Molecular Basis for Resistance of Lolium rigidum Populations to Aryloxyphenoxy Propionate Herbicide through dCAPs

Dastoori, Mona; Rahimian, Hamid; Zand, Eskandar; Alizadeh, Hasan; Masoumi, Mahmoud; Bahrami, Sara

Molecular Basis for Resistance of Lolium rigidum Populations to Aryloxyphenoxy Propionate Herbicide through dCAPs

Authors

Mona Dastoori

Hamid Rahimian

Eskandar Zand

Hasan Alizadeh

Mahmoud Masoumi

Sara Bahrami

Abstract

To study the molecular basis for resistance of Lolium rigidum populations (from Fars Province) to Aryloxyphenoxy Propionate herbicide through dCAPS method, an experiment was conducted at the Biotechnology Laboratory of Shiraz University. Eighteen populations of L. rigium suspected of herbicide resistance, from Zarghan, Fasa. Noorabad and Firoozabad areas which had survived a recomended dose of Clodinafop-propargyl herbicide were tested through molecular assay. dCAPS method was carried out in three steps including DNA extraction, PCR and enzyme digestion to determine the molecular basis for herbicide resistance. The results showed that among these populations, 11 were resistant homozygous, 3 were resistant heterozygous and four ones susceptible. It is probable that substitution of isoleucine by leucine at the equilalence of residue 1781 of Acetyl CoA Carboxylase (ACCase) and mutation at position 1781 of ACCase enzyme has resulted in the high resistance in some L. rigidum populations against the mentioned herbicide.

Keywords

Acetyl CoA Carboxylase

DNA extraction

Enzymatic digestion

PCR