Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

QTL mapping of genes controlling Na+ and K+ concentration in roots and shoots of wheat under normal and salt stress conditions

Document Type : Research Paper

Authors
Bahram Masoudi 1
Eslam Majidi Hervan 2
Mohsen Mardi 3
Mohammad-Reza Bihamta 4
Mohammad-Reza Naghavi 4
Babak Nakhoda 5
Ashkboos Amini 6
1 Ph.D. Student, Department of plant breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Prof., Department of Molecular Physiology, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
3 Associate Prof., Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
4 Professors, Department of plant breeding, The University of Tehran, Karaj, Iran.
5 Prof., and Assistant Prof., Department of Molecular Physiology, Agricultural
6 Instructor, Seed and Plant Research Institute, Karaj, Iran
10.22059/ijfcs.2014.53531
Abstract
In wheat Na+ exclusion and K+/Na+ have shown to be associated with salinity tolerance, therefore in order to identify QTLs with additive effect for Na+ and K+ concentration traits in roots and shoots of wheat, 319  bread wheat recombinant inbred lines (RIL F7), derived from a cross between Roshan cultivar (salt tolerant) and Falat cultivar (salt sensitive), along with their parents and 2 checks (Arg and Moghan3) were studied in 2 separate randomized complete block designs (normal and stress) with 3 replications in greenhouse in 2012. Traits measured included shoot and root Na+ and K+ concentration, K+/Na+ ratio in root and shoot and root to shoot Na+ and K+ concentrations. Linkage map was constructed with 730 markers (709 DArt markers and 21 SSR markers). The linkage map spanned 4505.71 cM with an average distance of 6.17 cM between adjacent markers. A total of 31 additive QTLs were identified by QTL Cartographer program using single-environment phenotypic values. Results indicated that the biochemical pathways for Na+ and K+ accumulation are highly likely to be independent. Also, results indicated that shoot Na+ concentration and root to shoot Na+ and K+ concentrations could be used as selection criteria between tolerant and sensitive cultivars in salt stress condition.
 
Keywords
Physiological traits
QTL
salt stress
bread wheat
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Iranian Journal of Field Crop Science
Volume 45, Issue 3 - Serial Number 3
Autumn 2014
Pages 345-353

  • Receive Date 16 April 2013
  • Revise Date 19 May 2015
  • Accept Date 12 October 2013
  • Publish Date 23 September 2014