Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Molecular phylogeny of the A genome using internal transcribed sequences (ITS) of ribosomal genes in some Triticum L. species

Document Type : Research Paper

Authors
Zeinab Safari 1
Ali-Ashraf Mehrabi 2
1 Ph.D. Candidate, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran
10.22059/ijfcs.2017.206594.654111
Abstract
The genus Triticum L. includes bread wheat and other important cultivated species, which are economically important for large parts of the human food. In this study, we conducted a phylogenetic analysis of A genome-possessing species of genus Triticum L. (T. aestivum, T. turgidum, T. urartu and T. boeticum). Here, the internal transcribed sequences (ITS) of nuclear ribosomal DNA were amplified by two pairs of primers in 26 genotypes from the above species. Sequenced amplicons were aligned by ClustalW. Divergence matrices and phylogenic dendrogram were made by MEGA 5.0. Results revealed the full length of sequences of ITS1 and ITS2 were 650 bp and 700bp, respectively and G+C content were 60.25 and 60.50 in them. High levels of conservation in sequences were found among genotypes (%63 and %88). Phylogenetic analysis using amplified sequences were successfully divided diploid and polyploid wheats into individual groups. Regarding to the results, there were close relationships within T. aestivum and T. turgidum and also within T. urartu and T. boeticum. However, our analysis suggests that the ITS molecular markers seem to be proper tools for plant phylogenetic studies.
Keywords
A genome
dendrogram
molecular phylogeny
ribosomal DNA
Subjects
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Iranian Journal of Field Crop Science
Volume 48, Issue 2 - Serial Number 2
Summer 2017
Pages 483-491

  • Receive Date 11 May 2016
  • Revise Date 07 August 2016
  • Accept Date 09 August 2016
  • Publish Date 23 August 2017