Effects of Gibberellic acid and Abscisic Acid on Protein Synthesis of Embryo Axis in Dormant and After-Ripened Wheat (Triticum aestivum L.RL4137) Seed



To better understand seed dormancy breaking of bread wheat (Triticum aestivum L.RL4137), we examined protein synthesis in dormant and after-ripened seeds.We used six treatments including dormant seed, dormant seed that were subjected to gibberllic acid (GA3) for 10 hours, dormant seed with one, three and six weeks of after-ripening and finally six weeks after-ripened seed subjected to abscisc acid (ABA) for ten hours. Protein synthesis was examined by two-dimensional gel electrophoresis. Almost 600 spots were displayed on gels. Volumes of 13, 40, 18, 15, 38 spots were reduced and volumes of 12, 32, 10 and 39 spots were increased significantly in treatments of GA3, and one, three and six weeks of after-ripeining, respectively. 15 spots in ABA treatment on after-ripened seed, 20 spots in dormant treatment and 23 spots in GA3 treatment were expressed. Germination was increased with after-ripening synchronously. Similar to six weeks after-ripened seed, application of GA3 also increased germination. Moreover, the effect of GA3 on up and down regulation of proteins was similar to six weeks after-ripening. ABA treatment resulted in reduction of germination in six weeks after-ripened seeds. Results of percentage of germination were similar to one week after-ripened treatment. Effect of ABA application on up and down regulation of proteins was similar to one week after-ripened seeds. The result of this study showed that GA3 had the same effect of after-ripening and ABA increased the level of seed dormancy, however, at the level of protein sysnthesis may not be similar.