Molecular phylogeny of the A genome using internal transcribed sequences (ITS) of ribosomal genes in some Triticum L. species

Document Type : Research Paper


1 Ph.D. Candidate, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran

2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ilam University, Ilam, Iran


The genus Triticum L. includes bread wheat and other important cultivated species, which are economically important for large parts of the human food. In this study, we conducted a phylogenetic analysis of A genome-possessing species of genus Triticum L. (T. aestivum, T. turgidum, T. urartu and T. boeticum). Here, the internal transcribed sequences (ITS) of nuclear ribosomal DNA were amplified by two pairs of primers in 26 genotypes from the above species. Sequenced amplicons were aligned by ClustalW. Divergence matrices and phylogenic dendrogram were made by MEGA 5.0. Results revealed the full length of sequences of ITS1 and ITS2 were 650 bp and 700bp, respectively and G+C content were 60.25 and 60.50 in them. High levels of conservation in sequences were found among genotypes (%63 and %88). Phylogenetic analysis using amplified sequences were successfully divided diploid and polyploid wheats into individual groups. Regarding to the results, there were close relationships within T. aestivum and T. turgidum and also within T. urartu and T. boeticum. However, our analysis suggests that the ITS molecular markers seem to be proper tools for plant phylogenetic studies.


Main Subjects

  1. Baghaee-Ravari, S., Falahati-Rastegar, M., Jafarpour, B. & Shokoohifar, F. (2007). The study of probable variation in ITS- rDNA region of Fusarium solani in potato and its correlation with pathogenicity and geographical origin in Razavi and Northern Khorasan Provinces. Journal of Agricultural Sciences and Natural Resources, 14, 1-9. (in Farsi)
  2. Bandopadhyay, R., Sharma, Sh., Rustgi, S., Singh, R., Kumar, A., Singh-Balyan, H. & Kumar-Gupta, P. (2004). DNA polymorphism among 18 species of TriticumAegilops complex using wheat EST–SSRs. Plant Science, 166, 349-356
  3. Belgerami, S. (2009). The effects of ploidy and planting season on the phytic acid and some morphological characteristics of some species of grasses family. M.Sc. thesis. Faculty of Agriculture, University Shahrekord. (in Farsi)
  4. Calonje, M., Martın-Bravo, S., Dobes, C., Gong, W., Jordon-Thaden, I., Kiefer, C., Kiefer, M., Paule, J., Schmickl, R. & Koch, M. A. (2009). Non-coding nuclear DNA markers in phylogenetic reconstruction. Plant Systematics and Evolution, 282, 257-280.
  5. Carvalho, A., Guedes-Pinto, H. & Lima-Brito, J. (2009). Genetic variability assessed by ITS PCR- RFLP markers in old Portuguese bread wheat. Journal of Genetics, 88, 363-367.
  6. Doyle, J. J. & Doyle, J. L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemistry, 19, 11-15.
  7. Dvorák, J.  & Appels, R. (1982). Chromosomal and nucleotide sequence differentiation in genomes of polyploid Triticum species. Theoretical and Applied Genetics, 63, 349-360.
  8. Ehtemam, M H., Rahiminejad, M. R., Saeidi, H. & Ebrahim, F. (2014). Phylogenetic comparison of the A genome using karyotype analysis in some Triticum species. Taxonomy and Biosystematics, 21, 11-20.
  9. Golovnina, K. A., Glushkov, S. A., Blinov, A. G., Mayorov, V. I., Adkison, L. R. & Goncharov, N. P. (2007). Molecular phylogeny of the genus Triticum L. Plant Systematics and Evolution, 264, 195-216.
  10. Goryunova, S. V., Chikida, N. N., Gori, M. & Kochieva, E. Z. (2005). Analysis of Nucleotide Sequence Polymorphism of Internal Transcribed Spacers of Ribosomal Genes in Diploid Aegilops (L.) Species. Journal of Molecular Biology, 39, 173-176.
  11. Hammer, K., Filatenko, A.A. & Korzun, V. (2000). Microsatellite markers-a new tool for distinguishing diploid wheat species. Genetic Resources and Crop Evolution, 47, 497-505.
  12. Hsiao, C., Chatterton, N. J., Asay, K. H. & Jensen, K. B. (1995). Phylogenetic relationship of the monogenomic species of the wheat tribe, Triticeae (Poaceae), inferred from nuclear rDNA (internal transcribed spacer) sequences. Genome, 38, 211-223.
  13. Kharestani, H., A.A. Nasrolah-Nejad-Qomi. A. A. & Mehrabi, A. A. (2013). Genetic diversity assessment of Einkorn wheat by using microsatellite markers. Electronic Journal of Crop Production, 6, 1-16. (in Farsi)
  14. Mammdouh-Alnaddaf, L., Moualla, M. Y. & Haider, N. (2013). Genetic Relationships among Aegilops L. and Triticum L. Species based on the Internal Transcribed Spacer Sequences of nrDNA (ITS). Asian Journal of Agricultural Sciences, 5, 108-117.
  15. Mostafavi, G. (2013).Taxonomy and molecular phylogeny of land plants. Islamic Azad University, shahre rey. First Edition, 355 pp. (in Earsi)
  16. Nalini, E., Bhagwat, S. G. & Jawali, N. (2007). Identification and characterization of some ITS variants from hexaploid wheat (Triticum aestivum L.). Plant Science, 173, 262-268.
  17. Peacock, W. J., Gerlach, W. L. & Dennis, E. S. (1981) Molecular aspects of wheat evolution: repeated DNA sequences. In: Evans LT, Peacock WJ (eds) Wheat science today and tomorrow. Cambridge University Press, Cambridge, pp. 41-60.
  18. Wang, G. Z., Matsuoka, Y. & Tsunewaki, K. (2000). Evolutionary features of chondriome divergence in Triticum (wheat) and Aegilops shown by RLFP analysis of mitochondrial DNAs. Theoretical and Applied Genetics, 100, 221-231.
  19. Zhang, W., Qu, L. J., Gu, H., Gao, W., Liu, M., Chen, J. & Chen, Z. (2002). Studies on the origin and evolution of tetraploid wheats based on the internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA. Theoretical and Applied Genetics, 104, 1099-1106.
Volume 48, Issue 2 - Serial Number 2
September 2017
Pages 483-491
  • Receive Date: 11 May 2016
  • Revise Date: 07 August 2016
  • Accept Date: 09 August 2016
  • Publish Date: 23 August 2017