Co-Regulation and Co-Expression Study of Some Salinity-Response Genes in Bread Wheat (Triticum Aestivum L.)

Abouyee Mehrizi, Fariba; Abbassi, Alireza; Shah Nejat Boushehri, Ali Akbar; Yazdi Samadi, Bahman; Alizade, Hooshang

doi:10.22059/ijfcs.2012.29048

Co-Regulation and Co-Expression Study of Some Salinity-Response Genes in Bread Wheat (Triticum Aestivum L.)

Authors

fariba abouyee mehrizi

alireza abbassi

Ali Akbar Shah Nejat Boushehri

bahman yazdi samadi

hooshang alizade

10.22059/ijfcs.2012.29048

Abstract

Abiotic stress are the main factors causing low growth and yield in crops. Osmotic stress is an important stress and in case of salinity stress is presuming at the first step. There are different experimental and computational methods to study plant response to stress and lead to better understanding of mechanism involved in these conditions and improvement of plant tolerance to stress. Upstream analysis of genes including regulatory sequences can improve our relative knowledge about gene response to stress. To study the scope of adaptation between experimental and computational analysis and also co-regulation and co-expression ability of interested salt-response genes, we analyzed upstream regions of eight salt-response genes TSA,GCS,SOD,Tpis,AtpE,FBA,ps16 and 14-3-3p in rice and examined their expression profiling via semi-quantitative RT-PCR in bread wheat under salt stress. Our results showed two distinct groups of genes according to their expression pattern similarities. In spite of deficiency in regulatory element databases and different analyzer programs, our results showed expective adaptation between the methods.

Keywords

Bread wheat.

Co-regulation

Salinity stress

Semi-quantitative RT-PCR