Effect of different storage conditions on germination and some biochemical characteristics of castor bean (Ricinus communis) seed

Document Type : Research Paper

Authors

1 M. Sc. Student, Department of Seed Science and Technology, Faculty of Agriculture, University of Yasouj, Iran

2 Assistant Professor, Department of Seed Science and Technology, Faculty of Agriculture, University of Yasouj, Iran

3 Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

4 Associate Professor, Department of Seed Science and Technology, Faculty of Agriculture, University of Yasouj, Iran

Abstract

In order to evaluate the effect of different storage conditions on seed germination and some biochemical characteristics of castor bean (Ricinus communis), a factorial experiments on the bases of completely randomized design with three factors included of storage temperature, seed moisture content (SMC) and storage duration in four replications was done in the seed laboratory of Faculty of Agriculture and Natural Resources, University of Tehran, Iran in 2014. Experimental factors were including five storage temperatures (5, 15, 25, 35 and 40oC), five SMC levels (3.53, 6, 9, 12 and 15%) and six storage duration (30, 60, 90, 120, 150 and 180 days). The results showed that in all storage temperatures germination percentage trend was descending with increasing storage temperature and duration. In all studied storage temperature, the seeds stored in SMCs of 3.53 and 15% showed the highest and lowest germination, respectively. However, the change in seed electrolyte leakage index was ascending by increasing the temperature and SMC. Also, with increasing storage duration, temperature and SMC, seed total protein content, enzymes activity of catalase and superoxide dismutase and ascorbate peroxidase decreased and malondialdehyde content (MDA) increased. Therefore, decrease in germination index and increase in electrical conductivity could be due to decline in antioxidant activities and increase in MDA content.

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References

  1. Aebi, H. (1984). Catalase in vitro. Methods Enzymol, 105, 121-126.
  2. Alivand, R. (2010). The study of deterioration in oil seed crops under different storage conditions. M.Sc Thesis. University of Tehran, Iran. 208 pp. (in Farsi)
  3. Ansari, O., Sharif-Zadeh, F., Moradi, A., Azadi, M.S. & Younesi, E. (2013). Heat shock treatment can improve some seed germination indexes and enzyme activity in primed seeds with gibberellin of Mountain Rye (Secale montanum) under accelerated aging conditions. Cercetari Agronomice în Moldova, 155(3), 21-30.
  4. Ansari, O. & Sharif Zadeh, F. (2012). Slow moisture content reduction (SMCR) can improve some seed germination indices in primed seeds of Mountain Rye (Secale montanum) under accelerated aging conditions. Journal of Seed Science and Technology, 2(2), 68-76.
  5. Bailly, C. (2004). Active oxygen species and antioxidants in seed biology. Seed Science Research, 14, 93-107.
  6. Bailly, C., Benamar, A., Corbineau, F. & Come, D. (2000). Antioxidant systems in sunflower (Helianthus annuusL.) seeds as affected by priming. Seed Science Research, 10, 35-­ 42.
  7. Bailly, C., Benamar, A., Corbineau, F. & Come, D. (1996). Changes in malondialdehyde content and superoxide dismutase, catalase, and glutathione reductase activities in sunflower seeds as related to deterioration during accelerated ageing. Physiologia Plantarum, 97, 104 -110.
  8. Basra, S. M. A., Ahmad, N., Khan, M. M., Iqbal, N. & Cheema, M. A. (2003). Assessment of cotton seed deterioration during accelerated aging. Seed Science and Technology, 31, 531-540.
  9. Benech-Arnold, R. L. & Sanchez, R. A. (2004). Handbook of seed physiology:  application to agriculture. Food Products Press, Binghamton, New York. 501 pp.
  10. Bradford, M. (1976). A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Analytical Biochemistry Analytical Biochemistry Analytical Biochemistry Analytical BiochemistryAnalytical Biochemistry, 72, 248-254.
  11. Chen, J., Cheng, Z. & Zhong, S. (2007). Effect of exogenous salicylic acid on growth and H2O2- metabolizing enzymes in rice seedlings under lead stress. Journal of Environmental Sciences, 19, 44-49.
  12. Chiu, K. Y., Wang, C. S. & Sung, J. M. (1995). Lipid peroxidation and peroxide-scavenging enzymes associated with accelerated aging and hydration of watermelon seeds differing in ploidy. Physiologia Plantarum, 94(3), 441 - 446.
  13. DemirKaya, M., Dietz, K. J. & Sivriteoe, H. O. (2010). Changes in antioxidant enzymes during ageing of onion Seed. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1), 49-52.
  14. Dhindsa, R. S. (1981). Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32(1), 93-101.
  15. Giannopolitis, C. N. & Ries, S. K. (1977). Superoxide dismutases I. Occurrence in higher plants. Plant physiology, 59(2), 309-314.
  16. Goel, A., Goel, A. K. & Sheoran, I. S. (2003). Changes in oxidative stress enzymes during artificial aging in Cotton (Gossypium hirstum L.) seed. Plant Physiology, 160, 1093-1100.
  17. Hampton, J. G. & TecKrony, D. M. (1995). Handbook of vigor test methods. The International Seed Testing Association, Zurich .117 pp.
  18. Heath, R. L. & Packer, I. (1968). Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125, 189-198.
  19. ISTA. (2010). International rules for seed testing. Published by the international seed testing Association.
  20. Kapoor, N., Arya, A., Siddiqui, M. A., Amir, A. & Kumar, H. (2010). Seed deterioration in chickpea (Cicer arietinum L.) under accelerated ageing. Asian Journal Plant Science, 9, 158-162.
  21. Kibinza, S., Vinel, D., Come, D., Bailly, C. & Corbineau, F. (2006). Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum, 128, 496-506.
  22. Kong, L., Huo, H. & Mao, P. (2015). Antioxidant response and related gene expression in aged oat seed. Frontier in Plant Science, 6, 1-9.
  23. Lin, R. H., Chen, K. Y., Chen, C. L., Chen, J. J. & Sung, J. M. (2005). Slow post-hydration drying improves initial quality but reduces longevity of primed bitter gourd seeds. Scientia Horticulturae, 106, 114-124.
  24. McDonald, M. B. (1999). Seed deterioration: Physiology, repair and assessment. Seed Science and Technolgy, 27, 177-237.
  25. Mehravar, M., Sateii, A., Hamidi, A., Ahmadi, M. R. & Salehi, M. (2014). Evaluation of lipid peroxidation and antioxidant enzyme activities in seeds of two soybean cultivars under the influence of accelerated aging. Iranian Journal of Seed Science and Technology, 3(1), 17-30. (in Farsi)
  26. Mohammadi, H., Soltani, A., Sadeghipour, H. R. & Zeinali, H. (2011). Effects of seed aging on subsequent seed reserve utilization and seedling growth in soybean. International Journal of Plant Production, 5(1), 65-70.
  27. Moradi, A. & Younesi, O. (2009). Effects of osmo-and hydro-priming on seed parameters of grain sorghum (Sorghum bicolor L.). Australian Journal of Basic and Applied Sciences, 3(3), 1696-1700.
  28. Mumtaz khan, M., Iqbal, J., Abbas, M. & Usman, M. (2003). Effect of aging on viability, vigour and chromosomal damage in pea (Pisum sativum L.) seeds. Pakistan Journal of Agricultural Sciences, 40(1), 54-50.
  29. Ogunniyi, D.S. (2006). Castor oil: A vital industrial raw material. Bioresource Technology, 97, 1086-1091.
  30. Ranieri, A., D’Urso, G., Nali, C., Lorenzini, G. & Soldatini G. F. (1996). Ozone stimulates apoplastic systems in pumkin leaves. Physiologia Plantarum, 97, 381-387.
  31. Rastegar, Z., Sedghi, M. & Khomari, S. (2011).  Effects of accelerated aging on soybean seed germination indices at laboratory conditions. Notulae Scientia Biologicae, 3(3), 126-129.
  32. Seiadat, S. A., Moosavi, A. & Sharafizadeh, M. (2012). Effect of seed priming on antioxidant activity and germination characteristics of maize seeds under different aging treatments. Research Journal of Seed Science, 5(2), 51-62.
  33. Seid Mohammadi, M. (2011). The effect of salicylic acid on temperature-moisture responses in aged seed of Brassica napus. M.Sc Thesis. University of Tehran, Iran. 378 pp. (in Farsi)
  34. Sofo, A., Dichio, B., Xiloyannis, C. & Masia, A. (2004). Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress. Physiologia Plantarum, 121, 58-65.
  35. Soltani, E., Galeshi, S., Kamkar, B. & Akramghaderi, F. (2008). Modeling seed aging effects on response of germination to temperature in wheat. Seed Science and Biotechnology, 2(1), 32-36.
  36. Stewart, R. R. C. & Bewley, J. D. (1980). Lipid peroxidation associated with accelerated aging of soybean. Plant Physiology, 65, 245-248.
  37. Zamani, A., Nouri, C., Tavakol Afshari, R., Iran Nejad, H., Akbari, GH. & Tavakoli, A. (2010). Evaluation of lipid peroxidation and activity of antioxidant enzymes in the seeds of safflower under conditions of natural and artificial aging. Iranian Journal of Agricultural Science, 41(3), 554-545. (in Farsi)

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History

  • Receive Date: 05 February 2016
  • Revise Date: 11 April 2016
  • Accept Date: 12 April 2016
  • Publish Date: 22 May 2017

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