Iranian Journal of Field Crop Science

Iranian Journal of Field Crop Science

Identification of low molecular weight glutenin subunits in Triticum aestivum and the D genome of wild related wheat

Document Type : Research Paper

Authors
Seyvan Ahmadi 1
Mohammad-Reza Naghavi 2
Ali Akbar Shah Nejat Booshehri 2
1 Graduated Student, University College of Agriculture & Natural Resources, University of Tehran
2 Professor, University College of Agriculture & Natural Resources, University of Tehran
10.22059/ijfcs.2014.53529
Abstract
In this study, 110 samples of bread wheat, Aegilops crassa, Ae. cylindrica and Ae. tauschii were investigated to identify Low-Molecular-Weight Glutenin Subunits. Overall, the four alleles in term of the analysis of SDS-PAGE gels were detected. The ʻaʼ allele with 45% has the greatest frequency, and after that, ʻbʼ, ʻcʼ and ʻdʼ alleles were with 38.1%, 11.8% and 9.1% frequencies, respectively; however, in Ae. crassa, ʻbʼ and ʻcʼ alleles are the highest and lowest frequencies, respectively. The ʻaʼ and (ʻcʼ and ʻdʼ) alleles are the highest and lowest frequencies in Ae. cylindrica; nonetheless, In Ae. tauschii, ʻaʼ and ʻdʼ are the highest and lowest frequencies. In T. aestivum, ʻbʼ are the highest while ʻdʼ shows the lowest frequencies. Genetic diversity (ζ) in populations of bread wheat, Ae. crassa, Ae. cylindrica, Ae. tauschii and total population were calculated 0.6564, 0.5792, 0.6378, 0.6214 and 0.6294, respectively. Furthermore, the average genetic diversity (H) for the total population was obtained 0.6294. The results show that wild wheat relatives have various alleles that can be utilized them in breeding programs to improve glutenin.
Keywords
Low-Molecular-Weight Glutenin Subunit
D-genome
SDS-PAGE
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Iranian Journal of Field Crop Science
Volume 45, Issue 3 - Serial Number 3
Autumn 2014
Pages 327-334

  • Receive Date 06 January 2013
  • Revise Date 19 May 2015
  • Accept Date 07 August 2013
  • Publish Date 23 September 2014