Genetic diversity of rapeseed (Brassica napus L.) using SSR and ISJ molecular markers

Document Type : Research Paper

Authors

1 Former MSc Student, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol.

2 Associate Professor of Plant Breeding, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol.

3 Assistant Professor of Plant Breeding, Department of Agronomy, Faculty of Agriculture, University of Lorestan.

4 Associate Professor of Plant Disease, Department of Plant Protection, Faculty of Agriculture, University of Zabol

Abstract

To investigate the genetic diversity of 21 rapeseeds (Brassica napus L.), 20 semi-random ISJ and SSR primers were used. Primers produced a total number of 158 (81.5%) polymorphic bands (51 SSR and 107 ISJ bands). Average numbers of polymorphic bands for ISJ, SSR and combined of them were 10.7, 5.1 and 7.9, respectively. PIC values ranged from 0.0150 for specific primer O110D08 to 0.3420 for semi-random primer ET15-33. No significant differences were found between IT and ET primers with regard to polymorphic bands. According to UPGMA cluster analysis and Dice similarity matrix, rapeseed cultivars were divided in 3, 6 and 4 groups for ISJ, SSR primers and combine of them, respectively. Genetic variations don’t agreed with geographic pattern and Iranian and foreign cultivars were not grouped in separate clusters, indicating presumably the proximity and kinship of Iranian and foreign genotypes. SLM096 and Licord with same origin produced separate groups and had high genetic distance with other genotypes; therefore, they can be used in the future breeding programs.

Keywords

References

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History

  • Receive Date: 12 October 2013
  • Revise Date: 11 December 2014
  • Accept Date: 18 December 2014
  • Publish Date: 21 March 2015

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