT50su with boroujerd landrace (-13.6°C) had more EL%, while it was further in the meristem of Boroujerd landrace. Two faba bean landraces showed ability to tolerate freezing temperatures up to -12°C in controlled conditions.

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Main Subjects


  1. Ali, M. B., Welna, G., Sallam, A., Martsch, R., Balko, CH., Gebser, B., Sass, O. & Link, W. (2016). Association analyses to genetically improve drought and freezing tolerance of faba bean (Vicia faba L.). Crop Science, 56, 1036-1048.
  2. Anderson, J. A., Michael, P. & Taliaferro, C. M. (1988). Cold hardiness of midiron and tifgreen. Horticultural Science, 23, 748-750.
  3. Arbaoui, M., Balko, C. & Link, W. (2008). Study of faba bean (Vicia faba L.) winter-hardiness and development of screening methods. Field Crops Research, 106, 60-67.
  4. Armoniene, R., Liatukas, Z. & Brazauskas, G. (2013). Evaluation of freezing tolerance of winter wheat (Triticum aestivum L.) under controlled conditions and in the field. Zemdirbyste-Agriculture, 100, 417-424.
  5. Asadi-Sanam, S., Zavareh, M., Pirdashti, H., Sefidkon, F. & Nematzadeh, GH.A. (2015). The physiological and biochemical responses of purple coneflower (Echinacea purpurea (L) Moench) to low temperature stress. Journal of Plant Process and Function, 4, 11-28. (in Farsi)
  6. Calzadilla, P. I., Maiale, S. J., Ruiz, O. A. & Escaray, F. J. (2016). Transcriptome response mediated by cold stress in Lotus japonicus. Frontiers in Plant Science, 7, 1-16.
  7. Cardona, C. A., Duncan, R. R. & Lindstrom, O. (1997). Low temperature tolerance assessment in paspalum. Crop Science, 37, 1283-1291.
  8. Eugenia, M., Nunes, S. & Ray Smith, G. 2003. Electrolyte leakage assay capable of quantifying freezing resistance in rose clover. Crop Science, 43, 1349-1357.
  9. Fan, F., Zhang, F., Song, Y, Sun, J., Bao, X., Guo, T. & Li, L. (2006). Nitrogen fixation of faba bean (Vicia faba L.) interacting with a non-legume in two contrasting intercropping systems. Plant and Soil, 283, 275–286.

10. FAO. (2005). Frost protection: fundamentals, practice, and economics. www.FAO.com.

11. FAO. (2016). Nutritional Benefits of Pulses. www.FAO.com.

12. Hajmohammadnia- Ghalibaf, K., Nezami, A. & Kamandi, A.(2010). Study the possibility of using the electrolyte leakage index for evaluation of cold tolerance in sugar beet (Beta vulgaris L.) cultivars. Iranian Journal of Field Crops Research, 8, 465-472. (in Farsi)

13. Hamzei, J. & Ghamari-rahim, N. (2016). Evaluation of economic Faba bean (Vicia faba L.) intercropping with maize (Zea mays L.) based on relative total value and reduce weeds growth. Journal of Crop Production and Processing, 6, 97-108. (in Farsi)

14. Kafi, M., Kamkar, B. & Mahdavi-Damghani., A. M. (2003). Crop responses to environment. Ferdowsi University Press. 297p.

15. Kim, T. E., Kim, S. K., Han, T. J., Lee, J. S. & Chang, S. C. (2002). ABA and polyamines act independently in primary leaves of cold‐stressed tomato (Lycopersicon esculentum). Physiologia Plantarum, 115, 370-376.

16. Kratsch, H. A. & Wise, R. R. (2000). The ultrastructure of chilling stress. Plant, Cell and Environment, 23, 337-350.

17. Lindow, S. E., Arny, D. C. & Upper, C. D. (1982). Bacterial ice nucleation: a factor in frost injury to plants. PlantPhysiology, 70, 1084-1089.

18. Link, W., Balko, C. & Stoddard, F. L. (2010). Winter hardiness in faba bean: Physiology and breeding. Field Crops Research, 115, 287-296.

19. Longo, V., Valizadeh-Kamran, R., Michaletti, A., Toorchi, M., Zolla, L. & Rinalducci, S. (2017). Proteomic and physiological response of spring barley leaves to cold stress. International Journal of Plant Biology and Research, 5, 1061.

20. Majnonhosseini, N. (2008). Grain legume production. Tehran Jahad Daneshgahi Publications. 294p.

21. McDermott, J. & Wyatt, A. J. (2017). The role of pulses in sustainable and healthy food systems. Annals of the New York Academy of Sciences, 1392, 30-42.

22. Mousavi, M. J., Nezami, S., Izadi Darbandi, E., Nezami, A., Yousefsani, M. & Keykha Akhar, F. (2011). Evaluation of freezing tolerance of english daisy (Bellis perennis) under controlled conditions. Journal of Water and Soil, 25, 380-388. (in Farsi)

23. Nezami, A., Azizi, G., Siahmarghooee, A. &  Mohamadabadi, A. A. (2010). Effects of freezing stress on electrolytes leakage of fennel (Foeniculum vulgare). Iranian Journal of Field Crops Research, 8, 587-593. (in Farsi)

24. Nezami, A., Rezaei, J. & Alizadeh, B. (2010). Evaluation of cold stress tolerance in several species of grasses by electrolytes leakage test. Journal of Water and Soil, 24, 1019-1026. (in Farsi)

25. Nezami, A., Rezvan-Bidokhti, SH. & Sanjani, S. (2016). The response of saffron (Crocus sativus L.) to freezing stress in controlled conditions. Journal of Environmental Stresses in Crop Sciences, 9, 75-86. (in Farsi)

26. Parsa, M. & Bagheri, A. R. (2008). Pulses. Mashhad Jahad Daneshgahi Publications. 522p.

27. Pietsch, G. M., Anderson, N. O. & Li, P. H. (2009). Cold tolerance and short day acclimation in perennial Gaura coccinea and G. drummondii. Scientica Horticulture, 120, 418-425.

28. Rife, C.L. & Zeinali, H. (2003). Cold tolerance in oilseed rape over varying acclimation durations. Crop Science, 43, 96-100.

29. Rezvan-bidokhti, Sh., Nezami, A., kafi, M. & khazaei, H.R. (2011). The effect of freezing stress on electrolytes leakage in industrial and Medicinal plant shallot (Alliumm altissimum Regel.) under controlled conditions. Journal of Agroecology, 3, 371-382. (in Farsi)

30. Sanghera, G. S., Wani, S. H., Hussain, W. & Singh, N. B. (2011). Engineering cold stress tolerance in crop plants. Current Genomics, 12, 30-43.

31. Thalhammer, A., Hincha, D.K. & Zuther, E.( 2014). Measuring freezing tolerance: electrolyte leakage and chlorophyll fluorescence assays. Plant Cold Acclimation: Methods and Protocols, Methods in Molecular Biology, 1166, 15-24.

32. Uemura, M., Tominaga, Y., Nakagawara, CH., Shigematsu, S., Minami, A. & Kawamura, Y. (2006). Responses of the plasma membrane to low temperatures. Physiologia Plantarum, 126, 81-89.

33. Venaei, S., Siosemarde, A. & Heydari, Gh. R. (2011). The effects of cold stress in germination stage and seedling on the activity antioxidant enzymes and some Physiological traits in pea (Cicer arietinum). Iranian Journal of Field Crops Research, 9, 514-524. (in Farsi)

34. Xuan, J., Liu, J., Gao, H., Hu, H. & Cheng, X. (2009). Evaluation of low-temperature tolerance of Zoysia grass. Tropical Grasslands, 43, 118-124.

35. Yadav, S.K. (2010). Cold stress tolerance mechanisms in plants. A review. Agronomy Sustainable Development, 30, 515-527.

36. Zhang, X., Ervin, E. H. & Labranche, A. J. (2006). Metabolic defense responses of seeded bermudagrass during acclimation to freezing stress. Crop Science, 46, 2598-2605.

37. Zhang, X., Teixeira da Silva, J. A., Niu, M., Li, M., He, Ch., Zhao, J., Zeng, S., Duan, J. & Ma, G. (2017). Physiological and transcriptomic analyses reveal a response mechanism to cold stress in Santalum album L. leaves. Scientific Reports, 7, 42165.