The effect of cold plasma treatment on seed germination and morphological characteristics of seedlings of two ecotypes of Black Cumin (Nigella sativa L.)

Document Type : Research Paper

Authors

1 Department of Agronomy and Plant Breeding Sciences, College of Aburaihan, University of Tehran, Pakdasht, Tehran, Iran

2 Laser and Plasma Research Institute, Shahid Beheshti University, Tehran, Iran.

Abstract

In order to investigate the effect of cold plasma on the seed germination and emergence of Black Cumin, an experiment was conducted in 2022 in the form of a completely randomized design with four replications on two ecotypes of Black Cumin, Dargaz and Yazd. The treatments include the control treatment (without cold plasma), cold plasma treatment based on gliding arc in 30s, 1, 2 and 3 minutes on Dargaz ecotype; and control treatment, radio frequency (RF) plasma treatments with power of: 50W in 5 minutes, 50W in 15 minutes and 100W in 5 minutes and cold plasma treatment based on Surface dielectric barrier discharge (SDBD) in 10, 15 and 20 seconds on Yazd ecotype. The results showed that in Dargaz ecotype, gliding arc treatment in 3 minutes with 67.5% and in Yazd ecotype, RF treatment with a power of 50W in 5 minutes with 68.5% achieved the highest germination percentage. In Dargaz ecotype, gliding arc treatment in 30s and in Yazd ecotype, SDBD treatment in 20s caused the maximum seedling length. In general, the results of this research showed that cold plasma improved the seed germination and morphological characteristics of Black Cumin seedlings in both ecotypes.

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