Effects of different auxin solutions on rooting of Alfalfa (Medicago sativa L.) cuttings

Document Type : Research Paper


1 Ph.D Candidate, Department of Plant Breeding, Tarbiat Modares University, Tehran, Iran

2 Associate Professor, Department of Plant Breeding, Tarbiat Modares University, Tehran, Iran

3 Professor, Seed and Plant Improvement Research Institute, Karaj, Iran


Alfalfa is a cross-pollinating forage crops, so that maintenance and multiplication of selected plant material from breeding programs through seed is impossible. Vegetative propagation of selected plants through cutting allows rapid and reliable multiplication of alfalfa clones. This study examined the root development of three ecotypes of alfalfa cuttings after dipping in two auxin’s growth regulators, IAA (Indole acetic acid) and IBA (indole butyric acid) different solutions include 0, 25, 50, 100, 200 and 400 mgl-1 for 8, 16 and 24 hours. The study was performed in a split split split design with three replications. The results indicated that untreated cuttings with regulators produced no roots, while, all auxin treatments stimulated rooting on almost cuttings. Dipping treatments in 100mgl-1 of auxin solutions for 16 hour produced better root development than others. Among studied alfalfa ecotypes, Nikshahri showed highest rooting development. This method is more rapid, efficient and cost-effective than other vegetative reproduction methods in alfalfa which can be utilized in breeding programs such as producing synthetic and hybrid varieties and also maintenance and reproduction of different selfing generations in alfalfa.


Main Subjects

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Volume 48, Issue 2 - Serial Number 2
September 2017
Pages 535-543
  • Receive Date: 02 May 2016
  • Revise Date: 01 August 2016
  • Accept Date: 05 November 2016
  • Publish Date: 23 August 2017