Assessment of Genetic Variation in Silybum marianum L. Using ISSR Markers

Document Type : Research Paper

Authors

1 PhD student in Agronomy Department, College of Agriculture and Natural Resources, University of Tehran.

2 Professor of University of Tehran

3 Research Center of Zanjan

4 Department of Agronomy, College of Agriculture and Natural Resources, University of Tehran

Abstract

To study the genetic diversity in 77 samples of Silybum marianum (consisting 21 ecotypes) were evaluated in a randomized complete block design with three replications in Agricultural site of University of Zanjan. Using ISSR markers, the genetic diversity of some ecotypes of Silybum marianum along with their classification were studied. Out of 12 ISSR primers, 10 were amplified. Results revealed that the highest polymorphism information content (PIC) value belongs to primers UBC809 (0.492), UBC842 (0.487), and UBC826 (0.443 (, and lowest value was that of primer UBC1 (0.121). Cluster analysis using UPGMA algorithm divided the lines into 9 separate groups. For molecular analysis of variance of ecotypes a primary classification based on the geographical area used which divided them into 8 groups. Results of molecular analysis of variance revealed that within population variance was higher than between group variance due to its open pollinating nature. Regarding to principal component analysis, the first six components explained 87.5 % of the total variance and each component accounts for a small percentage of the total variance that emphasizes on good genomic distribution of selected ISSR markers. In general, the results obtained for using ISSR markers showed that this technique is a suitable one to study the genetic structure of the population Silybum marianum

Keywords

Main Subjects

References

  1. Ahmadi, H., Rahimmalek, M. & Zeinali, H,. (2014). Assessment of the genetic variation of chamomile (Matricaria chamomilla L.) populations using phytochemical, morphological and ISSR markers. Biochemical Systematics and Ecolog. Vol 54. Pp 190-197.
  2. Aminjorabadi, F., Cheghamirza, K. & Bahraminejad, S. (2012). Assessment of Genetic Variation in Silybum marianum Using Mollecular Markers, 12 th Iranian Genetics Congress, Sh Beheshti University.(In Farsi)
  3. Asghari-Zakaria, R., Kazemi, H., Aghayev, Y.M., Valizadeh, M. & Moghaddam, M., (2002). Karyotype and C-banding patterns of mitotic chromosomes in Henrardia persica (Boiss.) C.E. Hubb. Caryologia 55(4): 289-293.
  4. Azdet, T., Iglesias, J., & Martinez, F., (1993). Flavnolignans in the fruits of Silybum genus taxa: A chromatographic and mass spectrometric survey. Plants Medicianales. 26 :117- 129
  5. Bramwell, D. (2002). How many plant species are there? Plant Talk Science. 28: 32–34.
  6. Chen, J., Gituru, W., Wang, Y. & Wang, Q. (2006). The extent of clonality and genetic diversity in the rare Caldesia grandis (Alismataceae): comparative results for RAPD and ISSR markers. Aquatic  Botany 27: 45-52.
  7. Fazeli, F & Cheghamirza, K . (2011). Investigation of genetic diversity in iranian landrace chickpea bulks using ISSR marker. Modern genetic, vol6(2), 97-104.
  8. Garayalde, A. F., Poveren, M. & Carrera, A. D. (2011). Wild sunflower diversity in Argentina reveald by ISSR and SSR markers an approach for conservation and breeding programmers. Annals of Applied Biology 158: 305-317.
  9. Gahreman, A. (1983). Collered Flore of Iran. Research Institute of Forests and Rangelands. 9: 1095-1099.  
  10. Gharibi, Sh., Rahimmalek, M., Mirlohi, A. & Majidi, M. (2011). Assessment of genetic diversity in Achillea millefolium subsp. Illefolium and Achillea illefolium subsp. Elbursensis using morphological and ISSR markers . Jornal Medici Research vol .5 (11): 2413-2423.
  11. Golkar , P., Arzani , A. & Abdolmajid, R. (2011). Genetic Variation in Safflower (Carthamus tinctorious L.) for Seed Quality-Related Traits and Inter-Simple Sequence Repeat (ISSR) Marker. Molecular Sciences. 12: 2664-2677.
  12. Hamrick, J.L. & Godt, M.J.W. (1997). Effects of life history traits on genetic diversity in plant species. 102-118. In: Silvertown J., (ed.). Plant LifeHistories. Ecology, Phylogeny and Evolution.Cambridge University Press, Cambridge, UK. 307.
  13. Hatami Maleki, H., Darvishzadeh, R. & Mohseni, Z., (2014). Evalution of genetic diversity and classification of advanced sun flower lines using ISSR markers, Agricultural Biotechnology, 6(3), 33-45. .(In Farsi)
  14. Krasnikov, A.A., Zhirova, O.S., Lomonosova, M.N. & Smirnov, S.V. (2003). Chromosome numbers of Asteraceae from the southern Siberia and Kazakstan. Euphytica. 88: 151–153.
  15. Kren V, Ulrichova J, Kosina P, Stevenson D, Sedmera P, Prikrylova V, Halada P. & Simanek V.,( 2000). Chemoenzymatic preparation of silybin β- glucoronides and their biological evaluation. Drag Metabolism and Disposition.; 28: 1513-1517.
  16. Lopez-Pujol, J., Bosch, M., Simon, J. & Blanche, C. (2004). Allozyme diversity in the tetraploid endemic Thymus loscosii (laminaceae). Annals of Botany, 93,0323-332.
  17. 17. Mohammadi,  S.,   Shokrpour , M., Moghaddam, M. & Javanshir , A.,( 2011). AFLP-based molecular characterization and population structure analysis of Silybum marianum L. Plant Genetic Resources 9 (3) : 445-453.
  18. Mohammadi,, S.A. & Prassanna, B. M. (2003). Analysis of genetic diversity in crop plants – salient statistical tools and considerations. Crop Science, 43, 1235-1248.
  19. Moraga, A., Trapero-Mozos, A., Gómez-Gómez, L. & Ahrazem, O. ,(2010). Intersimple sequence repeat markers for molecular characterization of Crocus cartwrightianus cv. albus. Ind. Crop Science. 123–132 .
  20. Powell, W., Morgante, M., Andre, C., Vgel, J., Tingey, S., and Rafalaski, A. (1996). The comparison of RFLP, RAPD, AFLP and SSR (microsatelite) markers for germplasm analysis. Molecular Breeding, 2, 225-238.
  21. Rainone , F.,( 2005). Milk thistle. American Family Physician; 72(7): 1285-1288
  22. Ram, G. Bhan, M.K. Gupta, K.K. Brijesh Thaker,U. Jamwal, S. Pal. 2005. Variability pattern and correlation studies in Silybum marianum Gaertn. Fitoterapia. 76: 143-147.
  23. Rasmieh, H., Siahposh, M., Mameghani, R. & Siahposh, M.R. (2014). Assessment of Genetic Variation in ten Ecotype Silybum marianum Using Morphologic, phenologic and phytochemical characters,Plant Production technology, 1(37), 35-45 .(In Farsi)
  24. Sepehry Javan, Z., Rahmani,  F. & Heidari, R.,( 2012).  Assessment of genetic variation of genus Salvia by RAPD and ISSR markers. Australian Journal of Crop Science, 6(6):1068-1073.
  25. Sharaf1. A., Ahmed Bahieldin. A.,  Ibrahim. S . &  Khalil, A.( 2010). Biochemical and genetic characterization of 12 Silybum marianum accessions collected from Borg El-Arab, an Egyptian Habitat, Functional Plant Science and Biotechnolog. 12 (5) : 22-27
  26. Shokrpour, M., Mohammadi, S.A., Moghaddam, M., Ziai, S.A. & Javanshir, A.( 2008). Variation in flavonolignan concentration of milk thistle (Silybum marianum) fruits grown in Iran. Journal of Herbs, Spices & Medicinal Plants( In Farsi).
  27. Wang, X., Zhao, F., Hu, Z. & Critchley, A. (2008). Inter-simple sequence repeat (ISSR) analysis of genetic variation of Chondrus crispus populations from North Atlantic. Aquatib Botany.31: 145-154.
Iranian Journal of Field Crop Science
Volume 48, Issue 4 - Serial Number 4
March 2018
Pages 1115-1124

Files

History

  • Receive Date: 22 January 2017
  • Revise Date: 31 March 2017
  • Accept Date: 12 April 2017
  • Publish Date: 20 February 2018

Share

How to cite

Statistics