Effects of drought stress on germination and photosynthetic pigments content of common bean (Phaseolus vulgaris L.) genotypes

Document Type : Research Paper

Authors

1 Former M. Sc. Student, Department of Agronomy and Plant Breeding, Agricultural Science and Engineering College, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professor, Department of Agronomy and Plant Breeding, Agricultural Science and Engineering College, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

Abstract

To evaluate the effects of drought stress on seed characteristics, seeds of the tolerant and sensitive genotyps of common bean were placed in petri dishes containing polyethylene glycol 6000 with concentration equal to -4, -8 and -12 bar, according to CRD Design with three replication using factorial arrengment, Nine day later, radicle and caulicle length were measured. The result showed that drought stress decreased germination speed and percentage, radicle and caulicle length, radicle fresh and dry weight, caulicle fresh and dry weight. To study the physiological processes, three genotypes including K-S-31167, GE-288 and Naz witch were respectivly tolerant, relatively tolerant and sensitive to drought stress were selected from first expriment and were subjected to 4 level of drought stress based on 25, 50, 75 and 100 percent of field capacity using CRD design with three replication using factorial arrengment, Droght stress was applied five weeks after planting and lasted for 10 days. Second terifoliate were used for sampling. The results indicated significant changes in the photosynthetic pigments content (PPC) and relative water content (RWC). Tolerant genotype (K-S-31167) had greater PPC and RWC compare to relatively tolerant genotype (GE-288) and sensitive genotype (Naz). This indicate that resistant genotype had higher membrane stability during stress.

Keywords

Main Subjects

References

  1. Arnon, D. T. (1949). Copper enzymes in isolation chloroplast phenoloxidase in (Beta vulgaris L.). Journal of Plant Physiology, 24, 1-15.
  2. Arshi, A., Ahmad, A., Aref, I. M. & Iqbal, M. (2012). Comparative studies on antioxidant enzyme action and ion accumulation in soybean cultivars under salinity stress. Journal of Environmental Biology, 33, 9-20.
  3. Barss, H. D. & Weatherley, P. E. (1962). A re examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Science, 15, 413-428.
  4. Bayoumi, T. Y., Manal, H. & Metwali, E. M. (2008). Application of physiological and biochemical indices as a screening technique for drought tolerance in bean genotypes. African Journal of Biotechnology, 14 (6), 2341-2352.
  5. Beebe, S. & McClafferty, B. (2006). ‘Bio-Fortified Beans’, Breeding Crops for Better Nutrition. HarvestPlus, CIAT, Cali-Colombia.
  6. Bhatt, R. M. & Srinivasa-Rao, N. K. (2005). Influence of pod load on response of okra to water stress. Indian Journal of Plant Physiology, 10, 54-59.
  7. Blume, A. & Ebercon, A. (1981). Cell membrane stability as a measure of drought and heat tolerance in wheat. Journal of Crop Scince, 27(1), 43-47.
  8. Boydak, M., Dirik, H., Tilki, F. & Calikoglu, M. (2003). Effects of water stress on germination in six provenances of Pinus brutia seeds from different bioclimatic zones in Turkey. Turkish Journal of Agriculture, 27, 91-97.
  9. Cazzonelli, C. I. (2011). Carotenoids in nature: insights from plants and beyond. Journal of Functional Plant Biology, 38, 833-847.
  10. De, F. & Kar, R. K. (1994). Seed germination and seedling growth of mung bean (Vigna radiate) under water stress induced by PEG-6000. Journal of Seed Science and Technology, 23, 301-304.
  11. Dodd, G. L. & Donovan, L. A. (1999). Water potential and ionic effects on germination and seedling growth of two cold desert shrubs. American Journal of Botany, 86, 1146-1153.
  12. Du, H., Wang, N., Cui, F., Li, X., Xiao, J. & Xiong, L. (2010). Characterisation of the beta-carotene hydroxylase gene DSM2 conferring drought and oxidative stress resistance by increasing xanthophylls and abscisic acid synthesis in rice. Journal of Plant Physiology, 154, 1304-1318.
  13. Ghaderi, N., Talaie, A. R., Ebadi, A. & Lessani, H. (2011). The physiological response of three Iranian grape cultivars to progressive drought stress. Journal of Agricultural Science and Technology, 13, 601-609.
  14. Ghanbari, A. A., Shakiba, M. R., Toorchi, M. & Choukan, R. (2013). Morpho-physiological responses of common bean leaf to water deficit stress. European Journal of Experimental Biology, 3(1), 487-492.
  15. Ghani, A., Azizi, M. & Tehranifar, A. (2009). Response of Achillea species to drought stress induce by polyethylene glycol in germination stage. Iranian Journal of Medicinal and Aromatic Plants, 25(2), 261-271. (in Farsi)
  16. Gunes, A., Inal, A., Adak, M. S., Bagci, E. G., Cicek, N. & Eraslan, F. (2008). Effect of drought stress implemented at pre-or post-anthesis stage on some physiological parameters as screening criteria in chickpea cultivars. Russian Journal of Plant Physiology, 55(1), 59-67.
  17. Heidari, N. & Purusef, M. (2011). Effect of seed priming with polyethylene glycol and sodium chloride on germination and growth indices of Pimpinella anisum L. Iranian Journal of Medicinal and Aromatic Plants, 27(3), 509-516. (in Farsi)
  18. Jaleel, C. A., Manivannan, P., Wahid, A., Farooq, M., Jasim, H., Somasundaram, R. & Pannerselvam, R. (2009). Drought stress in plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology, 11, 100-105.
  19. Kafi, M., Nezami, A., Hosaini, H. & Masomi, A. (2005). Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik.) genotypes. Iranian Journal of Field Crops Research, 3(1), 69-80. (in Farsi)
  20. Kaya, M. D., Okcu, G., Atak, M., Cıkılı, Y. & Kolsarıcı, O. (2006). Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24, 291-295.
  21. Khan, M. A. & Ungar, I. A. (1984). The effect of salinity and temperature on the germination of polymorphic seeds and growth of Atriplex triangularis willd. American Journal of Botany, 71(4), 481-489.
  22. Levitt, J. (1980). Responses of plants to environmental stress: Water, Radiation, Salt and Other Stresses. (2nd ed.). Academic Press, PP: NewYork, 3-211.
  23. Lawlor, D. W. & Cornic, G. (2002). Photosyntheticcarbon assimilation and associated metabolism in relation to water deficits in higher plants. Journal of Plant Cell and Environment, 25, 275-294.
  24. Macar, T. K., Turan, O. & Ekmekci, Y. (2009). Effects of water deficit induced by PEG and NaCl on chickpea (Cicer arietinum L.) cultivars and lines at early seedling stages. Gazi University Journal of Science, 22, 5-14.
  25. Maguire, J. D. (1962). Seed of germination aid in selection and evaluation for seedling emergence and vigour. Journal of Crop Science, 2, 176-177.
  26. Michel, E. B. & Kaufmann, M. K. (1972). The osmotic potential of polyeyhylene glycol 6000. Journal of Plant Physiology, 51, 914-916.
  27. Nikolaeva, M. K., Maevskaya, S. N., Shugaev, A. G. & Bukhov N G. (2010). Effect of Drought on Chlorophyll Content and Antioxidant Enzyme Activities in Leaves of Three Wheat Cultivars Varying in Productivity. Russian Journal of Plant Physiology, 57(1), 87-95.
  28. Parida, A. K., Das, A. B. & Mittra, B. (2004). Effects of salt on growth, ion accumulation, photosynthesis and leaf anatomy of the mangrove, Bruguiera parviflora. Journal of Trees, 18(2), 167-174.
  29. Rane, J., Maheshwari, M. & Nagarajan, S. (2001). Effect of pre-anthesis water stress on growth, photosynthesis and yield of six wheat cultivars differing in drought tolerance. Indian Journal of Plant Physiology, 6, 53-60.
  30. Ranjbarfordoei, A., Samson, R., Lemeur, R. & Van-Damme, P. (2002). Effects of osmotic drought stress induced by a combination of NaCl and polyethylene glycol on leaf water status, photo- synthetic gas exchange, and water use efficiency of Pistacia khinjuk and P. mutica. Journal of Photosynthetica, 40, 165-169.
  31. Rasool, S., Ahmad, A., Siddiqi, T. O. & Ahmad, P. (2013). Changes in growth, lipid peroxidation and some key antioxidant enzymes in chickpea genotypes under salt stress. Journal of Acta Physiologiae Plantarum, 35, 1039-1050.
  32. Saneoka, H., Moghaieb, R. E. A., Premachandra, G. S. & Fujita, K. (2004). Nitrogen nutrition and water stress effects on cell membrane stability and leaf water relations in Agrostis palustris Huds. Journal of Environmental and Experimental Botany, 52(3), 131-138.
  33. Sharifi, P., Amirnia, P., Majidi, R., Hashem, E., Roustaii, H., Nakhoda, M. B., Alipoor Mohammad, H. & Moradi, F. (2012). Relationship between drought stress and some antioxidant enzymes with cell membrane and chlorophyll stability in wheat lines. African Journal of Microbiology Research, 6(3), 617-623.
  34. Singh, S. P. (2007). Drought resistant in race Durango dry bean landraces and cultivars. Journal of Agronomy, 99, 1219-1225.
  35. Silva, M. D. A., Jifon, J. L., Da Silva, J. A. & Sharma, V. (2007). Use of physiological parameters as fast tools to screen for drought tolerance in sugarcane. Brazilian Journal of Plant Physiology, 19(3), 193-201.
  36. Soltani, E. (2014). Biochemical and molecular changes in a number of bean genotypes under drought stress. M. Sc. thesis. Faculty of Agricultural Science and Engineering, Department of Agronomy and Plant Breeding. University of Tehran, Karaj. (in Farsi)
  37. Zare, M., Mehrabi-oladi, A. A. & Sharafzadeh, Sh. (2006). Investigation of GA3 and Kinetin Effects on Seed Germination and Seedling Growth of Wheat under Salinity Stress. Journal of Agricultural Sciences, 12(4), 855-865.
  38. Zheng, Yh., Xu, Xb., Wang, My., Zheng, Xh., Li, Zj. & Jiang, Gm. (2009). Responses of salt-tolerant and intolerant wheat genotypes to sodium chloride: photosynthesis, antioxidants activities, and yield. Journal of Photosynthetica, 47, 87-94.

Files

History

  • Receive Date: 08 December 2015
  • Revise Date: 09 February 2016
  • Accept Date: 01 March 2016
  • Publish Date: 22 May 2017

Share

How to cite

Statistics