A proteomic approach was conducted to further understand the mechanism of plant responses to salinity in Hordeum bulbosom. Three-week-old seedlings were treated with 300 mM of NaCl for 21 days. Total proteins of fourth leaf were extracted and separated by two-dimensional gel electrophoresis. More than 500 protein spots were reproducibly detected, including 46 spots that were up-regulated and 16 spots down-regulated. Using MALDI-TOF-TOF MS, 20 proteins involved in many cellular functions were identified. These proteins include: oxygen-evolving enhancer protein, rubisco, putative glycine decarboxylase, ribosomal protein, profilin, germin-like protein or oxalate oxidase-like protein, proxyredoxin, translationally-controlled tumor protein homology, nucleoside diphosphate kinase (NDPK), thioredoxin, dehydroascorbate reductase, 2-cys peroxiredoxin, replication factor c, and sucrose synthase. Identified proteins are involved in different metabolic pathway, such as; energy metabolism, photosynthesis, redoxin, translation, transcription, signaling, osmolite synthesis, cell wall and replication factor.
fatehi, F., hosseinzadeh, A., & alizadeh, H. (2012). The Study of Proteomic Responses of Hordeum bulbosom to Salinity Stress. Iranian Journal of Field Crop Science, 43(1), 139-150. doi: 10.22059/ijfcs.2012.24884
MLA
foad fatehi; abdolhadi hosseinzadeh; hooshang alizadeh. "The Study of Proteomic Responses of Hordeum bulbosom to Salinity Stress". Iranian Journal of Field Crop Science, 43, 1, 2012, 139-150. doi: 10.22059/ijfcs.2012.24884
HARVARD
fatehi, F., hosseinzadeh, A., alizadeh, H. (2012). 'The Study of Proteomic Responses of Hordeum bulbosom to Salinity Stress', Iranian Journal of Field Crop Science, 43(1), pp. 139-150. doi: 10.22059/ijfcs.2012.24884
VANCOUVER
fatehi, F., hosseinzadeh, A., alizadeh, H. The Study of Proteomic Responses of Hordeum bulbosom to Salinity Stress. Iranian Journal of Field Crop Science, 2012; 43(1): 139-150. doi: 10.22059/ijfcs.2012.24884
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