Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Effect of Cryopreservation by Vitrification on the Growth and Development Indices of Rice Zygotic Embryos

Document Type : Research Paper

Authors
Seyede Zahra Tabatabaiepoor 1
Ahmad Moieni 2
Mohammad Sadegh Sabet 3
1 M. Sc. Student of Plant Breeding, Faculty of Agriculutre, Tarbiat Modares University, Tehran
2 Associate Professor, Faculty of Agriculutre, Tarbiat Modares University, Tehran
3 Assistant Professor, Faculty of Agriculutre, Tarbiat Modares University, Tehran
10.22059/ijfcs.2015.54051
Abstract
Because of the importance of Iranian rice germplasm and its application in the rice breeding programs and international genetic resources exchanges, some researches are necessary to preserve these valuable genetic resources. For this purpose, the cryopreservation of the biological samples in liquid nitrogen at -196 °C can be used. In this research, the cryopreservation of the zygotic embryos of seven rice indigenous and modern cultivars was investigated by vitrification method. The embryos were stored in the liquid nitrogen for 1, 7 and 30 days. After cryopreservation, the embryos were transferred at 38-40 °C for 2 min and then were cultured on MS medium. There were no significant differences among the studied cultivars in the final germination percentage trait, but there were significant differences in vigor index trait in level %5 and in other studied traits in level %. In most traits, there was no significant difference among the storage times in liquid nitrogen while there was a significant decrease in the studied traits in comparison with the control. Investigation of the other cryopreservation methods and treatments on Iranian rice indigenous cultivars germplasm could be recommended
Keywords
Rice germplasm
Cryopreservation
Zygotic embryo
  1. Amiri Ardakani, M. (2007). Indigenous Knowledge in Rice Farming. Esfahan: Sabouh Publication. (In Farsi)
  2. Aryakia, E., Ramazani, H., Ghafoori, H., Dolatyari, A., Naghavi M. R. & Shahzadeh fazeli S. A. (2012). The effect of cryopreservation on germination and growth indices of some orthodox seeds. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 19, 218-230. (In Farsi)
  3. Arzani, A. (2004). Breeding Field Crops (Authored by: Sleper & Poehlman). 3th edition. Isfahan University of Technology Publication. (In Farsi)
  4. Benson, E. E.(2008). Cryopreservation of phytodiversity: a critical appraisal of theory and practice. Critical Reviews in Plant Sciences, 27, 141-219.
  5. Beardmore, T. & Whittle, C. A. (2005). Induction of tolerance to desiccation and cryopreservation in silver maple (Acer saccharinum) embryonic axes. Tree Physiology,25, 965-972.
  6. Brunakova, K., Zamecnik, J., Urbanova, M. & Cellarova, E.(2011). Dehydration status of ABA-treated and cold-acclimated Hypericum perforatum L. shoot tips subjected to cryopreservation. Thermochimica Acta, 525, 62-70.
  7. Chmielarz, P. (2009). Cryopreservation of dormant European ash (Fraxinus excelsior) orthodox seeds. Tree Physiology, 29, 1279-1285.
  8. Gale, S., John, A., Harding, K. & Benson, E.(2008). Developing cryopreservation for Picea sitchensis (sitka spruce) somatic embryos: a comparison of vitrification protocols. CryoLetters, 29, 135-144.
  9. Gonzalez-Arnao, M. T. & Engelmann, F. (2006). Cryopreservation of plant germplasm using encapsulation-dehydration technique: Review and case study on sugarcane. CryoLetters, 27, 155-168.
  10. Harding, K. (1996). Approaches to assess the genetic stability of plants recovered from in vitro culture. In: Normah, M. N., Narimah, M. K. & Clyde, M. M. (eds). In-vitro conservation of plant genetic resources. University Kebangsaan, Malaysia, pp 135-168.
  11. Harding, K. & Benson, E. E. (1994). A study of growth, flowering and tuberisation in plants derived from cryopreserved potato shoot-tips: implications for in vitro germplasm collections. CryoLetters, 15, 59-66.
  12. Hatami1, F., Jebelli, M., Naderi Shahab, M., Tabari, M. & Jafari, A. A. (2010). Cryopreservation of Acer monspessulanum seeds. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 18(1), 12-23.
  13. Ishikawa, K., Harata, K., Mii, M., Sakai, A., Yoshimatsu, K. & Shimomura, K.(1997). Cryopreservation of zygotic embryos of a Japanese terrestrial orchid (Bletilla striata) by vitrification. Plant Cell Reports,16, 754-757.
  14. Jafari-Mofidabadi, A. (2007). Tissue Culture Methods in Breeding and Reproduction of Plants. Anemone Village, Publication. (In Farsi)
  15. Kami, D., Shi, L., Sato, T., Suzuki, T. & Oosawa, K. (2009). Cryopreservation of shoot apices of hawthorn in vitro cultures originating from East Asia. Scientia Horticulturae, 120, 84-88.
  16. Kandil, A. A., Sharief A. E. & Nassar, E. S. E.(2012). Response of some rice (Oryza sativa L.) cultivars to germination under salinity stress. International Journal of Agriculture Sciences, 4, 272-277.
  17. Kaviani, B., Padasht Dehkaei, M. N., Hashemabadai, D. & Darabi, A. H. (2010). Cryopreservation of Lilium Ledebourii (Baker) Bioss. By encapsulation-vitrification and in vivo media for planting of germplasm. American Eurasian Journal of Agricultural and Environmental Sciences, 8(5), 556-560.
  18. Khodabandeh, N. (2000). Cereals (6th ed). Tehran University Publications. (In Farsi)
  19. Matsumoto, T., Mochida, K., Itamura, H. & Sakai, A.(2001). Cryopreservation of persimmon (Diospyros kaki Thunb.) by vitrifiation of dormant shoot tips. Plant Cell Report, 20,398-402.
  20. Matsumoto, T., Sakai, A. & Yamada, K. (1994). Cryopreservation of in vitro grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration. Plant Cell Reports, 13, 442-446.
  21. Murashige, T. & Skoog, F.(1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum,15,473-497.
  22. Panis, B., Piette, B. & Swennen, R. (2005). Droplet vitrification of apical meristems: a cryopreservation protocol applicable to all Musaceae. Plant Science, 168, 45-55.
  23. Popov, A. S., Popova, E. V., Nikishina, T. V. & Vysotskaya, O. N. (2006). Cryobank of plant genetic resources in Russian Academy of Sciences. International Journal of Refrigeration, 29, 403-410.
  24. Raghavan, V. (2003). One hundred years of zygotic embryo culture investigations. In Vitro Cellular and Developmental Biology – Plant, 39, 437-442.
  25. Ravathi, S. & Pillia, M. A. (2011). In vitro callus induction in rice (Oryza sativa L.). Research in Plant Biology, 1(5), 13-15.
  26. Reed, B. M. (2008). Plant Cryopreservation: A Practical Guide. New York: Springer.
  27. Rong, H. S. & Hua, Y. M. (2009). High-efficiency vitrification protocols for cryopreservation of in vitro grown shoot tips of rare and endangered plant Emmenopterys henryi Oliv. Plant Cell, Tissue and Organ Culture, 99, 217-226.
  28. Sakai, A., Kobayashi, S. & Oiyama, I. (1990). Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Reports. 9, 30-33.
  29. Sershen, Varghese, B., Pammenter, N. W. and Berjak, P. (2012). Cryo-tolerance of zygotic embryos from recalcitrant seeds in relation to oxidative stress-A case study on two amaryllid species. Journal of Plant Physiology, 169, 999-1011. Available on the www.SciVerseScienceDirect.com.
  30. Shatnawi, M. A.(2011). Cryopreservation of Capparis spinosa shoot tips via vitrification, encapsulation dehydration and encapsulation vitrification. World Applied Sciences Journal, 15, 318-325.
  31. Suzuki, M., Ishikawa, M. & Akihama, T.(2005). Cryopreservation of encapsulated gentian axillary needed to increase recovery of tips after cryopreservation buds following 2 step preculture with sucrose and desiccation. Plant Cell, Tissue and Organ Culture, 83, 115-121.
  32. Tajadod, G., Farzami sepehr, M. & Kalami, Z. (2010). The effect of two plant growth regulators on callus generation of Oryza Sativa L. var Hashemi. Journal of Biology iau-garmsar, 5(3), 13-20. (In Farsi)
  33. Thorpe, T. A. and Yeung, E. C. (2011). Plant Embryo Culture: Methods and Protocols, Humana Press, Springer Science, Business Media, Spring Street, New York, USA.
  34. Usman, I. S. & Abdulmalik, M. M.(2010). Cryopreservation of embryonic axes of maize (Zea mays L.) by vitrification protocol. African Journal of Biotechnology,9,8955-8957.
  35. Wang, Y. L., Fan, M. J. & Liaw, S. I. (2005). Cryopreservation of in vitro-grown shoot tips of papaya (Carica papaya L.) by vitrification. Botanical Bulletin of Academia Sinica, 46, 29-34.
  36. Wen, B., Wang, R., Cheng, H. & Song, S. (2010). Cytological and physiological changes in orthodox maize
  37. embryos during cryopreservation. Protoplasma, 239, 57-67.
  38. Withers, L. A. (1982). The development of cryopreservation techniques for plant cell, tissue and organ culture. In: Fujiwara A (ed), In: Proceedings of 5th International Congress Plant Cell Tissue Culture, Tokyo, Japan, pp 793–794.
Iranian Journal of Field Crop Science
Volume 46, Issue 1 - Serial Number 1
Spring 2015
Pages 115-122

  • Receive Date 25 May 2014
  • Revise Date 04 December 2014
  • Accept Date 16 December 2014
  • Publish Date 21 March 2015