Genetic Diversity of Aegilops triuncialis L. Accessions of Iran Revealed by microsatellite markers

Document Type : Research Paper

Authors

1 Associate Professor, Plant Breeding, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Ilam

2 Former Graduate Student Plant Breeding, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Ilam

3 Former Graduate Student, Biotechnology, Faculty of Agriculture and Natural Resources, Islamic Azad

4 Professor, Biotechnology, Faculty of Agriculture and Natural Resources, Islamic Azad University; Branch of Science and Research (Tehran)

Abstract

 
In order to study the genetic diversity among 35 accessions of Ae. triuncialis L. from17 SSR loci of 56 primer pairs originated from A and D genomes of bread wheat were used. Seventy one fragments amplified totally and 68 of them were polymorph between genotypes. Allele number ranged from 1 to 8 with average 4.18 per locus. PIC value of markers was from 0.593 (Xgwm-30-2D, Xgwm-383-3D, Xgwm-654-5D) to 0.861(Xgwm-156-5A) and marker index varied from 1.779 to 6.888. Average genetic distance of genotypes was 0.859. Two accessions from Lorestan and Khoozestan with least genetic distance (0.231) was the most similar genotypes. Cluster analysis was not able to separate accessions from each other.  No relationships between genetic and geographic variation indicates high amount of genetic diversity among genotypes. All molecular variation was from within groups which indicate no specific SSR alleles for accessions. This genetic diversity and transferability of known SSRs in cultivated could be assigned as useful tools to management of wild wheat genetic resources.

Keywords

References

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History

  • Receive Date: 19 August 2014
  • Revise Date: 10 March 2015
  • Accept Date: 07 April 2015
  • Publish Date: 22 June 2015

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