Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Study the effect of iron chelate on Chlorophyll content, Photochemical efficiency and some biochemical traits in Safflower under deficit irrigation condition

Document Type : Research Paper

Authors
Keyvan Fathi Amirkhiz 1
Majid Amini Dehaghi 2
Siavash Heshmati 1
1 Former M.Sc. Student, Departmen of Agronomy, Facultu of Agriculture, Shahed University, Tehran, Iran
2 Associate Professor, Department of Agronomy, Facultu of Agriculture,Shahed University, Tehran, Iran
10.22059/ijfcs.2015.54053
Abstract
In order to study the effect of iron chelate (Fe-EDDHA) on some biochemical characteristic of safflower (Carthamus tinctorius L.) under two irrigation regimes, a field experiment was set up a split-plot based on randomized block design with four replication in 2011 in a research field of the faculty of Agriculture at Shahed University. In this study the main plots consisted of two levels of irrigation treatments that included: full irrigation and deficit irrigation at the flowering stage (irrigation to 50% and 75% soil moisture depletion relative to field capacity, respectively). The subplots consisted of eight levels of Fe-EDDHA, half of which were soil applications (S1:0, S2:50, S3: 100 and S4:150 kg.ha-1) and the remaining half of which were foliar applications (F1:foliar application of water, F2:1000, F3:2000 and F4:3000 mg.l-1).The results showed that in desirable condition of irrigation, using of foliar application of iron chelate led to increase the chlorophyll a, chlorophyll b , total chlorophyll and chlorophyll a/b ratio. Application of low amount of iron chelate in water stress condition resulted in significant increase in chlorophyll a, total chlorophyll and carotenoids content while use of large amount of iron chelate in soil, led to increase in chlorophyll b content and Fv/Fm. In the two levels of irrigation, foliar application of iron chelate at 2000 mg.l-1 led to increase in chlorophyll a/b ratio. The results of this experiment showed that in water stress levels, with increased level of use of soil and foliar application of iron chelate, content of anthocyanins and flavonoids increased, respectively. It appears that use of iron chelate, can increase the tolerance of safflower to water deficit condition by increase in content of photosynthetic pigment.
Keywords
Chlorophyll fluorescence
Foliar Application
Iron
oxidative stress
photosynthetic pigment
Safflower
  1. Alizadeh, A. (2011). Soil, Water, Plant Relationship. Ferdowsi University of Mashhad, Iran.
  2. Allen, R. D. (1995). Dissection of oxidative stress tolerance using transgenic plants. Plant Physiology, 57, 1049-1054.
  3. Allen, R. G., Pereira, L. S., Raes, D. & Smith, M. (1998). Crop Evapotranspiration (Guidelines for Computing Crop Water Requirements). Irrigation and Drainage Paper 56. FAO, United Nations, Rome.
  4. Amal, A. M. & Aly, A. A. (2008). Alteration of some secondary metabolites and enzymes activity by using exogenous antioxidant compound in onion plants growth under seawater salt stress. Americaneurasian Journal of Science Research, 3, 139-146.
  5. Antolin, M. C., Yoller, J. & Sanchez-Diaz, M. (1995). Effects of temporary drought on nitrate-fed and nitrogen –fixing alfalfa plants. Plant Science,107,159-165.
  6. Balakumar, T., Hani Babu Vincent, V. & Paliwal, K. (1993). On the interaction of UV-B radiation (280-3 15 nm) with water stress in crop plants. Physiologia Plantarum,87, 217-222.
  7. Balouchi, H. R., Modarres Sanavy, S. A. M., Emam, Y. & Barzegar, M. (2008). Effect of Water Deficit, Ultraviolet Radiation and Carbon Dioxide Enrichment on flagLeaf Qualitative Characters of Durum Wheat (Triticum turgidum L.). Journal of Agricultural Science and Technology and Natural Resources, 12 (45), 167-181. (In Farsi)
  8. Bybordi, A. & Mamedov, G. (2010). Evaluation of Application Methods Efficiency of Zinc and Iron for Canola (Brassica napus L.). Notulae Scientia Biologicae,2 (1), 94-103.
  9. Chaves, M. M., Maroco, J. P. & Pereira, J. S. (2003). Understanding plant responses to drought from genes to the whole plant. Functional Plant Biology,30, 239-264.
  10. Chereskin, B. M. & Castelfrance, P. A. (1982). Effects of iron and oxygen on chlorophyll biosynthesis II. Observation on the biosynthetic pathway in isolatedetio-chloroplasts. Plant Physiology,68, 112-116.
  11. Curie, C. & Briat J. F. (2003). Iron transport and signaling in plants. Annual Review of Plant Biology,54, 183-206.
  12. Ghasemi-Fasaei, R., Ronaghi, A. Maftoun, M., Karimianand, N. A. & Soltanpour, P. N. (2005). Iron Manganese Interaction in Chickpea as Affected by Foliar and Soil Application of Iron in a Calcareous Soil. Communications in Soil Science and Plant Analysis, 36, 1717-1725. (In Farsi)
  13. Gogor Cena, Y., Abadía, J. & Abadía, A. (2004). A new technique for screening iron-efficient genotypes in peach rootstocks elicitation of root ferric chelate reductase by manipulation of external iron concentrations.  Journal of Plant Nutrition,27, 1701-1715.
  14. Grace, S. C. & Logan, B. A. (2000). Energy dissipation and radical scavenging by the plant phenylpropanoid pathway. Philosophical Transactions of the Royal Society B,355, 1499-1510.
  15. Hansch, R. & Mendel, R. R. (2009). Physiological functions of mineral micronutrients (Cu, Zn, Mn, Fe, Ni, Mo, B, Cl). Current Opinion in Plant Biology, 12, 259-266.
  16. Henriques, F. S. (2003).Gas exchange, chlorophyll a fluorescence kinetics and lipid peroxidation of pecan leaves with varying manganese concentrations. Plant Science, 165, 239-244.
  17. Jagtap, V., Bhargava, S., Sterb, P. & Feierabend, J. (1998). Comparative effect of water, heat and light stresses on photosynthetic reactions in Sorghum tricolor (L.) Moench. Journal of Experimental Botany,49, 1715-1721.
  18. Jaleel, C. A., Gopi, R. Sankar, B., Gomathinayagam,M. & Panneerselvam, R. (2008). Differential responses in water use efficiency in two varieties of Catharanthus roseus under drought stress. Comptes Rendus Biologies, 33, 42-47.
  19. Krause, G. H. & Weis, E. (1991). Chlorophyll fluorescence and photosynthesis: The basics. Annual Review of Plant Physiology and Plant Molecular Biology, 42, 313-349.
  20. Krizek, D. T., Kramer, G. F., Upadhyaya A. & Mirecki, R. M. (1993). UV-B Response of cucumber seedling grown under metal halid and high pressure sodium/deluxe lamps. Plant Physiology, 88, 350-358.
  21. Kruk, J., Czytko, H. H., Oettmeier, W. & Trebest, A. (2005). Tocopherol as singlet oxygen scavenger in photosystem II. Journal of Plant Physiology, 162, 749-757.
  22. Leng, P., Itamura, H., Yamamura, H. & M. Deng, X. (2000). Anthocyanin accumulation in apple and peach shoots during cold acclimation. Scientia Horticulturae, 83, 43-50.
  23. Lichtenthaler, H. K. (1987). Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes. Methods in Enzymology, 148, 350-382.
  24. Marschner, H. (1995). Mineral Nutrition of Higher Plants (2th Ed.). Academic Press, London.
  25. Miladi Lari, A. & Ehsanzadeh, P. (2010). The Negative Effect of Drought on Safflower Grain Yield through Impact on Photosynthetic Surfaces and on Efficiency. Iranian Journal of Field Crop Science, 41 (2), 375-384. (In Farsi).
  26. Mohsenzadeh, S., Malboobi, M. A., Razavi, K. & Farrahi-Aschtiani, S. (2006). Physiological and molecular responses of Aeluropus lagopoides (Poaceae) to water deficit. Environmental and Experimental Botany, 56,314-322.
  27. Movahhedy Dehnavy, M., Modarres Sanavi, S. A. M., Sorush Zadehand, A. & Jalali, M. (2004). Changes in proline, total soluble sugars, SPAD and chlorophyll fluorescence in winter safflower cultivars under drought stress and foliar application of zink and manganese. Journal of Dessert, 9(1), 93-109. (In Farsi)
  28. Nasibi, F. & M-Kalantari, K. H. (2005). The effects of UV-a, UV-b and UV-c on protein and ascorbate content, lipid peroxidation and biosynthesis of screening compounds in Brassica Napus. Iranian Journal of Science and Technology Transaction A- Science, 29(1), 39-48. (In Farsi)
  29. Navari-Izoo, F., Quartacci, M. F. & Izzo, R. (1990). Water-stress induced changed changes in protein and free amino acids in field grown maize and sun flower. Plant Physiology and Biochemistry, 28, 531-537.
  30. Pattangual, W. & Madore, M. (1999). Water deficit effects on raffinose family oligosaccharide metabolism in Coleus. Plant Physiology, 121, 998-993.
  31. Pessarakli, M. (1999). Hand book of Plant and Crop Stress. Marcel Dekker Inc. pp.697.
  32. Quiles, M. J. & López, N. L. (2004). Photoinhibition of photosystems I and II induced by exposure to high light intensity during oat plant grown effects on the chloroplastic NADH dehydrogenase complex. Plant Science, 166, 815-823.
  33. Romheld, V. & Marschner, H. (1991). Function of micronutrients in plants.In.J. J. Mortvedt, F. R. Cox, L. M. Shuman, & R. M. Welch, Micronutrients in Agriculture, (2th ed.), (pp.297-328).Madison,WI:SSSA.
  34. Sgherri, C. L. M. & Navari-Izzo F. (1995). Sunflower seedlings subjected to increasing water deficit stress: oxidative stress and defence mechanisms. Physiologia Plantarum, 93, 25-30.
  35. Sharma, P. K. & Hall, D. O. (1991). Interaction of salt stress and photoinhibition on photosynthesis in barley and sorghum. Journal of Plant Physiology, 138(5), 614-619.
  36. Sofo, A., Dichio, B., Xiloyannis C. & Masia A. (2004). Effects of different irradiance levels on some antioxidant enzymes and on malondialdehyde content during rewatering in olive tree. Plant Science,166, 293-30.
  37. Yang, J., Zhang, J., Wang, Z., Zhu, Q. & Liu, L. (2001). Water deficit induced senescence and its relationship to the remobilization of pre-stored carbon in wheat during grain filling. Agronomy Journal, 93, 196-206.
  38. Yordanov, I., Velikova, V. & Tsonev, T. (2003). Plant responses to drought and stress tolerance. Bulgarian Journal of Plant Physiology, 187-206.
Iranian Journal of Field Crop Science
Volume 46, Issue 1 - Serial Number 1
Spring 2015
Pages 137-145

  • Receive Date 15 December 2014
  • Revise Date 17 February 2015
  • Accept Date 04 March 2015
  • Publish Date 21 March 2015