Identification of High Molecular Weight Proteins in Aegilops crassa from Iran

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Abstract

In this study, 120 Aegilops crassa (MMDD genome) accessions from Iran were evaluated for high molecular weight proteins. Based on SDS-page analysis, 17 bands were selected and mass spectrometry was used for protein identification. Among 17 bands selected for MALDI-TOF-TOF-MS analyzing, only 4 bands were identified as high molecular weight glutenin subunit similar to Aegilops ventricosa and Triticum aestivum with high probability. Moreove, 2 bands as beta-amylase similar to Hordeum vulgare were detected and 11 of them had no MS/MS data. The results showed that the Iranian accessions of Aegilops crassa have favorable glutenin subunits that are useful in improving bread wheat quality.

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