Expression profiling of genes involved in signaling process in Aegilops tauschii under salinity stress

Document Type : Research Paper

Authors

University of Tehran

Abstract

Salinization of crop lands is one of the most important reasons for reduction in productivity and threats food security. Activation of signaling process in plants is the first step during sense environmental cues and stress signals. Identification of the components of plant intracellular signaling mechanisms, pathways, and networks in response to salt stress in Aegilops tauschii (the D genome donor of bread wheat) by transcriptome analysis tools such as RNA Seq method is very worthwhile. RNA Seq method and functional annotation of data by MapMan suite is an efficient strategy to functional genomics analyses. In order to identify and evaluate some genes involved in signaling process, we performed library construction for two cDNA libraries from leaves of seedlings treated with 200 mM NaCl and control. Results of differential expression showed that out of 4506 differentially expressed genes, 603 (11/59%) BINs assigned to signaling process. It also became clear that receptor kinases are the largest number (222 transcripts) of differentially expressed genes in signaling process components. This study is a fundamental research that provide worthwhile functional sequences for gene transferring study to improve salinity tolerant in bread wheat.

Keywords

References

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Iranian Journal of Field Crop Science
Volume 47, Issue 2 - Serial Number 2
October 2016
Pages 205-216

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History

  • Receive Date: 15 September 2015
  • Revise Date: 10 November 2015
  • Accept Date: 23 November 2015
  • Publish Date: 22 August 2016

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