Univrsity of Tehran Press Iranian Journal of Field Crop Science 2008-4811 47 1 2016 05 21 Expression levels of hdr, fds and idi genes in aerial organs of Artemisia diffusa and their relationships with some terpene contents Expression levels of hdr, fds and idi genes in aerial organs of Artemisia diffusa and their relationships with some terpene contents 19 27 63585 10.22059/ijfcs.2016.63585 FA Farzad Alaeimoghadam Former M.Sc. Student of Agriculture Biotechnology, Department of Agronomy, University College of Agriculture & Natural Resources of University of Tehran, Karaj, Iran Mohamad Reza Naghavi Professor of Agriculture Biotechnology, Department of Agronomy, University College of Agriculture & Natural Resources of University of Tehran, Karaj, Iran Abdol Hadi Hossein Zadeh Associate Professor, Department of Agronomy, University College of Agriculture & Natural Resources of University of Tehran, Karaj, Iran Journal Article 2014 11 25 In view of the importance of terpene in human’s life, analyzing the relationship between them and the involved genes are of crucial necessity. The necessity is for exploring influential genes. Among different plants containing terpene, Artemisia is included. In this study, several genes Farnesyl diphosphate synthase (fds), Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (hdr) and Isopentenyl diphosphate isomerase (idi) in pathways 2-c-methyl-erythritol 4- phosphate (MEP) and Mevalonate (MVA) were analyzed in comparison to some monoterpene and sesquiterpene content in three tissues namely leaf, bud and flower of Artemisia diffusa. This study was conducted to compare RT-PCR data of genes with data from terpene GC-MS results. Results showed that it seemed for certain terpenes production in certain tissues, the above genes had limiting function (as idi in bud for 1,8- cineole and germacrene-D). These genes play important roles in terpene production and for some other terpenes, probably, other downstream genes such as terpene synthase had this limiting role (about beta-farnescene, for example, genes idi and fds had no limiting role in none of tissues mentioned above). Another point which casts doubt on fds, hdr and idi functions in terpene production is post-transcriptional regulation of these genes, the factor which was not evaluated in this study. In view of the importance of terpene in human’s life, analyzing the relationship between them and the involved genes are of crucial necessity. The necessity is for exploring influential genes. Among different plants containing terpene, Artemisia is included. In this study, several genes Farnesyl diphosphate synthase (fds), Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (hdr) and Isopentenyl diphosphate isomerase (idi) in pathways 2-c-methyl-erythritol 4- phosphate (MEP) and Mevalonate (MVA) were analyzed in comparison to some monoterpene and sesquiterpene content in three tissues namely leaf, bud and flower of Artemisia diffusa. This study was conducted to compare RT-PCR data of genes with data from terpene GC-MS results. Results showed that it seemed for certain terpenes production in certain tissues, the above genes had limiting function (as idi in bud for 1,8- cineole and germacrene-D). These genes play important roles in terpene production and for some other terpenes, probably, other downstream genes such as terpene synthase had this limiting role (about beta-farnescene, for example, genes idi and fds had no limiting role in none of tissues mentioned above). Another point which casts doubt on fds, hdr and idi functions in terpene production is post-transcriptional regulation of these genes, the factor which was not evaluated in this study.

https://ijfcs.ut.ac.ir/article_63585_bb61892f0f6458e2dd57aa37e115e56f.pdf