Developement of sugar beet lines resistant to rhizomania using doubled haploid technique

Using haploid technique in breeding of sugar beet as an allogamous plant will facilitate breeding efforts. In this method,a pure line with a desirable genotype can be achieved by unfertilized ovule culture. Therefore ovule culture was done from populations with numbers: 27621, 27624, 27626 and 27629. Young flowering stems were collected and ovules were isolated from the flower buds after sterilization and cultured in N6 medium containing 0.2 mg/l BA,0.5 mg/l NAA,6% sucrose ,0.1% activated charcoal and 0.8% agar and kept at 27ºC in dark condition for one month. Ovules with direct regeneration transferred to medium containing 0.5 mg/l BA, 0.2 mg/l NAA and 3% sucrose after four weeks. Calli were transferred to the regeneration medium. The percentage of cultured ovules response was between 1.6-4%. Then haploids micro shoots were cultured in basic medium containing 0.1% colchicines and 1.5% DMSO for 24 hours, and were subsequently transferred to basic medium with a low level hormone. Haploid micro shoots were cultured in basic medium containing 0.1% colchicines and 1.5% DMSO for 24 hours, and were subsequently transferred to basic medium with a low level hormone .Rooting of DH plants was done in vitro.