Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Detection of Chromosomes Location in Barley Carrying the Genes Controlling Agronomical, Physiological and Metabolitical Indicators of Drought Tolerance using Wheat-Barley Disomic Addition Lines

Authors
ezatollah farshadfar
majid gheytooli
reza hagh parast
anita yaghooti poor
10.22059/ijfcs.2012.28494
Abstract
In order to locate the QTLs involved in the inheritance of agronomical, physiological and metabolitical indicators of drought tolerance in barley, an experiment was carried out using a wheat-barley disomic addition lines. This research was conducted in 2003-2004 at the Dry Land Agricultural Research Institute, Sararood, Kermanshah, Iran. The results of analysis of variance revealed highly significant differences for most of the investigated traits. Mean comparison exhibited that most of the genes controlling drought tolerance criteria are located on chromosomes 4H and 5H. The overall consideration of the indices using stress tolerance index (STI) and physiological multiple selection index (MSI) indicated that most of the QTLs responsible for the genetics of drought tolerance predictors are located on chromosomes 4H and 5H. A three dimensions-plot and cluster analysis confirmed the same conclusion. In correlation analysis, chlorophyll a and b and also proline were detected as indicator of drought tolerance, but excised leaf water retention (ELWR), relative water content (RWC), relative water deficit (RWD), relative water loss (RWL), chlorophyll fluorescence (CHF), cell membrane stability (CMS) and leaf chlorophyll content (LCC) are proved to be physiological indices of drought tolerance.
Keywords
Disomic addition lines
Gene location
Multiple selection index.
Stress tolerance index