Genetic diversity among accessions of Aegilops triunsialis L. from west of Iran using ISSR molecular markers

Fathi, Tahereh; Souhani, Mohammad Mahdi; Samizadeh Lahiji, Habibollah; Mehrabi, Ali Ashraf

doi:10.22059/ijfcs.2014.51030

Genetic diversity among accessions of Aegilops triunsialis L. from west of Iran using ISSR molecular markers

Document Type : Research Paper

Authors

Tahereh Fathi ¹

Mohammad Mahdi Souhani ²

Habibollah Samizadeh Lahiji ³

Ali Ashraf Mehrabi ⁴

¹ MSc. Student, Faculty of Agricultural Sciences, University of Guilan, Iran

² Assistant Professor, Faculty of Agricultural Sciences, University of Guilan, Iran

³ Associate Professor, Faculty of Agricultural Sciences, University of Guilan, Iran

⁴ Assistant Professor, Faculty of Agricultural Sciences, University of Ilam, Iran

10.22059/ijfcs.2014.51030

Abstract

West part of Iran is considered as one of the center of diversity for Aegilops triunsialis, therefore, study of its genetic diversity in this area is extremely important regarding evolution of this plant. The genetic variation among 40 accessions of Ae.triunsialis collected from west of Iran were studied using 12 ISSR markers. DNA was extracted, concentrations adjusted and five samples from each accession were bulked. Totally, 118 polymorphic bands were produced using 12 primers that UBC817 primer with 16 and then UBC811 and UBC823 with 14 bands had the maximum number, while UBC825 with 4 bands had minimum polymorphic bands. The PIC value varied from 0.23 to 0.40 and the MI value between 1.1 and 4.6. Principal Coordinates Analysis showed that the first two could explain the total variation of 23.72 percent. Cluster analysis using Neighbor Joining, placed the 40 accessions in three clusters that each contain 13, 9, and 18 members, respectively. Discriminate Function via Fisher's linear confirmed the validity of clustering analysis result (95%).

Keywords

Analysis of molecular variance

discriminant function analysis

marker index

Polymorphism Information Content