Genetic diversity of oily sunflower lines under normal and salt stress conditions using multivariate statistical analysis methods

Document Type : Research Paper


1 Ph.D. Student, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

2 Associate Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

3 Professor, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Urmia University, Urmia, Iran

4 Professor, Institute of Biotechnology, Urmia University, Urmia, Iran


To study genetic diversity of some agronomic traits and the effect of salt stress on these characters, 100 oily sunflower inbred lines coming from different regions of world was investigated under normal and salt (8 dS/m) stress conditions with randomized complete block design with three replications outside the greenhouse in an open air area. Analysis of variance showed significant differences among lines for all studied traits, indicating the existence of genetic variation among genotypes. The highest coefficient of genetic variation was observed for head dried weight, seed yield per plant and the lowest one for days to flowering in both stressed and non-stressed conditions. The results of correlation analysis revealed significant and positive correlation between seed yield per plant with most of the studied traits in both stressed conditions. Stepwise regression analysis revealed that under salt stress conditions, 40.3 percent of seed yield per plant variation was determined by head diameter, 100 seed weight, bottom leaf length, leaf number, bottom petiole length, upper leaf width and chlorophyll rate and in normal conditions, 30.3 of seed yield per plant variation was explained by head diameter, 100 seed weight and plant height. Cluster analysis grouped lines into 6 clusters in normal and 5 clusters in salt stress conditions but the distribution of lines within groups were different depending to stress environments that present the genetic variability for salt tolerance in sunflower lines. Lines 6, 11, 13 and 70 had the highest yield per plant under normal and salt stress conditions.


Main Subjects

  1. Akram, M., Ashraf, M. Y., Ahmad, R., Waraich, E. A., Iqbal, J. & Mohsan, M. (2010). Screening for salt tolerance in maize (Zea mays L.) hybrids at an early seedling stage. Pakistan Journal of Botany, 42, 141-154.
  2. Afzalifar, A., Zahravi, M. & Bihamta, M.R. (2011). Evaluation of tolerant genotypes to drought stress in Karaj region. Journal of Agronomy and Plant Breeding, 7, 25-44.
  3. Allakhverdiev, S. I., Sakamoto, A., Nishiyama, Y., Inaba, M. & Murata, N. (2000). Ionic and osmotic effects of NaCl-inactivation of photosystems I and II in Synechococcus spp. Journal of Plant Physiology, 123, 1047-1056.
  4. Ashraf, M., Athar, H. R., Harris, P. J. C. & Kwon, T. R. (2008). Some prospective strategies for improving crop salt tolerance. Advances in Agronomy, 97, 45-110.
  5. Ashraf, M. (2009). Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnology Advances, 27(1), 84-93.
  6. Asia Khaton, M., Qureshi, S. & Hssain, M. K. (2000). Effect of salinity on some yield parameters of sunflower. International Journal of Agriculture and Biology, 4, 382-384.
  7. Darvishzadeh, R., Pirzad, A., Hatami-Maleki, H., Poormohammad-Kiani, S. & Sarrafi, A. (2010). Evaluation of the reaction of sunflower inbred lines and their F1 hybrids to drought conditions using various stress tolerance indices. Spanish Journal of Agricultural Research, 8, 1037-1046
  8. Dehshiri, A., Modares Sanavi, M., Rezai, H. & Shirani Rad, A. (2012). Effect of elevated concentration of atomospheric carbon dioxide on some traits of three rapeseed (Brassica napus L.) varieties under saline conditions. Seed and Plant Production Journal, 28(2), 35-52. (in Farsi)
  9. Demir, M. & Ozturk, A. (2003). Effect of different soil salinity levels on germination and seedling growth of safflower (Carthamus tinctorius L.). Turkish Journal of Agriculture and Forestry, 27, 224-227.
  10. Dong, G. J., Liu, G. S. & Li, K. F. (2007). Studying genetic diversity in the core germplasm of confection sunflower (Helianthus annuus L.) in China based on AFLP and morphological analysis. Russian Journal of Genetics, 43, 627-635.
  11. Fareghi, S.H., Farshadfar, M. & Farshadfar, E. (2007). Study of chemical composition and nutrition value of prennial Lucerne (Medicago sativa L.) and genetic diversity based on SDS-PAGE markers. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 15(3), 196-210. (in Farsi).
  12. Farshadfar, A. (1997). The Application of Quantitative Genetics in Plant Breeding. Razi University Press, Kermanshah, Iran, Page 186.
  13. Farahbakhsh, M. & Towfighi, H. (1997). Relationship between electrical conductivity and total soluble salts content in salt affected soils of Iran. Iranian Journal of Agricultural Sciences, 28(2), 87-96. (in Farsi)
  14. Gepts, P. & Papa, R. (2003). Possible effects of (trans) gene flow from crops on the genetic diversity
    from landraces and wild relatives. Environmental Biosafety Research, 2, 89-103.
  15. Habib, H., Mehdi, S. S.  & Anjum, M. A. (2006). Genetic association and path analysis for seed yield in sunflower (Helianthus annuus L.). Pakistan Journal of Agricultural Sciences, 43(3-4), 136-139.
  16. Heidari, B. (2010). Genetic variation and genetic gain from selection in bread wheat. Electronic Journal of Crop Production, 3(3), 239-246. (in Farsi)
  17. Holland, J. B., Nyquist, W. & Cervantes, C. (2003). Estimating and interpreting heritability for plant breeding. Plant Breeding Reviews, 22, 9-112.
  18. Holland, J. B. (2006). Estimating genotypic correlations and their standard errors using multivariate restricted maximum likelihood estimation with SAS Proc MIXED. Crop Science, 46, 642-654.
  19. Hussain, S. A., Akhtar, J., Haq, M. A., Riaz, M. A. & Saqib, Z. A. (2008). Ionic concentration and growth response of Sunflower (Helianthus annuus L.) genotypes under saline and/or sodic water application. Soil & Environment, 27, 177-184.
  20. Ibrahim, O. M., Mohamed, M. H., Tawfik, M. M. & Badr, E. A. (2011). Genetic diversity assessment of barley (Hordeum vulgare L.) genotypes using cluster analysis. International Journal of Academic Research, 3, 81-85.
  21. Jayaraman, K. (1999). A Statistical Manual for Forestry Research. Food and Agricultural Organization of the United Nations Regional Office for Asia and the Pacific Bangkok, 234pp.
  22. Iqbal, M., Navabi, A., Salmon, D. F., Yang, R. C. & Spaner, D. (2007). Simultaneous selection for early maturity, increased grain yield and elevated grain protein content in spring wheat. Plant Breeding, 126, 244-250.
  23. Jafari, A. A., Seyed Mohammadi, R. & Abdi, N. (2007). Study of variation for seed yield and seed components in 31 genotypes of Agropyron desertorum through factor analysis. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 15(3), 211-221. (in Farsi)
  24. Khan, M. A., Shirazi, M. U., Khan, M. A., Mujtaba, S. M., Islam, E., Mumtaz, S., Shereen, A., Ansarian, R. U. & Ashraf, M.Y. (2009) . Role of proline, K+/Na+ ratio and chlorophyll content in salt tolerance of wheat. Pakistan Journal of Botany, 41(2), 633-638.
  25. Khan, M. A. & Gulzar, S. (2003). Germination responses of Sporobolus ioclados: A saline desert grass. Journal of Arid Environments, 55, 453-464.
  26. Khazaei, A. Moghaddam, M. & Noormohammadi, S. (2012).Genetic diversity of winter barley landraces collected from west of Iran. Iranian Journal of Crop Sciences, 13(4), 671-683. (In Farsi).
  27. Khodarahmpour, Z. (2011). Screening maize (Zea mays L.) hybrids for salt stress tolerance at germination stage. African Journal of Biotechnology, 10(71), 15959-15965.
  28. Kholghi, M., Darvishzadeh, R., Bernousi, I., Pirzad, A. & Laurentin, H. (2012). Assessment of genomic diversity among and within Iranian confectionery sunflower (Helianthus annuus L.) population by using simple sequence repeat markers. Acta Agriculturae Scandinavica Section B- Soil & Plant Science, 62(6), 488-498.
  29. Kumae, S. G., Reddy, A. M. & Sudhakar, C. (2003). NaCl effects on proline metabolism in two high yielding genotypes of mulberry (Morus alba L.) white contrasting salt tolerance. Plant Science, 165, 1245-1251.
  30. Mazinani, M. A., Moghaddam, M., Alavikia, S. S., Shakiba, M., Mehrabi, A. & Pouraboughaddareh, A. (2012). Study of genetic diversity in T. boeoticum populations under normal and water deficit stress conditions. Cereal Research, 2(1), 17-30. (in Farsi)
  31. Mostafavi, K. (2011). An evaluation of safflower genotypes (Carthamus tinctorius L.), seed germination and seedling characters in salt stress conditions. African Journal of Agriculture Research, 6(7), 1667-1672.
  32. Memon, S. A., Hou, X. & Wang, L. J. (2010). Morphological analysis of salt stress response of Pak Choi. Environmental, Agricultural and Food Chemistry,9, 248-254.
  33. Munns, R. (2002). Comparative physiology of salt and water stress. Plant Cell Environment, 25, 239-250.
  34. Munns, R. (2005). Genes and salt tolerance: bringing them together. New Phytologist, 167, 645-663.
  35. Naroui Rad, M., Alhadoo, M., Ghasemi, A. & Fanayi, H. (2009). Investigation of genetic diversity and broad sense heritability in watermelon accessions of Sistan. Iranian Journal of Horticultural Sciences, 40(4): 95-103. (in Farsi)
  36. Nezami, A., Khazaei, H. R., Boroumand Rezazadeh, Z. & Hosseini, A. (2008). Effects of drought stress and defoliation on sunflower (Helianthus annuus L.) in controlled conditions. Desert, 12, 99-104. (in Farsi)
  37. Noori, S. A., Ferdosizadeh, L., Izadi-Darbandi, A., Mortazavian, S. M. M. & Saghafi, S. (2011) Effects of salinity and laser radiation on proline accumulation in seeds of spring wheat. Journal of Plant Physiology and Breeding, 1(2), 11-20.
  38. Nooryazdan, H., Serieys, H., Bacilieri, R., David, J. & Berville, A. (2010). Structure of wild annual sunflower (Helianthus annuus L.) accessions based on agro-morphological traits. Genetic Resources and Crop Evolution, 57, 27-39.
  39. Parida, A. K. & Das, A. B. (2005). Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety, 60, 324-349.
  40. Poustini, K. & Siosemardeh, A. (2004). Ion distribution in wheat cultivars in response to salinity stress. Field Crops Research, 85, 125-133.
  41. Rui, L., Wei, S., Mu-Xiang, C., Cheng-Jun, J., Min, W. & Bo-Ping, Y. (2009). Leaf anatomical changes of Burguiera gymnorrhiza seedlings under salt stress.Journal of Tropical & Subtropical Botany,17, 169- 175.
  42. Shapiro, S. S. & Wilk, M. B. (1965). An analysis of variance test for normality. Biometrika, 52, 591-599.
  43. Soltani, A., Galeshi, S., Zenali, E. & Latifi, N. (2001). Germination, seed reserve utilization and growth of chickpea as affected by salinity and seed size. Seed Science and Technology, 30, 51-60.
  44. Shahbaz, M., Ashraf, M., Akram, N., Hanif, A., Hameed, S., Joham, S. & Rehman, R. (2011). Salt-induced modulation in growth, photosynthetic capacity, proline content and ion accumulation in sunflower (Helianthus annuus L.). Acta Physiologiae Plantarum,33, 1113-1122.
  45. Stansfield, W. D. (1991). Theory and Problems in Genetics. McGraw-Hill.
  46. Tahir, M. H. N., Imran, M. & Hussain, M. K. (2002). Evaluation of sunflower (Helianthus annuus L.) inbred lines for drought tolerance. International Journal of Agriculture and Biology, 3, 398-400.
  47. Yilmaz, H. & Kina, A. (2008). The influence of NaCl salinity on some vegetative and chemical changes of strawberries (Fragaria ananassa L.).African Journal of Biotechnology, 7, 3299- 3305.
  48. Zareh, M., Daneshiyan, J. & Zeynali Khaneghah, H. (2004). Genotypic variation in soybean cultivars for drought tolerance. Iranian Journal of Agriculture Science, 35, 859-886. (in Farsi)
  49. Zhao, G. Q., Ma, B. L. & Ren, C. Z. (2009). Salinity effects on yield and yield components of contrasting naked oat genotypes. Journal of Plant Nutrition, 32, 10-12.
  50. Zare Abyaneh, H., Jovzi, M., Afruzi, A. &  Gharibzadeh, A (2014) Determination of electrical conductivity of the saturation extract (ECe) relationships with a few soil salinity parameters in comparison with the new method of calculating ECe. Iranian of Irrigation & Water Engineering, 4(16), 83-94. (in Farsi)