The use of viral translation enhancer and suppressor of gene silencing 2b to construct binary vector for high expression of protective antigen and lethal factor of the bacteria that cause anthrax

Document Type : Research Paper

Authors

1 Ph.D. Student, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

2 Associate Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

3 Associate Professor, Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran

4 Assistant Professor, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

Abstract

Bacillus anthracis as a spore-forming bacterium is the causative agent for anthrax. Three proteins from B. anthracis including protective antigen (PA), lethal factor (LF) and edema factor (EF) are causative factors of this bacteria. To produce plant-based vaccine, fusion protein of PA 4 domain and LF 1 domain via furin cleavage site joined to subunit B Vibrio cholera toxin (CTB) as an inducer of immune system. In this research we used 3’and modified 5’ untranslated regions of cowpea mosaic virus (CPMV) as translation enhancer and silencing inhibitor gene 2b of cucumber mosaic virus (CMV) to construct binary vector and transfer gene. Transient expression of tobacco and lettuce performed via agrobacterium and transient expression was confirmed at the level of transcriptome and proteome through RT-PCR and ELISA assay, respectively.

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References

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Iranian Journal of Field Crop Science
Volume 47, Issue 3 - Serial Number 3
January 2017
Pages 353-366

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History

  • Receive Date: 04 January 2016
  • Revise Date: 11 January 2016
  • Accept Date: 12 January 2016
  • Publish Date: 21 November 2016

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