Univrsity of Tehran Press Iranian Journal of Field Crop Science 2008-4811 52 1 2021 03 21 Effect of foliar application of zinc, iron, and manganese on morphological, and phytochemical traits of hyssop (Hyssopus officinalis L.) Effect of foliar application of zinc, iron, and manganese on morphological, and phytochemical traits of hyssop (Hyssopus officinalis L.) 1 11 80802 10.22059/ijfcs.2017.132493.653947 FA Maryam Chalrkaei Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Alireza Abbasi Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, 0000-0003-3598-0419 Sedigheh Yusefi Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Davood Dadashi Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources, University of Tehran, Journal Article 2015 10 04 Acidification of cell wall is the most valid cause of fast cell expansion. Expansins are a kind of wall loosening protein that can expand wall through pH changes. In this study AtEXPB2 genewas transferred to tobacco plant. Regenerated plants were cultured in selective medium (contained kanamycin) then transgenic plants were selected and afterward transmitted to greenhouse. To confirm transgenic plant DNA was extracted from them and PCR was down via specific primer of AtEXPB2. Seed was collected from transgenic plants and seed of three transgenic lines were cultured in MS selective medium. Transgenic seedlings tolerate medium which contain kanamycin. Subsequently some of these seedlings were grown in greenhouse and others were grown in Hoagland nutrient solution for evaluating root morphology. The results indicated that transgenic lines had bigger organs in most of the measured traits. For example capsule number, total seed weight, root length, root fresh weight and so on were increased in transgenic lines in comparison to wild type plant. These results suggested a specific role for this gene as a common growth regulator that could be used to produce transgenic plants with larger organs as well as tolerant to stresses like drought and salinity. Acidification of cell wall is the most valid cause of fast cell expansion. Expansins are a kind of wall loosening protein that can expand wall through pH changes. In this study AtEXPB2 genewas transferred to tobacco plant. Regenerated plants were cultured in selective medium (contained kanamycin) then transgenic plants were selected and afterward transmitted to greenhouse. To confirm transgenic plant DNA was extracted from them and PCR was down via specific primer of AtEXPB2. Seed was collected from transgenic plants and seed of three transgenic lines were cultured in MS selective medium. Transgenic seedlings tolerate medium which contain kanamycin. Subsequently some of these seedlings were grown in greenhouse and others were grown in Hoagland nutrient solution for evaluating root morphology. The results indicated that transgenic lines had bigger organs in most of the measured traits. For example capsule number, total seed weight, root length, root fresh weight and so on were increased in transgenic lines in comparison to wild type plant. These results suggested a specific role for this gene as a common growth regulator that could be used to produce transgenic plants with larger organs as well as tolerant to stresses like drought and salinity.

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