Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Expression profiling of genes involved in signaling process in Aegilops tauschii under salinity stress

Document Type : Research Paper

Authors
mehdi mansouri
Mohamad Reza Naghavi
Houshang Alizadeh
Ghasem Mohammadinejad
Seyed Ahmad Mousavi
Ghasem Hosseini Salekdeh
University of Tehran
10.22059/ijfcs.2016.58855
Abstract
Salinization of crop lands is one of the most important reasons for reduction in productivity and threats food security. Activation of signaling process in plants is the first step during sense environmental cues and stress signals. Identification of the components of plant intracellular signaling mechanisms, pathways, and networks in response to salt stress in Aegilops tauschii (the D genome donor of bread wheat) by transcriptome analysis tools such as RNA Seq method is very worthwhile. RNA Seq method and functional annotation of data by MapMan suite is an efficient strategy to functional genomics analyses. In order to identify and evaluate some genes involved in signaling process, we performed library construction for two cDNA libraries from leaves of seedlings treated with 200 mM NaCl and control. Results of differential expression showed that out of 4506 differentially expressed genes, 603 (11/59%) BINs assigned to signaling process. It also became clear that receptor kinases are the largest number (222 transcripts) of differentially expressed genes in signaling process components. This study is a fundamental research that provide worthwhile functional sequences for gene transferring study to improve salinity tolerant in bread wheat.
Keywords
salinity
signaling process
sequencing
RNA Seq
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Iranian Journal of Field Crop Science
Volume 47, Issue 2 - Serial Number 2
Autumn 2016
Pages 205-216

  • Receive Date 15 September 2015
  • Revise Date 10 November 2015
  • Accept Date 23 November 2015
  • Publish Date 22 August 2016