Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Biochemical and molecular changes in three genotypes of beans (Phaseolus vulgaris L.) under drought stress

Document Type : Research Paper

Authors
Elham Soltani 1
Abdol Hadi Hosseinzadeh 2
Alireza Abbasi 2
1 Former M. Sc. Student, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
2 Associate Professor, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran
10.22059/ijfcs.2016.60120
Abstract
Drought stress is one of the major causes of reduction in crop production. Study physiological and biochemical processes related to drought stress can give better insight about mechanism of drought tolerance. To study the physiological and biochemical processes, three genotypes including K-S-31167, GE-288 and Naz were subjected to 3 level of drought stress based on 25, 50 and 100 percent of field capacity using CRD design with three replication using Factorial arrengment, Droght stress was applied five weeks after planting and lasted for 10 days. Second terifoliate were used for sampling. The results indicated significant changes in the electrolyte leakage (EL), prolin content (PC) and carbohydrates content (CC). Tolerant genotype (K-S-31167) had greater PC but lesser EL compare to relatively tolerant genotype (GE-288) and sensitive genotype (Naz). This indicates that tolerant genotype had higher membrane stability during stress. Results from measurement of soluble carbohydrate and expression of sucrose synthase and vacuolar invertase genes showed increase and decrease in the expression of these genes resulted in increase and decrease in the activity of the invertase and sucrose synthase enzyme which in turn influences the concentration of soluble materials which acts as metabolic signal in response to stress.
Keywords
common bean
Drought
expression gene
soluble carbohydrates
sucrose synthase
vacuolar invertase
Subjects
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Iranian Journal of Field Crop Science
Volume 47, Issue 3 - Serial Number 3
Winter 2017
Pages 401-411

  • Receive Date 05 December 2015
  • Revise Date 05 January 2016
  • Accept Date 23 January 2016
  • Publish Date 21 November 2016