ارزیابی اثر محلول‌پاشی اکسید روی به فرم معمول و نانوذرات بر فعالیت آنزیم‌های آنتی‌اکسیدانی و محتوای پرولین در دو رقم Zea Mays L. تحت تنش شوری

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری گروه زراعت و اصلاح نباتات، دانشگاه شاهرود، سمنان

2 استادیار گروه زراعت و اصلاح نباتات، دانشکدۀ کشاورزی دانشگاه صنعتی اصفهان

3 دانشجوی دکتری، دانشکدۀ کشاورزی، دانشگاه تبریز

چکیده

به‌منظور بررسی اثر محلول‌پاشی اکسید روی به دو فرم معمول و نانوذرات بر واکنش دو رقم ذرت به شوری، آزمایشی گلدانی در قالب طرح بلوک‌های کامل تصادفی در چهار تکرار اجرا شد. عوامل آزمایشی شامل دو رقم ذرت (تودۀ بذری و سینگل کراس 704)، سه تیمار تغذیۀ برگی (محلول‌پاشی اکسید روی به فرم معمول، به فرم نانوذرات و تیمار عدم محلول‌پاشی آب مقطر) و سه سطح شوری (صفر، 75 و 150 میلی‌مولار کلرید سدیم) بود. وزن خشک اندام هوایی در اثر شوری کاهش یافت و این کاهش در رقم سینگل کراس 704 بیشتر از تودۀ بذری بود. محتوای پرولین، مالون دآلدئید و فعالیت آنزیم‌های سوپراکسید دسموتاز، کاتالاز و آسکوربات پراکسیداز برگ در واکنش به تنش شوری افزایش یافت. میزان افزایش محتوای پرولین و فعالیت آنزیم‌های آنتی‌اکسیدانی در رقم تودۀ بذری در مقایسه با رقم سینگل کراس 704 بیشتر بود. محلول‌پاشی اکسید روی به دو فرم معمول و نانو سبب افزایش وزن خشک اندام هوایی و فعالیت آنزیم سوپراکسید دسموتاز هر دو رقم شد. صرف نظر از رقم، میزان افزایش فعالیت آنزیم آسکوربات پراکسیداز تحت تنش شوری در اثر محلول‌پاشی اکسید روی به فرم نانوذرات نسبت به فرم معمول آن کمتر بود. نتایج این آزمایش نشان داد که افزایش محتوای پرولین و فعالیت آنزیم‌های آنتی­اکسیدانی تودۀ بذری ذرت تحت تنش شوری بیشتر از سینگل کراس 704 بود. همچنین نتایج مطالعۀ حاضر بیانگر تأثیر مثبت و بارزتر فرم نانوذرات اکسید روی بر ارقام ذرت بود.

کلیدواژه‌ها

عنوان مقاله [English]

Effects of foliar application of ordinary and nano-particles of zinc oxide on the antioxidant enzyme activity and proline content of two Zea Mays L. cultivars under salt stress

نویسندگان [English]

  • Alireza Fathi 1
  • Morteza Zahedi 2
  • Shahram Torabian 3
1 Ph. D. Student, Sharood University, Semnan
2 Assistant Professor, Faculty of Agiculture, Isfahan University of Technology
3 Ph. D. Student, Faculty of Agriculture, Tabriz University
چکیده [English]

This experiment was conducted in order to evaluate the effects of foliar application of ordinary and nano-particles of zinc oxide on the response of corn to salinity. Treatments included two corn genotypes (seed mass and Sc 704), three spray levels (zinc oxide, nano-sized zinc oxide, and a spray of water treatment) and three salinity levels (0, 75 and 150 mM NaCl). Shoot dry matter was decreased as the level of salinity was increased and the reduction was greater in Seed mass than in Sc 704. However, the enzyme activity of superoxide dismutase (SOD), catalase (CAT) and ascorbat peroxidase (APX), and also the concentration of MDA and proline in leaves were increased under saline conditions. The extent of increases in proline content and the antioxidant enzyme activities was larger in Seed mass than in Sc 704. Foliar application of zinc oxide had significant positive effects on shoot dry matter and on the activity of SOD in leaves. Regardless of cultivars, the extent of increase in the activity of APX under saline condition was smaller when plants were sprayed with nano-sized zinc oxide compared to the ordinary bulk form. The results from this experiment showed that there were positive correlations between the increase in proline content and the enhanced antioxidant enzyme under saline condition and the salt tolerance of the more salt tolerant cultivar. Although, there were no significant differences between the two tested cultivars in response to zinc oxide foliar application, positive effects of nano-sized was more than ordinary form.

کلیدواژه‌ها [English]

  • Nano-sized
  • Catalase
  • peroxides
  • superoxide dismutase
  • Single Cross704
  • Foliar Application

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سابقه مقاله

  • تاریخ دریافت: 17 آذر 1393
  • تاریخ بازنگری: 13 فروردین 1394
  • تاریخ پذیرش: 13 فروردین 1394
  • تاریخ انتشار: 01 تیر 1394

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