To evaluate allelic diversity at loci encoding low molecular weight glutenin subunits (LMW-GS) among 62 genotypes of bread wheat, nine specific primer pairs for Glu-A3, Glu-B3, Glu-D3 loci were employed. PCR products were separated on 2% agarose gels. Two pairs of specific primers, at amplified Glu-A3 locus, 8 alleles were detected the c allele of which was the most abundant with a frequency of 0.311.Seventeen alleles were detected at Glu-B3 loci by through specific primers, the f allele of which was the most abundant with a frequency of 0.145. For Glu-D3 loci; in total 22 alleles were detected by four specific primers the h allele of which was the most abundant with a frequency of 0.174. Using Nei’s genetic variation index (H), the genetic diversity for Glu-A3, Glu-B3 and Glu-D3 loci, were estimated as 0.818, 0.914 and 0.915 respectively. The mean genetic diversity (H) for Glu-3 loci was 0.894 among the genotypes. Genetic variation index (H) for each primer revealed that Glu-D3.3 primer exhibits more polymorphism than the other primers. Related analysis of quality and molecular data through use of stepwise regression method indicated that the highest number of informative markers belong to dry and wet gluten. The results revealed that there is a considerable diversity among Iranian bread wheat genotypes which could be employed as a valuable source for improving the quality of wheat final products.