Univrsity of Tehran Press Iranian Journal of Field Crop Science 2008-4811 53 4 2022 12 22 Evaluation of some physiological and biochemical traits of wild species and wheat possessing the D genome under water deficit stress. Evaluation of some physiological and biochemical traits of wild species and wheat possessing the D genome under water deficit stress. 77 91 91635 10.22059/ijfcs.2021.328612.654848 FA Hamid Bayat Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Iran Mansoor Omidi Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Iran Ali Akbar Shah Nejat Boushahri Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Iran 0000-0003-2558-9598 Mohammad Reza Naghavi Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Iran Journal Article 2021 08 31 Water deficit as one of the main environmental factors reduces plants yield around the world. Wheat is one of the most important cereal providing human’s food demand. Aegilops is a wild species belonging to the wheat family that can be used as a suitable germplasm to discover new genes to improve wheat tolerance to biotic and abiotic stresses in breeding programs. In this regard, 3 different species of Ae. Tauschii, Ae. Cylindrical, and Ae. Crassa , as well as wheat were cultivated under normal and water- deficit stress (FC = 25%). Morphological, physiological, biochemical, and growth indices were used to assess stress tolerance. Given the results, leaf length, width and area in plants under stress decreased sharply which the sensitive cultivar, Darya, showed the minimum value. Based on. biochemical analysis, under stress, peroxidase and catalase enzymes activities were increased either in wheat or in Aegilops, while ascorbate peroxidase decreased only in wheat in contrast to aegilops. A negative correlation was observed between catalase and ascorbate peroxidase under water-deficit stress. Among growth indices, wet and dry biomass rarely decreased in Cylindric species. According to multifactorial analysis, Ae. Cylindrical and Ae. Crassa species were more tolerant than wheat. Utilizing these could be useful to develop the tolerant cultivars to abiotic stress such as water- deficit. Water deficit as one of the main environmental factors reduces plants yield around the world. Wheat is one of the most important cereal providing human’s food demand. Aegilops is a wild species belonging to the wheat family that can be used as a suitable germplasm to discover new genes to improve wheat tolerance to biotic and abiotic stresses in breeding programs. In this regard, 3 different species of Ae. Tauschii, Ae. Cylindrical, and Ae. Crassa , as well as wheat were cultivated under normal and water- deficit stress (FC = 25%). Morphological, physiological, biochemical, and growth indices were used to assess stress tolerance. Given the results, leaf length, width and area in plants under stress decreased sharply which the sensitive cultivar, Darya, showed the minimum value. Based on. biochemical analysis, under stress, peroxidase and catalase enzymes activities were increased either in wheat or in Aegilops, while ascorbate peroxidase decreased only in wheat in contrast to aegilops. A negative correlation was observed between catalase and ascorbate peroxidase under water-deficit stress. Among growth indices, wet and dry biomass rarely decreased in Cylindric species. According to multifactorial analysis, Ae. Cylindrical and Ae. Crassa species were more tolerant than wheat. Utilizing these could be useful to develop the tolerant cultivars to abiotic stress such as water- deficit. https://ijfcs.ut.ac.ir/article_91635_7b1fafd9ff99d4d21b700703ab7a4318.pdf