Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Proteome analysis of wheat seed embryo (Tritium aestivum) in tolerant and susceptible cultivars under drought stress during early germination phase

Document Type : Research Paper

Authors
Ali Shayanfar 1
Reaza Tavakkol Afshari 2
Houshang Alizade 3
Abolrahman Rasoulnia 1
1 Former Graduate Student, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj
2 Professor, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj
3 Assistant Professor, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj
10.22059/ijfcs.2015.54868
Abstract
Germination is one the most important growth phases in plants that injury from drought stress extremely. Germination divided to 3 phases although before third phases, many activity (radicle protrusion) initiated. Many metabolically activity occurred specially in first phase since seed embryo is a live organ, although differences in protein patterns arised in this phase. This study was carried out to understand the effect of drought stress on protein patterns in the first phase of germination. In order to analysis the effects of drought stress (-12 bar) on wheat embryo proteome (Sardari and Qods respectively tolerant and susceptiblecultivar), factorial experiment was conducted in a Randomized completely block design with three replications. Drought stress diminished germination percentage and caused adverse effects on the proteins pattern. Two-dimensional gel electrophoresis analysis of wheat embryo axis showed 1000 spots were reproducible that 25 spots between treatments indicated significant differences on 5درصد level. In control condition, in comparison tolerant to susceptiblecultivar, 22 and 6 spots and in stress condition, 21 and 10 spots were down and up regulated respectively. The results indicated that frequency of protein under drought stress depends on severity and amount of stress and imbibition time.
 
Keywords
embryo seed
germination
Proteomics
Wheat
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Iranian Journal of Field Crop Science
Volume 46, Issue 2 - Serial Number 2
Summer 2015
Pages 207-215

  • Receive Date 14 September 2014
  • Revise Date 24 February 2015
  • Accept Date 03 May 2015
  • Publish Date 22 June 2015