Investigation of lim, medh gene expression level and morpho-physiological changes of Mentha piperiat L. in response to drought

Document Type : Research Paper

Authors

1 Ph.D. Candidate, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Professor, Department of Agronomy and Plant Breeding, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

3 Assistant Professor, Amol University of Special Modern Technologies, Amol, Iran

Abstract

Peppermint known as important medicinal plants that used in the food, medical and pharmaceutical industry. In present study the effect of drought stress at four levels: 100 (as control), 75, 50 and 25% field capacity (FC) were investigated on expression of limonene synthase (lim) and menthol dehydrogenase (mdeh) genes in menthol pathway as well as morpho-physiological responses and performance of peppermint essential oil was evaluated using GC-MS analysis. Results showed that drought stress effect on fresh and dry weight of shoot, leaf area, leaf number, proline content and relative water content (RWC) significantly (P˂0.01) differences. Drought stress caused a significant decrease in the morphological traits also the proline content and relative water content increased and declined respectively. Essential oil yield increased under drought stress condition, although the increase was not significant. The relative expression of lim gene increase at 75 and 50­% FC, while showed a significant decreased at 25% FC. The relative expression of mdeh gene decreased at three regime of irrigation compared to control, significantly. In general, drought stress increased oil yield but its quality was declined.

Keywords

Main Subjects

References

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Iranian Journal of Field Crop Science
Volume 48, Issue 2 - Serial Number 2
September 2017
Pages 507-516

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History

  • Receive Date: 19 October 2016
  • Revise Date: 03 January 2017
  • Accept Date: 20 February 2017
  • Publish Date: 23 August 2017

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