Effect of hydropriming on morphological and physiological performance of aged groundnut (Arachis hypogaea L.) seeds

Document Type : Research Paper


1 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

2 Ph.D. Candidate, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran


Low viability and poor vigour due to seed deterioration are major problems for successful agricultural production. In this experiment, the ability of hydropriming to ameliorate seed deterioration damage was studied in groundnut. Groundnut seeds were subjected to accelerated ageing for 96 hours at 40 °C and then hydroprimed at 25 °C for 6, 12 and 18 hours. Regardless of duration, hydropriming significantly improved final germination percentage, germination rate, seedling length, vigour index, antioxidant enzyme activities (catalase, superoxide dismutase and ascorbate peroxidase),soluble sugars and proteins of aged seeds. Mean germination time, electrolyte leakage and malondialdehyde content of primed seeds decreased compared to non-primed seeds. Hydropriming for 6, 12 and 18 hours increased final germination to 15.54, 31.56 and 89.7 %, germination rate by 9.42, 66.6 and 95.6 % and vigour index by 29.11, 82.2 and 204.6 % compared to non-primed seed, respectively. Thus, hydropriming for 18 hours is the most suitable priming period to recover loss of seed quality and to improve germination characteristics of aged groundnut seeds.


Main Subjects

  1. Abdulrahmani, B., Ghasemi-Golezani, K., Valizadeh, M. & Feizi-Asl, V. (2007). Seed priming and seedling establishment of barley (Hordeum vulgare L.). Journal of Food Agriculture and and Environment, 5, 179-184.
  2. Ahmad, G. & Lee, H. C. (2011). Response of sesame (Sesamum indicum) cultivars to hydropriming of seeds. Australian Journal of Basic Applied Science, 1,638-642.
  3. Ahmad, K. U., Rahman, M. M. & Ali, M. R. (2014).Effect of hydropriming method on maize (Zea mays) seedling emergence. Bangladesh Journal of Agriculture Research, 39(1), 143-150.
  4. Ashraf, M. & Foolad, M. R. (2005). Pre-sowing seed treatment a shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy, 88, 223-271.
  5. Bailly, C. (2004). Active oxygen species and antioxidants in seed biology. Seed Science Research, 14, 93-107.
  6. Bradford, M. M. (1976). A dye binding assay for protein. Analytical Biochemistry, 72, 248-254.
  7. Capron, I., Corbineau, F., Dacher, F., Job, C., Come, D. & Job, D. (2000). Sugarbeet seed priming: Effects of priming conditions on germination, solubilization of 11‐S globulin and accumulation of LEA proteins. Seed Science Research, 10, 243-254.
  8. Caseiro, R., Bennett, M. A. & Marcos-Filho, J. (2004). Comparison of three priming techniques for onion seed lots differing in initial seed quality. Seed Science and Technology, 32, 365-375.
  9. Cakmak, I. & Horst, W. (1991). Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glycine max). Plant Physiology, 83, 463-468.
  10. Cavalcanti, F. R., Oliveira, J. T. A., Martins-Miranda, A. S., Viégas, R. A. & Silveira, J. A. G. (2004). Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpeas leaves. New Phytologist, 163, 563-571.
  11. Chiu, K. Y., Wang, C. S. & Sung, J. M. (1995). Lipid peroxidation and peroxide-scavenging enzymes associated with accelerated ageing and hydration of watermelon seeds differing in ploidy. Plant Physiology, 94, 441-446.
  12. Delouche, J. C. & Baskin, C. C. (1973). Accelerated ageing technique for predicting relative storability of seed lots. Seed Science and Technology, 1, 427-452.
  13. El-Araby, M. M. & Hegazi, A. Z. (2004). Responses of tomato seeds to hydro-and osmopriming, and possible relations of some antioxidant enzymes and endogenous polyamine fractions. Egyptian Journal of Biology, 6, 81-93.
  14. Ellis, R. A. & Roberts, E. H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9, 373-409.
  15. Fujikura, Y., Kraak, H. L., Basra, A. S. & Karssen, C. M. (1993). Hydropriming, a simple and inexpensive priming method. Seed Science and Technology, 21, 639-642.
  16. Ghasemi-Golezani, K., Aliloo, A. A., Valizadeh, M. & Moghaddam, M. (2008). Effects of different priming techniques on seed invigoration and seedling establishment of lentil (Lens culinaris Medik.). Journal of Food Agriculture and and Environment, 6, 222-226.
  17. Ghasemi-Golezani, K., Chadordooz-Jeddi, A., Nasrollahzade, S. & Moghaddam, M. (2010). Effects of hydro-priming duration on seedling vigour and grain yield of pinto bean (Phaseolus vulgaris L.) cultivars. Notulae Botanicae Horti Agrobotanici, 38(1), 109-113.
  18. Ghasemi-Golezani, K., Japparpour-Bonyadi, Z., Shafagh-Kolvanagh, J. & Nikpour-Rashidabad, N. (2013). Effects of water stress and hydro-priming duration on field performance of lentil. International Journal of Farming and Allied Science, 2, 922-925.
  19. Giannopolitis, C. & Ries, S. (1977). Superoxid desmutase. I: Occurence in higher plant. Plant Physiology, 59, 309-314.
  20. Hampton, J. G. & TeKrony, D. M. (1995). Handbook of Vigour Test Methods. The international Seed Testing Association, Zurikh.
  21. Hsu, C. C., Chen, C. L., Chen, J. J. & Sung, J. M. (2003). Accelerated ageing-enhanced lipid peroxidation in bitter gourd seeds and effects of priming and hot water soaking treatments. Horticultural Science, 98, 201-212.
  22. Irigoyen, J. J., Emerich, D. W. & Sanchez-Diaz, M. (1992). Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84, 55-60.
  23. Jisha, K. C., Vijayakumari, K. & Puthur, J. T. (2013). Seed priming for abiotic stress tolerance: an overview. Acta Physiologiae Plantarum, 35, 1381-1396.
  24. Jyotsna, V. & Srivastava, A.K. (1998). Physiological basis of salt stress resistance in pigeonpea (Cajanus cajan L.)-II. Pre-sowing seed soaking treatment in regulating early seedling metabolism during seed germination. Plant Physiology and Biochemistry, 25, 89-94.
  25. Kamithi, K. D., Wachira, F. & Kibe, A. M. (2016). Effects of different priming methods and priming durations on enzyme activities in germinating chickpea (Cicer arietinum L.). American Journal of Natural and Applied Sciences, 1(1), 1-9.
  26. Kaya, M. D., Gamze, O., Atal, M., Yakup, C. & Ozer, K. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). Europian Journal of Agronomy, 24, 291-295.
  27. Kibinza, S., Vinel, D., Côme, D., Bailly, C. & Corbineau, F. (2006). Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologica Plantarum, 128, 496-506.
  28. Kibinza, S., Bazin, J., Bailly, C., Farrant, J. M., Corbineau, F. & El-Marrouf Bouteau, H. (2011). Catalase is a key enzyme in seed recovery from ageing during priming. Plant Science, 181, 309-315.
  29. Langens-Grrits, M. M., Miller, W. B., Croes, A. F. & De Klerk, G. J. (2003). Effects of low temperature on dormancy breaking and growth after planting in lily bulblets regenerated in vitro. Journal of Plant Growth Regulator, 40, 267-275.
  30. López, L. V. P., Rodríguez, A. R., Coronado, M. E. S., Hernández, P. E. M., Segovia, A. O. (2016). Effects of hydropriming treatments on the invigoration of aged Dodonaea viscosa seeds and water-holding polymer on the improvement of seedling growth in a lava field. Restoration Ecology, 24(1), 61-70.
  31. Matsushima, K. & Sakagami, J. (2012). Effects of seed hydropriming on germination and seedling vigor during emergence of rice under different soil moisture conditions. American Journal of Plant Science, 4, 1584-1593.
  32. Nakano, Y. & Asada, K. (1981). Hydrogen peroxide scavenged by ascrobate-specific peroxidase in spinach chloroplast. Plant Cell Physiology, 22, 867-880.
  33. Nascimento, W.M. (2012). Muskmelon seed germination and seedling development in response to seed priming. Scientia Agricola, 60(1), 71-75.
  34. Rahnama-Ghahfarokhi, A. & Tavakkol-Afshari, R. (2007). Methods for dormancy breaking and germination of galbanum seeds (Ferula gummosa). Asian Journal of Plant Science, 6, 611-616.
  35. Sacala, E. & Demczuk, A. (2016). Effect of laser and hydropriming of seeds on some physiological parameters in sugar beet. Journal of Elementology, 21(2), 527-538.
  36. Sallam, H. A. (1999). Effect of some seed-soaking treatments on growth and chemical components on faba bean plants under saline conditions. Annals of Agricultural Sciences, 44, 159-171.
  37. Sepehri, A. & Rouhi, H. R. (2016). Enhancement of seed vigor performance in aged groundnut (Arachis hypogaea L.) seeds by sodium nitroprusside under drought stress. The Philippine Agricultural Scientist, 99 (4), 339-347.
  38. Sharma, A. D., Rathore, S. V. S., Srinivasan, K. & Tyagi, R. K. (2014). Comparison of various seed priming methods for seed germination, seedling vigour and fruit yield in okra (Abelmoschus esculentus L. Moench). Scientia Horticulture, 165, 75-81.
  39. Sung, J. M. & Chiu, K. Y. (1995). Hydration effects on seedling emergence strength of watermelon seed differing in ploidy. Plant Science, 110, 21-26.
  40. Wattanakulpakin, P., Photchanachai, S., Ratanakhanokchai, K., Kyu, K. L., Ritthichai, P. & Miyagawa, S. (2012). Hydropriming effects on carbohydrate metabolism, antioxidant enzyme activity and seed vigor of maize (Zea mays L.). African Journal of Biotechnology, 11, 3537-3547.
  41. Wilson, D. O. & McDonald, M. B. (1986). The lipid peroxidation model of seed deterioration. Seed Science and Technology, 14, 269-300.
  42. Yan, M. (2015). Hydropriming promotes germination of aged napa cabbage seeds. Seed Science and Technology, 43(2), 303-307.
  43. Yan, M. (2016). Hydro-priming increases seed germination and early seedling growth in two cultivars of Napa cabbage (Brassica rapa subsp. pekinensis) grown under salt stress. The Journal of Horticultural Science & Biotechnology, 91(4), 1-6.
Volume 48, Special Issue
October 2017
Pages 43-53
  • Receive Date: 08 February 2016
  • Revise Date: 02 August 2016
  • Accept Date: 12 November 2016
  • Publish Date: 23 September 2017