Univrsity of Tehran Press Iranian Journal of Field Crop Science 2008-4811 46 2 2015 06 22 Proteome analysis of wheat seed embryo (Tritium aestivum) in tolerant and susceptible cultivars under drought stress during early germination phase Proteome analysis of wheat seed embryo (Tritium aestivum) in tolerant and susceptible cultivars under drought stress during early germination phase 207 215 54868 10.22059/ijfcs.2015.54868 FA Ali Shayanfar Former Graduate Student, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj Reaza Tavakkol Afshari Professor, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj Houshang Alizade Assistant Professor, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj 0000-0001-8138-0786 Abolrahman Rasoulnia Former Graduate Student, Seed Science and Technology, College of Agriculture & Natural Resources, University of Tehran, Karaj Journal Article 2014 09 14 Germination is one the most important growth phases in plants that injury from drought stress extremely. Germination divided to 3 phases although before third phases, many activity (radicle protrusion) initiated. Many metabolically activity occurred specially in first phase since seed embryo is a live organ, although differences in protein patterns arised in this phase. This study was carried out to understand the effect of drought stress on protein patterns in the first phase of germination. In order to analysis the effects of drought stress (-12 bar) on wheat embryo proteome (Sardari and Qods respectively tolerant and susceptiblecultivar), factorial experiment was conducted in a Randomized completely block design with three replications. Drought stress diminished germination percentage and caused adverse effects on the proteins pattern. Two-dimensional gel electrophoresis analysis of wheat embryo axis showed 1000 spots were reproducible that 25 spots between treatments indicated significant differences on 5درصد level. In control condition, in comparison tolerant to susceptiblecultivar, 22 and 6 spots and in stress condition, 21 and 10 spots were down and up regulated respectively. The results indicated that frequency of protein under drought stress depends on severity and amount of stress and imbibition time.   Germination is one the most important growth phases in plants that injury from drought stress extremely. Germination divided to 3 phases although before third phases, many activity (radicle protrusion) initiated. Many metabolically activity occurred specially in first phase since seed embryo is a live organ, although differences in protein patterns arised in this phase. This study was carried out to understand the effect of drought stress on protein patterns in the first phase of germination. In order to analysis the effects of drought stress (-12 bar) on wheat embryo proteome (Sardari and Qods respectively tolerant and susceptiblecultivar), factorial experiment was conducted in a Randomized completely block design with three replications. Drought stress diminished germination percentage and caused adverse effects on the proteins pattern. Two-dimensional gel electrophoresis analysis of wheat embryo axis showed 1000 spots were reproducible that 25 spots between treatments indicated significant differences on 5درصد level. In control condition, in comparison tolerant to susceptiblecultivar, 22 and 6 spots and in stress condition, 21 and 10 spots were down and up regulated respectively. The results indicated that frequency of protein under drought stress depends on severity and amount of stress and imbibition time.   https://ijfcs.ut.ac.ir/article_54868_03d740d48e733bd8058f332a5f7254c2.pdf