Evaluation of salinity tolerance in two safflower cultivars using ions relations and biochemical traits

Document Type : Research Paper

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Abstract

To investigate salinity tolerance in two safflower cultivars and identify the most important traits affecting salinity tolerance in safflower cultivars (Carthamus tinctorius L.), a greenhouse experiment was conducted as factorial arrangement based on completely randomized design with four replications. Treatments were irrigation water salinity (2, 4, 8 and 12 dS/m) and safflower cultivars (Sofeh and Zendehrud). Plant dry weight in Sofeh cultivar decreased 13, 30 and 58% respectively, while, plant dry weight of Zendehrud cultivar reduced 9, 28 and 40% under salinity levels. The higher concentration of chlorophyll a, b, SPAD, peroxidase and superoxide dismutase activity was found in Zendehrud cultivar under all salinity levels. Enhancement of Na+ concentration in shoot and root, proline and malondialdehyde was greater in Sofeh cultivar compared to Zendehrud cultivar. Linear regression analysis revealed significant and negative relationships between plant dry weight with Na+ concentration in leaf and root and proline content. Plant dry weight was significantly and positively associated with K+/Na+ ratio, peroxidase, superoxide dismutase and chlorophyll a. In general, it was concluded that Na+ concentration in root, shoot (stem + leaf), peroxidase and superoxide dismutase activity can be used as reliable indicators in identifying salinity tolerant cultivars of safflower

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References

  1. Abdul-Jaleel, C., Gopi, R., Sankar, B., Manivannam, P., Kishorekumar, A., Sridharan, R. & Panneerselvan, R. (2007). Studies on germination, seedling vigour, lipid peroxidation and prolinemetabolism in Catharanthus roseus seedlings under salt stress. South African Journal of Botany, 73, 190-195.
  2. Agrawal, S., Sairam, R.K., Srivasta, G.C., Tyagi, A. & Meena, R.C. (2005). Role of ABA, salicylic acid, calcium and hydrogen peroxide on antioxidant enzymes induction in wheat seedling. Plant Science, 169, 559-570.
  3. Arzani, A. & Ashraf. M. (2016). Smart Engineering of Genetic Resources for Enhanced Salinity Tolerance in Crop Plants. Critical Review in Plant Science. 35(3): 146-189.
  4. Ashraf, M. & Ahmad, S. (2000). Influence of sodium chloride on ion accumulation, yield components and fiber characteristics in salt-tolerant and salt-sensitive lines of cotton (Gossypium hirsutum L.). Field Crops Research 66, 115-127.
  5. Ashraf, M. & Harris, P.J.C. (2004). Potential biochemical indicators of salinity tolerance in plants. Plant Science, 166, 3-16.
  6. Ashraf, M. & Ali, Q. (2008). Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napusL.). Environmental and Experimental Botany, 63, 266–273.
  7. Ashraf, M. & Foolad, M.R. (2007). Roles of Glycine Betaine and Proline in Improving Plant Abiotic Stress Resistance. Environmental and Experimental Botany, 59, 206-216.
  8. Ashraf, M., & Foolad, M. R. (2013). Crop breeding for salt tolerance in the era of molecular markers andmarker-assisted selection. Plant Breed. 132: 10–20.
  9. Bates, L., Waldren, R. & Teare, I. (1973). Rapid determination of free proline for water-stress studies. Plant and soil, 39: 205-207.
  10. Bazrafshan A.H. & Ehsanzadeh, P. (2014). Growth, photosynthesis and ion balance of sesame (SesamumindicumL.) genotypes in response to NaCl concentration in hydroponic solutions. Photosynthetica, 52(1), 134-147.
  11. Bowler, C., Slooten, L., Vandenbranden, S., De Rycke, R., Botterman, J., Sybesma, C., Van Montagu, M.,Inzé, D. (1991). Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. The EMBO Journal. 10(7), 1723-1732.
  12. Britton, C. & Mehley, A.(1955). Assay of catalase and peroxidase. Methods in Enzymology. 2, 764-775.13.
  13. Coskun, D., Britto, D.T., Jean, Y.K., Kabir, I., Tolay, I., Torun, A.A. & Kronzucker, J. (2013). K+ Efflux and retention in response to NaCl stress do not predict salt tolerance in contrasting genotypes of Rice (Oryza sativa L.). PLOS ONE. 8(2), 1-16.
  14. Dehindsa, R.S., Dehindsa, P.P. & Thorpe, T.A.(1981). Leaf Senescence: Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation, and Decreased Levels of Superoxide Dismutase and Catalase. Journal of  Experimental Botany. 32(1), 93-101.
  15. Farhoudi, R. (2011). Evolution effect of salt stress on growth, antioxidant enzymes activity and malondealdehaid concentration of Canola verities.  Iranian Journal of Field Crops Research, 9(1), 123-130. (In Farsi)
  16. Fidalgo, F., Santos, A., Santos, I. & Salema, R. (2004). Effects of long‐term salt stress on antioxidant defence systems, leaf water relations and chloroplast ultrastructure of potato plants. Annals of Applied Biology, 145, 185-192.
  17. Gao, S., Ouyang, C., Wang, S., Xu, Y., Tang, L. & Chen, F. (2008). Effects of salt stress on growth, antioxidant enzyme and phenylalanine and phenylalanine ammonia-lyase activities in Jatrophacurcas L. seedlings. Plant, Soil and Environment, 54,374-81.
  18. Garriga, M., Munoz, C.A., Caligari, P.D.S. & Retamales, J.B. (2015). Effect of salt stress on genotypes of commercial (Fragaria x ananassa) and chilean strawberry (F. chiloensis). Scientia Horticulture, 195, 37-47.
  19. Gengmao, Z., Yu, H., Xing, S., Shihui, L., Quanmei, S. & Changhai, W. (2015). Salinity Stress Increases Secondary Metabolites and Enzyme Activity in Safflower. Industrial Crops and Production, 64, 175-181.
  20. Gill, S. S. &  Tuteja, N. (2010). Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 48, 909-930.
  21. Gorgi, M., Zahedi, M. & Eshghizadeh, H.R. (2015).  Relationship between responses of Safflower genotypes to salinity at germination and vegetative growth stages. Journal of Crop Production and Processing, 4(14), 147-158.(In Farsi)
  22. Hafsi, C., Romero-Puertas, M.C., Gupta, D.K., Del-Rio, L.A., Sandalio, L.M. &Abdelly, C. (2010). Moderate salinity enhances the antioxidative response in the halophyte Hordeum maritimum L. under potassium deficiency. Environmental and Experimental Botany, 60, 129-136.
  23. Heath, R.L. & Packer, L. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics. 125, 189–198.
  24. Hu, L., Li, H., Pang, H. & Fu, J. (2012). Responses of antioxidant gene, protein and enzymes to salinity stress in two genotypes of perennial ryegrass (Lolium perenne) differing in salt tolerance. Journal of Plant Physiology, 169, 146-156.
  25. Javadipour, Z., Movahhedi Dehnavi, M. & Balouchi, H.R. (2013). Changes in the rate of proline, soluble sugars, glycinebetaine and protein content in leaves of six spring safflower (Carthamus tinctiriusL.) under salinity stress.  Journal of Plant Process and Function, 1(2), 13-24. (In Farsi)
  26. Javadipour, Z., Movahhedi Dehnavi, M. & Balouchi, H.R. (2013). Evaluation of photosynthesis parameters, chlorophyll content and fluorescence of safflower cultivars under saline condition. Electronic Journal of Crop Production, 6(2), 35-56. (In Farsi)
  27. Karimi, S., Arzani, A. & Saeidi, G. (2014). Differential response of ion and osmolyte accumulation to salinity stress in salt-tolerant and salt-sensitive seedlings of safflower (Carthamus tinctoriusL.). Research on Crops, 15,802-809.
  28. Kaya, C., Higges, D. &Kirnak, H. (2001). The effects of high salinity (NaCl) and supplementary phosphorus and potassium on physiology and nutrition development of spinach. Journal of Plant Physiology, 27, 47-59.
  29. Lacerda, C. F., Cambraia, J., Oliva, M.A. & Ruiz, H.A. (2005). Changes in growth and in solute concentrations in sorghum leaves and roots during salt stress recovery. Environmental and Experimental Botany, 54, 69-76.
  30. Lichtenthaler, H.K. (1987). Chlorophyll and carotenoids: pigments of photosynthetic biomembranes. Meth. Enzym, 148, 331-382.
  31. Mass, E.V. (1985). Crop tolerance to sprinkling water. Plant and Soil, 89, 273-284.
  32. Meloni, D. A., Oliva, M.A., Martinez, C.A. & Cambraia, J. (2003). Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Brazilian Journal of Plant Physiology, 15 (2), 12-21
  33. Munns, R. & Tester, M. (2008). Mechanisms of Salinity Tolerance. Annual Review of Plant Biology, 59, 651-681.
  34. Olsen, S. R. C., Cole, V., Watanabe, F.S. & Dean, L.A. (1954). Estimation of available phosphorus in soil by extraction with sodium bicarbonate. USDA. Circ.939. U. S. Gov Print. Office, Washington, D. C.
  35. Rahimi, A., Dashti, H. & Zibai, S. (2012). Effects of seed priming on some physiological traits of safflower (Carthamus tinctorius L., cv. Goldasht) under salinity stress. Journal of Crop Production and Processing, 2(3), 1-15.(In Farsi)
  36. Salem, N., Msaada, K., Dhifi, W., Limam, F. & Marzouk, B. (2014). Effect of salinity on plant growth and biological activities of Carthamus tinctorius L. extracts at two flowering stages. Acta Physiologiae Plantarum, 36, 433-445.
  37. Sarvajeet, S. G. & Narendra, T. (2010). Reactive oxygen species and antioxidant machinery in a biotic stress tolerance in crop plants. Plant Physiology and Biochemistry, 3, 1-22.
  38. Sheidaie S.,Zahedi, M. & Mirmohammadi Meibodi, A.M. (2010). Effect of salinity on dry matter accumulation and ion distribution of safflower genotypes. Iranian Journal of Field Crop Science, 4, 811-819. (In Farsi).
  39. Siddiqi, E.H. & Ashraf, M. (2008). Can leaf water relation parameters be used as selection criteria for salt tolerance in safflower (Carthamus tinctorius L.). Pakistan Journal of Botany, 40, 221-228.
  40. Tavakoli, F., Vazan, S., Sorkheh, K. and Shakeri, E. (2016).  Effect of salinity stress on some physiological traits and electrophoresis pattern of leaf proteins of two Barley genotypes.  Journal of Crop Production and Processing, 6(19), 191-201. (In Farsi)
  41. Wu, H., Shabala, L., Zhou, M., Stefano, G., Pandolfi, C., Mancuso, S., & Shabala, S. (2015). Developing and validating a high-throughput assay for salinity tissue tolerance in wheat and barley. Planta 242, 847-857.
  42. Vijayalakshmi, T., Vijayakumar, A.S., Kiranmai, K., Nareshkumar, A. & Sudhakar, C. (2016). Salt stress induced modulations in growth, compatible solutes and antioxidant enzymes response in two cultivars of safflower (Carthamus tinctorius L. cultivar TSF1 and cultivar SM differing in salt tolerance. American Journal of Plant Sciences. 7, 1802-1819.
  43. Yeilaghi, H., Arzani, A., Ghaderian, M., Fotovat, R., Feizi, M. & Pourdad, S.S. (2012). Effect of salinity on seed oil content and fatty acid composition of safflower (Carthamus tinctorius L.) genotypes. Food Chemistry, 130, 618-625.
  44. Yeilaghi, H., Arzani, A., Ghaderian, M. (2015). Evaluating the contribution of ionic and agronomic components toward salinity tolerance in safflower. Agronomy Journal, 107 (6), 2205-2212.
  45. Zibaei, S., Rahimi, A. & Dashti, H. (2012). Effects of seed priming on growth, chlorophyll content, relative water content and dry matter distribution of Safflower (Carthamus tinctorius, cv. Gholdasht) under salinity stress. Journal of Crop Production and Processing, 2(5), 47-58. (In Farsi)
Iranian Journal of Field Crop Science
Volume 48, Issue 4 - Serial Number 4
March 2018
Pages 1211-1225

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History

  • Receive Date: 31 January 2017
  • Revise Date: 13 May 2017
  • Accept Date: 22 May 2017
  • Publish Date: 20 February 2018

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