Construction of pBI121: EXPB2 and its Transformation into Arabidopsis thaliana

Document Type : Research Paper

Authors

1 Graduate student College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

2 Assistant Professor College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

3 Professor College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

4 Graduate Student College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Abstract

Plant growth is a result of cell division and cell enlargement. Expansins are proteins that mediate long-term extension of isolated cell walls and they play a variety of roles in plant cells. In this study the AtEXPB2 gene was over-expressed in Arabidopsis thaliana. The gene was isolated from genomics DNA of Arabidopsis and cloned into mediated vectors; pGEMT and pBI121 vectors. Transformation of Arabidopsis thaliana was performed by floral dip method using Agrobacterium-mediated.  The produced seeds by infected plants were planted on MS medium containing kanamycin antibiotics, then, the tolerant seedlings were transferred to pot. Polymerase chain reaction was used to confirm the transgenic plants. RT-PCR analysis with AtEXPB2 specific primers showed that transferred gene is active and expressed. Obtained results illustrated that over-expression of expansin family genes may utilize for developing drought tolerant plants.

Keywords

Iranian Journal of Field Crop Science
Volume 44, Issue 2 - Serial Number 2
September 2013
Pages 191-197

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History

  • Receive Date: 02 September 2012
  • Revise Date: 08 September 2013
  • Accept Date: 19 February 2013
  • Publish Date: 23 August 2013

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