Putrescine induces drought tolerance and alters the activities of antioxidant enzymes in growing chamomile plants (Matricaria Chamomilla L.)

Document Type : Research Paper


1 Ph. D. Student, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

2 Professor, Department of Agronomy and Plant Breeding, University College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

3 Assistant Professor, Medicinal Plants and Drugs Research Institute, University College of Agriculture, University of Shahid Beheshti, Tehran, Iran


The present study focused on the influence of Put treatment on antioxidant enzyme activity in chamomile seedlings under drought stress. Treatments included two moisture regimes (87and 43درصد of field capacity), putrescine application (0 and 0.1 mM), and two chamomile cultivars (Bodgold and Hungary breed seed). The experiment was conducted in a factorial design based on randomized complete blocks with three replicates. Drought reduced soluble protein content, and increased the activities of antioxidant enzymes, including ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (GPX) glutathione reductase (GR). Put treatment caused a significant increase in the activity of antioxidant enzymes, as compared to control (respectively 26.6, 17, 26.56 and 8.88درصد for APX, CAT, GPX and GR), while no significant effect was recognized so long as the soluble proteins content was taken into account. A significant association was observed between moisture regimes and Put in terms of GR activity (P < 0.05), while interaction between cultivar and moisture regimes was only significant as the variable of GPX was considered (P < 0.05). Interestingly, between two cultivars, Bodgold was found to contain higher GR and GPX activities compared to Hungary breed seed. While Hungary breed seed exhibited higher soluble proteins content, APX and CAT activities than the other one.


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