Effect of water deficit stress on some physiological and biochemical traits of forage sorghum genotypes

Document Type : Research Paper

Abstract

This study was performed to investigate the effect of water deficit stress on the some activities antioxidant enzymes, photosynthetic pigments and forage yield of sorghum genotypes. This experiment was conducted to factorial based on completely randomized design with three replications in greenhouse of University of Mohaghegh Ardabili in 2013. Treatments consisted of irrigation at four levels (85, 65, 45 and 25% of field capacity) and three genotypes of forage sorghum (KFS2, KFS6, and KFS17). Catalase, peroxidase and polyphenol oxidase enzymes and soluble sugar content, proline and carotenoids, increased in severe stress (25% field capacity) and decreased chlorophyll a and chlorophyll b. KFS6 genotype had the highest chlorophyll a, chlorophyll b, and total chlorophyll, however, the maximum biomass, proline, soluble sugars, peroxidase and polyphenol oxidase activity and carotenoid were observed by KFS2 genotype. Also positive correlation were observed between the biomass production and carotenoid and chlorophyll content, proline, soluble sugar, catalase, peroxidase and negative correlation between biomass and polyphenol oxidase. Between photosynthetic indices, chlorophyll a, between adjustment indices, peroxidase activity had maximum contribution to prediction biomass. Also in KFS2 genotype except carotenoid and proline all traits had highest correlation with biomass. In general KFS2 genotypes introduced tolerant to water stress as compared with the other genotypes.

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References

  1. Abbas-Zade, B., Sharifi. A., Abadi, A., Lebaschi, M.H., Naderi, M. & Maghdami, F. (2007). Effect of drought stress on proline, soluble sugars, chlorophyll and relative water content of Melissa officinalis L. Journal Research Agronomic Plants Iran, 23, 504-513.
  2. Abdelgawad, Z. A. Khalafaallah, A. A. & Abdallah M. M. (2014). Impact of Methyl Jasmonate on Antioxidant Activity and Some Biochemical Aspects of Maize Plant Grown under Water Stress Condition. Agricultural Sciences,  5,1077-1088
  3. Ariano, S., Bartolomeo, D., Cristos, X. & Andras, M. (2005). Antioxidant defenses in Olive trees during drought stress: changes in activity of some antioxidant enzymes. Functional Plant Biology, 32, 45- 53.
  4. Arnon, A.N. (1967).Method of extraction of chlorophyll in the plants. Agronomy Journal, 23, 112- 121.
  5. Bajji, M., Lutts, S. & Kinet, J.M. (2001). Water deficit effects on solute contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. Plant Science, 160, 669- 681.
  6. Bates, I.S., Waldern, R.P. & Teare, I.D. (1973). Rapid determination of free Proline for water stress studies. Plant and Soil, 39, 205- 207.
  7. Bessembinder, J.J.E., Leffelaar, P.A., Dhindwal, A.S. & Ponsioen, T.C. (2005). Which crop and which drop, and the scope for improvement of water productivity. Agricultural Water Management, 73, 113- 130.
  8. Bradford, M.M. (1976).A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemist,72, 248-254
  9. Cattivelli, L., Rizza, F., Badeck, F.W., Mazzucotelli, E., Mastrangelo, A.M., Francia, E., Mare, C., Tondelliand A. & Stanca M.(2008). Drought tolerance improvement in crop plants: An integrated view from breeding to genomics. Field Crops Research, 105, 1-14.
  10. Dehqanzadeh, H., Khajehpour, M.R., Heidari Sharif Abad, H. & Soleimani, A.S. (2008).Effect of limited irrigation on the accumulation of proline, free soluble sugars and potassium in bread wheat cultivars. 10th Iran. Cong Agron. Plant Breed Science, 430p.
  11. Devnarain, N, Crampton, BG, Chikwamba, R, Becker, JVW. & O’Kennedy, MM. (2016). Physiological responses of selected African sorghum landraces to progressive water stress and re-watering. The South African Journal of Botany, 103, 61-69.
  12. Foyer, C. & Noctor, G. (2003). Redox sensing and signaling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. PhysiologyPlanetarium, 119, 355- 364.
  13. Garg, N. & Manchanda, G. (2009). ROS generation in plants: boon or bane. Plant Biopsy, 143, 8- 96.
  14. Ghorbanli, M. & Niakan, M. (2005). Effect of drought stress on soluble sugar, protein, proline, phenolic compounds and nitrate reductase enzyme activity in soybean varieties in Gorgan. Journal of Science Teacher Education, 5, 537-549. (In Farsi).
  15. Gunes, A., Inal, A., Adak, M.S., Bagci, E.G., Cicek, N. & Eraslan, F. (2008). Effect of drought stress implemented at pre-or post-anthesis stage on some physiological parameters as screening criteria in chickpea cultivars. Russian Journal Plant Physiology, 55, 59- 67.
  16. Haj Hosseini Asl, N., Moradi Aghdam, A., Shirani Rad, A.H., Hosseini, N. & Resay Far, M. (2010). Effects of drying stress on yield and agronomic traits of millet, sorghum and maize relay intercropping. Journal of Agricultural Research, 1, 63-74. (In Farsi)
  17. Hassanzadeh, M., Ebadi, A., Panahyan-e-Kivi, M., Eshgih, A.G., Jamaati-e-Somarin, Sh., Saeidi, M. & Zabihie Mahmoodabad, R. (2009). Evaluation of drought stress on relative water content and chlorophyll content of Sesame (Sesamum indicum L.) genotypes at early flowering stage. Research Journal of Environmental Sciences,3(3), 345-350.
  18. Ingram, J. & Bartles, D. (1996).The molecular basis of dehydration tolerance in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 47, 377- 403.
  19. Irigoyen, J.J., Emerich, D.W. & Sanchez- Diaz, M. (1992). Alfalfa leaf senescense induced by drought stress: photosnthesis, hydrogen peroxide metabolism, lipid peroxidation and athylene evoluation. Physiology Planetarium, 84, 67- 72.
  20. Jabari, f., Ahmadi, A., Poustini, K. & Alizadeh, H. (2006). Evaluation of some antioxidant enzyme effects on chlorophyll and cell membrane in drought susceptible and tolerant wheat varieties. Iranian Journal Agriculture Science,37, 307-316.
  21. Karo, M. & Mishra, D. (1976). Catalase, peroxidase and polyphenol oxidase activity during rice leaf senescence. Plant Physiology 57: 315-319.
  22. Koyro, H.W. (2006). Effect of salinity on growth, photosynthesis, water relations and solute composition of potential cash crop halophyte Plantago coronopus (L.). Environmental and Experimental Botany, 56, 136-149.
  23. Liu, H., Sultan, M.A.R.F., Liu, X.L., Zhang, J., Yu, F. & Zhao, H.X., (2015). Physiological and comparative proteomic analysis reveals different drought responses in roots and leaves of drought-tolerant wild wheat (Triticum boeoticum). PLoS One, 10(10), 25-36.
  24. Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends in Plant Science, 7, 405-410.
  25. Mittler, R., Vanderauwera, S., Gollery, M. & Breusegem, F.V. (2004). Reactive oxygen gene network of plants. Trends in Plant Science, 9, 490- 498.
  26. Mohsenzade, S., Malboobi, M.A., Razavi, K., & Farrahi Aschtiani, S. (2006). Physiological and molecular responses of Aeluropus lagopoides (poaceas) to water deficit. Environmental and Experimental Botany, 56, 374-322.
  27. Movludi, A. Ebadi, A. Jahanbakhsh, S. Davari, M. & Parmoon. Gh. (2014). The effect of water deficit and nitrogen on the antioxidant enzymes’ activity and quantum yield of barley (Hordeum vulgare L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 42(2), 398-404.
  28. Mureiel, J. (1984). Free proline and reducing sugars accumulation in water stress. Ser-Agrincola, 29, 39- 46.
  29. Oncel, I., Keles, Y. & Ustun, A.S. (2000). Interactive of temperature and heavy metal stress on the growth and some biological compounds in wheat seedling. Environmental Pollution, 107, 15-320.
  30. Pagter, M., Bragato, C. & Brix, H. (2005). Tolerance and physiological responses of phragmites australis to water deficit. Aquatic Botany, 81, 285-299.
  31. Polidoros, N.A. & Scandalios, J.G. (1999). Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferees gene expression in maize (Zea mays L.). Physiology Planetarium, 106, 112- 120.
  32. Ranjan, R., Bohra, S.P. & Jeel, A.M. (2001). Book of plant senescence. Jodhpur, Agrobios New York, PP. 18- 42.
  33. Riahani Nia, SH., Khazaei H. R. Kafie, M. & Nezami, A. (2012). Effect of soil drought stress and different nitrogen levels on some biochemical characteristics of grain sorghum cultivars in greenhouse conditions. Journal of Science and Technology of Greenhouse Culture, 70, 14-60. (In Farsi).
  34. Sajjadinia, A., Ershadi, A., Hokmabadi, H., Khayyat, M. & Gholami, M. (2010). Gas exchange activities and relative water content at different fruit growth and developmental stages of on and off cultivated pistachio trees. American Journal of Agricultural Economics, 1, 1- 6.
  35. Sudhakar, C., Lakshmi, A. & Giridara kumar, S. (2001). Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Mours alba L.) under NaCl salinity, Plant Science, 167, 613-619.
  36. Turkan, I., Bor, M., Ozdemir, F. & Koca, H. (2005). Differential responses of lipid peroxidation and antioxidants in the leaves of drought tolerant P. acutifolius Gray and drought sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science, 168, 223- 231
  37. Umar, SH. (2006).Alleviating adverse effects of water stress on yield of sorghum, mustard and groundnut by potassium. Pakistan Journal of Botany, 38, 1373-1380.
Iranian Journal of Field Crop Science
Volume 48, Issue 4 - Serial Number 4
March 2018
Pages 1083-1097

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History

  • Receive Date: 21 November 2015
  • Revise Date: 20 February 2018
  • Accept Date: 31 May 2017
  • Publish Date: 20 February 2018

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