Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Investigation of Genetic Diversity in Chickpea (Cicer arietinum L.) Lines Using ISSR Markers

Document Type : Research Paper

Authors
Heidar Naderi 1
Majid Shokrpour 2
Ali Asghari 3
Homayoun Kanooni 4
Ezatollah Esfandiari 5
1 Former Graduate Student, Faculty of Agriculture, University of Mohaghegh Ardabili, Aradabil
2 Assisstant Professor, University College of Agriculture and Natural Resources, University of Tehran, Karaj
3 Associate Professor, Faculty of Agriculture, University of Mohaghegh Ardabili, Aradabil
4 Assisstant Professor, Research Center of Agriculture, Kordestan
5 Associate Professor, Faculty of Agriculture, University of Maragheh, Maragheh
10.22059/ijfcs.2014.53561
Abstract
In this research, ISSR technique was applied to assess genetic diversity among 56 chickpea lines. Out of 34 primers, nine primers produced appropriate banding patterns and produced 35 polymorphic bands in chickpea lines. Mean percentage of polymorphism of the primers was 66.01 with range of 25 to 100 percent. Mean of Nei's genetic diversity and Shannon information index over all primers were 0.325 and 0.492, respectively. Based on Jaccard's similarity coefficient, lines of FLIP01-40C and FLIP02-84 had the lowest similarity (0.166) and lines of FLIP03-6C and FLIP03-8C had the highest similarity coefficient Cluster analysis using UPGMA method and Jaccard's similarity matrix was done. The dendrogram was cut at 0.51 scaled interval and categorized the lines in six distinct groups. Distinct grouping and large genetic distances among the chickpea lines stated a good molecular variation. Showing the lines by a tri-plot based on the three first coordinates from PCoA, verified the clustering and efficiently separated them
Keywords
Chickpea
Cluster Analysis
genetic diversity and ISSR markers
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Iranian Journal of Field Crop Science
Volume 45, Issue 4 - Serial Number 4
Spring 2015
Pages 509-519

  • Receive Date 21 October 2013
  • Revise Date 19 August 2014
  • Accept Date 17 September 2014
  • Publish Date 22 December 2014