Effect of salinity stress on germination parameters, chlorophyll content and antioxidant enzyme activity in chickpea (Cicer ariantaum L.) genotype

Document Type : Research Paper


1 Association professor of Department of Agronomy & Plant Breeding Faculty of Agricultural Science & Engineering College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran

2 Ph.D of student seed science and technology Department of Agronomy Faculty of Plant Production Gorgan University of Agricultural Science and Natural Resources (GUASNR) Gorgon, Iran

3 Ph.D of student Department of Agronomy & Plant Breeding Faculty of Agricultural Science & Engineering College of Agriculture & Natural Resources, University of Tehran, Karaj, Iran


To study the effect of salinity on physiological and morphological traits of chickpea in 2010 at Faculty of Agriculture and Natural Resources, University of Tehran With Seven chickpea genotypes. Two experiments design was RCBD with 3 replications and 5 levels of salinity stress (Ctrl, 200, 150, 100, 50 mM) for laboratory experiment on germination indices and greenhouse experiment on physiological and biochemical traits. Some traits were recorded such as germination percentage, root length, shoot length and vigor index in laboratory. According to the results, mentioned traits had linear response with increasing of salinity stress. Highest and lowest of germination percentage were obtained in 200 mM treatment for G485 and G456 respectively. G485 had highest vigor index in response to 200 mM treatment. The pea genotypes of biomass was significantly affected by salinity levels, , so the stress level of 50 mM G485 with 3.8 g had the highest levels of the index.In second study, we evaluate activities of catalase, peroxidase, polyphenol oxidase, total chlorophyll and carotenoids and biomass. G104 genotype had highest of Catalase activity (1.75unit) and highest of Proxidase enzyme activity and polyphenol oxidase were obtained by G485 genotype in 200mM treatment. Also highest (93%) and lowest (54%) reduction percentage of chlorophyll content were observed in G643 and G485 respectively. Results showed a high correlation between antioxidant enzyme activities and chlorophyll, so that Genotypes with higher antioxidant activity had higher chlorophyll content and biomass. There was positive correlation between resistant to salinity at seedling stage of germination and greenhouse condition.


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