Study of the effects of drought stress on seed germination factors and activity of antioxidant enzymes in Bean genotypes (Phaseolus vulgaris L.)

Document Type : Research Paper


1 Postgraduate / University of Tehran

2 Professor of University of Tehran

3 Associate Professor ? University of Tehran


Drought stress is one of the major causes of reducing crop improvement. To evaluate the effects of drought tolerance on seed characteristics the tolerant and sensitive seed genotypes of common bean were tested in petri dishes containing polyethylene glycol 6000 with concentration equal to 0, -4, -8 and -12 bar, according to CRD design with three replications in factorial arrangement. 9 day later, radice and caulice lengths were scored. The results showed that drought stress decreased rate and percentage of germination, radice and caulice lengths, fresh and dry weight of radices, and fresh and dry weight of caulice. To study biochemical processes in germination stages, genotypes 18, 25, and 29 and commercial variety of Yas were screened for polyethylene glycol 6000 with concentration equal to 0, -4, -8 and -12 bar. By evaluating of the studied traits and indices, with considering physiological and biochemical characteristics, it could be conclude that genotype 1189 was drought tolerant one in seedling stage in comparison to the rest of genotypes and Yas as a check variety.


Main Subjects

  1. Abedi, T. & Pakniat, H. (2010). Antioxidant Enzyme Changes in Response to Drought Stress in Ten Cultivars of Oilseed Rape (Brassica napus L.). Czech Journal of Genetics of Plant Breed, 46(1), 27-34.
  2. Asada, K. (1992). Ascorbate peroxidase-a hydrogen peroxide-scavenging enzyme in plants. Jornal  of Physiologia Plantarum, 85(2), 235-241
  3. Asgharipour, M. R. (2011). Effect of salinity on germination and seedling growth of   lentils. Australian Journal of Basic and Applied Sciences, 5(11), 2002-2004.
  4. Ashraf, M. A. (1988). Salt tolerance of mungbean at two growth stage. Jornal of Plant and Soil, 110, 63-67.
  5. Baalbaki, R. Z., Bleik, M. M. & Talhouk, S. N.  (1999). Germination and seedling development of drought tolerant and susceptible wheat under moisture stress.Seed Science and Technology, 27, 291-302.
  6. Cellier, F., Conéjéro, G., Breitler, J. C. & Casse, F. (1998). Molecular and Physiological Responses to Water Deficit in Drought-Tolerant and Drought-Sensitive Lines of Sunflower: Accumulation of Dehydrin Transcripts Correlates with Tolerance. Jornal of Plant Physiology, 116(1), 319-328.
  7. Chance, B. & Maehly A. C. (1955). Assay of catalases and peroxidases. Methods in Enzymology, Academic Press. Volume 2, 764-775.
  8. Cokjizgin, A. (2012). Salinity Stress in Common Bean (Phaseolus vulgaris L.) Seed Germination. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 40(1), 182-177.
  9. FAO (2010). World Food and Agriculture. FAO STATISTICAL YEARBOOK
  10. Giannopolitis, C. N. & Ries, S. K. (1977). Superoxide dismutases: I. Occurrence in higher plants. Journal of Plant Physiology, 59(2), 309-314.
  11. Golparvar, A., Ghannadha, M. R. & Zali, A. A. (2003). Determine of the best selection traits for yield improvement of bread wheat under drought stress. Seed and Plant Improvement Journal, 18, 144-155.
  12. Ghorbani Ghozhdi, H. (2005). Grapes. In: H. Ghorbani Ghozhdi & A. Ladanmoghadam, Intraduction to oxidative stresses and palnt strians. (in Farsi)
  13. Jajarmi, V. (2012). Effect of drought stress on germination indices in seven wheat cultivars (T. aestivum L.). Journal of Agriculture and Plant Breeding, 8(2), 183-192. (in Farsi)
  14. Jolaei, M. (2005). The effect of mild water stress on some physiological characteristics and the level of enzyme activity of anti-oxidants of different cultivars of wheat. M.Sc. thesis, College of Ag riculture, University of Tehran, Karaj, Iran. (in Farsi)
  15. Kafi, M. & Masoumi, A. (2005). The physiological effects of water stress by PEG6000 on germination of lentil (Lens culinarisL.) genotypes. Iranian Journal of Field Crops Research. (in Farsi)
  16. Kafi, M., Bagheri, A., Nabati, J., Zare Mehrjerdi, M. & Masoumi, A. (2010). Study of salinity stress effect on some physiological variables of 11 chickpea (Cicer arietinum L.) genotypes in hydroponic environments. Journal of Greenhouse Culture Science and Technology, 4, 55-69. (in Farsi)
  17. Maguire, J. D. (1962). Speed of germination. Aid in selection and evaluation of seedling emergence and vigor. Jornal of Crop Science, 2, 176-177.
  18. Mayer, A. M. (1989). The germination of seeds. Pergamon Press, New York, 270 p.
  19. Miklas, P. N., Kelly, J. D., Beebe, S. E. & Blair, M. W. (2006). Common bean breeding for resistance against biotic and abiotic stresses: From classical to MAS breeding. Euphytica, 147(1), 105-131.
  20. Meloni, D. A., Oliva, M. A., Martinez, C. A. & Cambraia, J.  (2003). Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Journal of Plant Physiology, 15(2), 12-21.
  21. Morales, R., C., Duran-Aniotz, J., Castilla, L., Estrada, D. & Soto, C. (2012). De novo induction of amyloid-beta depositionin vivo.Molecular Psychiatry, 17(12), 1347-1353.
  22. Mostafavi, K. (2011). An Evaluation of Safflower Genotypes (Carthamus tinctorius L.), Seed Germination and Seedling Characters in salt Stress conditions. African Journal of Agricultural Resarch, 7, 1667-1672.
  23. Michel, B. E. (1983). Evaluation of the water potentials of solutions of polyethylene glycol 8000 in both the absence and presence of other solutes. Plant Physiology, 72, 66-70.
  24. Oskoui, B. (2010).  Effect of drought stress on some cultivars of wheat cultivars (T. aestivum L.)  in growth stage. In: Proceedings of11th Ntional Iranian Crop Science Congress. SHahid Beheshti University. (in Farsi)
  25. Paulsen, M. R. (1978). Fracture resistance of soybeans to compressive loading. Transactions of ASAE, 1210-1216.
  26. Pessaraki, M. (1996). Plant and crop stress. Handbook. New York.
  27. Romesburg, H. C. (1990).  Cluster Analaysis. For Research. Krieger Pub., Malabar, Florida, 85, 246-253.
  28. Shekari, F., Rahimzade Khoii, F., Valizade, M., Alyari, H. & SHakiba, R. (1997). Effect of salinity stress on germination in oilseed rape (Brassica napus L.). In: Proceedings of5th National Iranian Crop Science Congress. Karaj. 45. (in Farsi)
  29. Saidi, M. (2007). Effect of osmotic stress on germination indices in wheat different genotype (T. aestivum L.).  Jornal of Science and Technology of Agriculture and Natural Resource. (in Farsi)
  30. Salehifar, M. (2010). Compare the effect of drought stress on germination and seedling growth of eight Genetics beans (Phaseolus vulgaris L.).In: Proceedings ofthe 11th Ntional Iranian Crop Science Congress. SHahid Beheshti University. (in Farsi)
  31. Sarmadniya, A. (1987). Study of Drought resistance of the masses of wheat (T. aestivum L.) on germination stage. In: Proceedings of1st International Congress on Research dry land issues in Iran, Mashhad Ferdowsi University. (in Farsi)
  32. Sepanlo, M. G. H. & Siyadat, H. (1998). Effects of water stress on germination characteristics of wheat (T. aestivum L.). Iranian Journal of Soil Research, 13, 87-97. (in Farsi)
  33. Sarmadniya, A. & Kochaki, A. (2001). Grapes. In: R. Mishel & F. Pigardner, Crop Physiology. University of Mashhad, 45. (in Farsi)
  34. Saii, M. (2004). Evaluation of some morphological traits and drought tolerance of different Cultivars of Sorghum bicolor. M.Sc. thesis. Islamic Azad University of Karaj. (in Farsi)
  35. Tesfaye, K., Walker, S. & Tsubo, M. (2006). Radiation interception and radiation use efficiency of three grain legumes under water deficit conditions in a semi-arid environment. European Journal of Agronomy, 25(1), 60-70.
  36. Vanacker, H., Carver, T. L. W. & Foyer, C. H. (1998). Pathogen-Induced Changes in the Antioxidant Status of the Apoplast in Barley Leaves. Journal of Plant Physiology, 117(3), 1103-1114.
  37. Zeinali, A. & Soltani, A. (2002). Response of germination components to salinity stress in oilseed rape (Brassica napus L.). Journal of Agriculture Science, 36, 457-463. (in Farsi)



Volume 49, Issue 1
June 2018
Pages 11-27
  • Receive Date: 28 August 2016
  • Revise Date: 04 March 2017
  • Accept Date: 03 April 2017
  • Publish Date: 22 May 2018