High molecular weight glutenin subunit composition in Triticum boeoticum and Aegilops taushii in Iran and neighbor countries

Keyhani, Tina; Shahnejat-Bushehri, Ali-Akbar; Naghavi, Mohammad Reza

doi:10.22059/ijfcs.2014.51908

High molecular weight glutenin subunit composition in Triticum boeoticum and Aegilops taushii in Iran and neighbor countries

Document Type : Research Paper

Authors

Tina Keyhani ¹

Ali-Akbar Shahnejat-Bushehri ²

Mohammad Reza Naghavi ²

¹ Msc., Agricultural Biotechnology, University of Tehran, Iran

² Professor, Department of Agronomy and Plant Breeding, Collage of Agricultural and Natural Resources, University of Tehran, Iran

10.22059/ijfcs.2014.51908

Abstract

The high molecular weight glutenin subunit (HMW-GS) compositions of 19 diploid wild wheat (T.boeoticum) and 36 diploid wild wheat (A.tauschii) were determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). All of T. boeoticum were characterized for their HMW-GS, showed 2^* in their Glu-A1 locus. In the study of Aegilops tauschii that were collected from Iran and 7 neighboring countries, high allelic diversity was absorved. Six alleles were identified at Glu-D1 loci in Iranian Aegilops tauschii and four alleles were founded for Glu-D1 locus in Aegilops tauschii in neighboring countries. In addition, some rare subunits combinations such as 2+10, 2+11 and 11 were found in this study, that were new subunits in compared with previous studies I Irani. Also, subunits 1.5+12 and 2+T₂ were the scarcity subunits that were detected in Aegilops tauschii of neighboring countries.

Keywords

bread making quality

Genetic diversity

HMW-Glutenin

SDS-PAGE