Univrsity of Tehran Press Iranian Journal of Field Crop Science 2008-4811 44 4 2013 12 22 Cloning of WDREB2 Transcription Factor in pBI121 Expression Vector and Transfer to Tomato In Order To Improve Cold Stress Resistance Cloning of WDREB2 Transcription Factor in pBI121 Expression Vector and Transfer to Tomato In Order To Improve Cold Stress Resistance 635 644 50333 10.22059/ijfcs.2013.50333 FA Sima Sazgari Graduate student, College of Agriculture, University of Shiraz, Shiraz, Iran Ali Niyazi Assistant Professor, College of Agriculture, University of Shiraz, Shiraz, Iran Journal Article 2012 06 20 Abiotic stress such as cold and drought has adverse effects on plants growth and development. Abiotic stress resistance is one of the most important goals in plant breeding. This goal can be achieved through transcription factor gene transfer to plants. Recent studies show many successes in improving abiotic stress resistance by use of transcription factor gene transfer. The objective of the research was to clone wheat alpha isoform of <em>WDREB2</em>, effective abiotic stress responsive element, in suitable expression vector, and its transfer to tomato in order to improve cold stress resistance. The gene was cloned in expression vector pBI121, and transferred to tomato through <em>Agrobacterium tumefaciens </em>C58 strain. In order to admit transformation, DNA extraction and PCR with specific primer was done on transgenic and nontransgenic tomato plants. Verification of <em>WDREB2</em> expression was done by RT- PCR after RNA extraction and cDNA synthesis. Bioassay test of transgenic plants under cold stress treatment was performed. Phonotypical comparison between transgenic and normal plants under cold stress confirmed cold stress resistance of transgenic lines. Abiotic stress such as cold and drought has adverse effects on plants growth and development. Abiotic stress resistance is one of the most important goals in plant breeding. This goal can be achieved through transcription factor gene transfer to plants. Recent studies show many successes in improving abiotic stress resistance by use of transcription factor gene transfer. The objective of the research was to clone wheat alpha isoform of <em>WDREB2</em>, effective abiotic stress responsive element, in suitable expression vector, and its transfer to tomato in order to improve cold stress resistance. The gene was cloned in expression vector pBI121, and transferred to tomato through <em>Agrobacterium tumefaciens </em>C58 strain. In order to admit transformation, DNA extraction and PCR with specific primer was done on transgenic and nontransgenic tomato plants. Verification of <em>WDREB2</em> expression was done by RT- PCR after RNA extraction and cDNA synthesis. Bioassay test of transgenic plants under cold stress treatment was performed. Phonotypical comparison between transgenic and normal plants under cold stress confirmed cold stress resistance of transgenic lines. https://ijfcs.ut.ac.ir/article_50333_eeceab35f5e0b21c53a7c5868d1b08cb.pdf