Study of genetic diversity and clustering of barley (Hordeum vulgare L.) genotypes to resistance of powdery mildew disease in seedling stage

Document Type : Research Paper

Authors

1 MSc student of Plant Breeding, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

2 Assistant professor of Department of Agronomy & Plant Breeding, University of Guilan, Rasht, Iran.

3 Assistant professor of Department of Plant Protection respectively, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran

Abstract

ABSTRACT
This study was conducted to evaluate the resistance of 70 genotypes of barley to powdery mildew. After preparing the samples contaminated with powdery mildew, reproduction of the spores of the fungus was conducted on susceptible cultivar Afzal. The experiment was conducted with randomized block design in pot in greenhouse. Seedlings were inoculated with the spores fungus in two-leaf stage. After 12 days, the traits of the infection type and infection intensity, assessed based on 0-9 Saari and Prescott scale. After comparing the means, to obtain an overall result, ranking of genotypes were conducted based on the comparison of means with Arunachalam method. The results showed that varieties Afzal, Rihane, Goharjow, EB-88-8, 45-Motadel, EC-83-17 and Fajre 30 received the highest ranking for infection type and disease intensity and were identified as susceptible and moderately susceptible genotypes. The genotypes EB-86-6, EB 87-20, Arass, EB-88-2, EBYT-W-89-4, EB-88-13, Dasht, MB-83-14, EBYT-W-89-3, NB17, 24 Garm, EB-89-19, EBYT-W-89-15 and W-79-10 allocated lowest ranking and found as resistance genotypes. The results of cluster analysis were endorsing the results of ranked Arunachalam. According to the analysis, genotypes were divided into four groups, resistant, moderately resistant, moderately susceptible and susceptible. Considerable estimate for phenotypic and genotypic coefficient of variation and broad heritability for infection type and infection intensity indicates selection likely can be effective in improvement of resistance to powdery mildew.
 

Keywords

References

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History

  • Receive Date: 27 July 2014
  • Revise Date: 02 November 2014
  • Accept Date: 06 December 2014
  • Publish Date: 21 March 2015

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