Protemic Comparison of Primed and Non-Primed Wheat (Triticum aestivum L.) Seed Embryo during Germination



To better understand seed priming that improve seed germination and seedling uniformity, we examined the physiological role of osmopriming in germination of bread wheat (Triticum aestivum L.) seed embryos by a proteomic approach. For this purpose, we used two systems. The first system consisted of osmo-primed seeds by PEG 6000 at -0.8 MPa at 15?C for 24 hours, followed by air drying at 15?C, and the second corresponded to wild-type seeds. The radicle emergence was evaluated at 8-hour intervals during 88 hours in primed and non-primed seeds. Afterwards, seeds were germinated in distilled water for 32 hours at 12?C, and sampling were done in 8-hour intervals. We used the results to analyze the effects of priming on seed germination at embryo scale. The proteomic analysis indicated that priming do not participate in many processes involved in germination sensu stricto (prior to radicle protrusion). Among about 1,250 total seed proteins resolved in two-dimensional gels, changes in the abundance (up- and down-regulation) of 92 proteins were observed during germination sensu stricto. Out of this, 26 protein changes detected between primed and non-primed seed germination in embryo. In 24-hour and 32-hour intervals the least and the most changes were observed, respectively. Primary result of mass spectrometry indicated that ATP synthesis and HSP 70 increased during germination.