Univrsity of Tehran Press Iranian Journal of Field Crop Science 2008-4811 53 1 2022 03 21 The chlorophyll fluorescence parameters response of lentil (Lens culinaris Medik.) genotypes to freezing stress The chlorophyll fluorescence parameters response of lentil (Lens culinaris Medik.) genotypes to freezing stress 69 83 84743 10.22059/ijfcs.2021.313523.654771 FA Jafar Nabati Research Center for Plant Sciences, Ferdowsi University of Mashhad, Iran 0000-0003-0483-7003 Ahmad Nezami Faculty of Agriculture and Research Center for Plant Sciences, Ferdowsi University of Mashhad, Iran. 0000-0001-9490-6935 Syadeh Mahbobe Mirmiran Khorasan-e-razavi Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran Alireza Hasanfard Faculty of Agriculture, Ferdowsi University of Mashhad, Iran 0000-0002-5256-6394 Mohammad Javad Ahmadi Lahijani Faculty of Agriculture, Ferdowsi University of Mashhad, Iran Journal Article 2020 11 15 A factorial experiment in a randomized complete block design with three replications was carried out to evaluate the freezing tolerance of 40 lentil genotypes, at the Research Center for Plant Science, Ferdowsi University of Mashhad, in 2017-2018. The status of chlorophyll fluorescence parameters was studied in eight genotypes (seven tolerant: MLC11, MLC12, MLC17, MLC38, MLC95, MLC409, and MLC410 and one intolerant: MLC9) and five time periods (before stress, 12, 24, 48, and 72 hours) exposed to freezing temperatures (-13, -15 and -18°C) for one hour. The results showed that the value of F'm in MLC409, 72 hours after freezing stress, was slightly different from its value in the early hours. The maximum efficiency of photosystem II (F′v/F′m) was not affected by temperature decline from -13 to -18°C. MLC9 F′v/F′m, however, decreased by 26% when the temperature declined from -13 to -18°C. Furthermore, F′v/F′m decreased by 14% at -18°C 24h after the freezing stress, which was the greatest decrease compared to -13 and -15°C. However, 48 h after freezing stress, the optimal recovery trend was evident at all temperatures. Although the operational efficiency of photosystem II (F′q/F′m) decreased at all three temperatures, 24 h after the freezing stress, this decrease was greater at -18°C than at other temperatures. Except for the MLC9, other genotypes, especially MLC12, could improve freezing stress damage 24 h after stress and gradually return to F′v/F′m before freezing stress. In general, the freezing-tolerant genotypes and in addition to the survival rate of more than 80% at -18°C, showed a high potential to restore the photosystemII performance, which can be considered for further studies for autumn cultivation in cold and highland regions.. A factorial experiment in a randomized complete block design with three replications was carried out to evaluate the freezing tolerance of 40 lentil genotypes, at the Research Center for Plant Science, Ferdowsi University of Mashhad, in 2017-2018. The status of chlorophyll fluorescence parameters was studied in eight genotypes (seven tolerant: MLC11, MLC12, MLC17, MLC38, MLC95, MLC409, and MLC410 and one intolerant: MLC9) and five time periods (before stress, 12, 24, 48, and 72 hours) exposed to freezing temperatures (-13, -15 and -18°C) for one hour. The results showed that the value of F'm in MLC409, 72 hours after freezing stress, was slightly different from its value in the early hours. The maximum efficiency of photosystem II (F′v/F′m) was not affected by temperature decline from -13 to -18°C. MLC9 F′v/F′m, however, decreased by 26% when the temperature declined from -13 to -18°C. Furthermore, F′v/F′m decreased by 14% at -18°C 24h after the freezing stress, which was the greatest decrease compared to -13 and -15°C. However, 48 h after freezing stress, the optimal recovery trend was evident at all temperatures. Although the operational efficiency of photosystem II (F′q/F′m) decreased at all three temperatures, 24 h after the freezing stress, this decrease was greater at -18°C than at other temperatures. Except for the MLC9, other genotypes, especially MLC12, could improve freezing stress damage 24 h after stress and gradually return to F′v/F′m before freezing stress. In general, the freezing-tolerant genotypes and in addition to the survival rate of more than 80% at -18°C, showed a high potential to restore the photosystemII performance, which can be considered for further studies for autumn cultivation in cold and highland regions..

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