Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

The use of viral translation enhancer and suppressor of gene silencing 2b to construct binary vector for high expression of protective antigen and lethal factor of the bacteria that cause anthrax

Document Type : Research Paper

Authors
Fariba Abooie Mehrizi 1
Alireza Abbasi 2
Hossein Honari 3
Houshang Alizadeh 4
1 Ph.D. Student, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
3 Associate Professor, Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
4 Assistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijfcs.2016.60132
Abstract
Bacillus anthracis as a spore-forming bacterium is the causative agent for anthrax. Three proteins from B. anthracis including protective antigen (PA), lethal factor (LF) and edema factor (EF) are causative factors of this bacteria. To produce plant-based vaccine, fusion protein of PA 4 domain and LF 1 domain via furin cleavage site joined to subunit B Vibrio cholera toxin (CTB) as an inducer of immune system. In this research we used 3’and modified 5’ untranslated regions of cowpea mosaic virus (CPMV) as translation enhancer and silencing inhibitor gene 2b of cucumber mosaic virus (CMV) to construct binary vector and transfer gene. Transient expression of tobacco and lettuce performed via agrobacterium and transient expression was confirmed at the level of transcriptome and proteome through RT-PCR and ELISA assay, respectively.
Keywords
CMV
CPMV
ELISA
transient expression
Subjects
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Iranian Journal of Field Crop Science
Volume 47, Issue 3 - Serial Number 3
Winter 2017
Pages 353-366

  • Receive Date 04 January 2016
  • Revise Date 11 January 2016
  • Accept Date 12 January 2016
  • Publish Date 21 November 2016